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Literature summary for 2.4.2.28 extracted from

  • Cacciapuoti, G.; Porcelli, M.; Bertoldo, C.; De Rosa, M.; Zappia, V.
    Purification and characterization of extremely thermophilic and thermostable 5'-methylthioadenosine phosphorylase from the archaeon Sulfolobus solfataricus. Purine nucleoside phosphorylase activity and evidence for intersubunit disulfide bonds (1994), J. Biol. Chem., 269, 24762-24769.
    View publication on PubMed
Search for more literature for 2.4.2.28

General Stability

General Stability Organism
no loss of activity after 24 h at room temperature in presence of 9 M urea, 4 M guanidine hydrochloride, 0.075% SDS, 50% methanol, 50% ethanol, 50% dimethylformamide, 1 M NaCl, and 1% Triton X-100 Saccharolobus solfataricus
no loss of activity after treatment with thermolysin, trypsin, and chymotrypsin for 24 h at 37°C Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
iodoacetate incorporation of 12 mol iodoactate per mol of enzyme Saccharolobus solfataricus
additional information activity is not affected by alkylating, mercaptide-forming and oxidizing thiol reagents; no inhibition by S-adenosyl-L-methionine Saccharolobus solfataricus
S-adenosyl-L-homocysteine no inhibition Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 5'-methylthioadenosine
-
 Saccharolobus solfataricus
0.123
-
 phosphate
-
 Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Saccharolobus solfataricus 5829
-
cytosol exclusively Saccharolobus solfataricus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
 6 * 27000, SDS-PAGE Saccharolobus solfataricus
160000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5'-methylthioadenosine + phosphate Saccharolobus solfataricus
-
 adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
adenosine + phosphate Saccharolobus solfataricus
-
 adenine + D-ribose 1-phosphate
-
 ?
guanosine + phosphate Saccharolobus solfataricus
-
 guanine + D-ribose 1-phosphate
-
 ?
inosine + phosphate Saccharolobus solfataricus
-
 hypoxanthine + D-ribose 1-phosphate
-
 ?
additional information Saccharolobus solfataricus involved in the catabolism of 5'-methylthioadenosine, adenosine, guanosine and inosine ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
1-propanol no loss of activity after 24 h at room temperature in presence of 50% methanol, loss of 90% activity at 90°C after 1 h Saccharolobus solfataricus
acetonitrile loss of 89% activity at 70°C after 1 h Saccharolobus solfataricus
dimethylformamide no loss of activity after 24 h at room temperature in presence of 50% dimethylformamide, loss of 21% activity at 90°C after 1 h Saccharolobus solfataricus
Ethanol no loss of activity after 24 h at room temperature in presence of 50% ethanol, loss of 88% activity at 90°C after 1 h Saccharolobus solfataricus
Ethanol 50%, 24 h at room temperature, no loss of activity Saccharolobus solfataricus
guanidine-HCl 4 M, 24 h at room temperature, no loss of activity Saccharolobus solfataricus
Methanol no loss of activity after 24 h at room temperature in presence of 50% methanol, loss of 80% activity at 90°C after 1 h Saccharolobus solfataricus
Methanol 50%, 24 h at room temperature, no loss of activity Saccharolobus solfataricus
N,N-dimethylformamide 50%, 24 h at room temperature, no loss of activity Saccharolobus solfataricus
SDS 0.075%, 24 h at room temperature, no loss of activity. After 60 min of incubation in 2% SDS the enzyme remains fully active at 70°C and retains 20% residual activity at 90°C. Complete inactivation after 5 min at 100°C in 0.5% SDS Saccharolobus solfataricus
tetrahydrofuran loss of 76% activity at 70°C after 30 min, complete loss of activity after 1 h at 70°C Saccharolobus solfataricus
Triton X-100 9 M, 24 h at room temperature, no loss of activity Saccharolobus solfataricus
urea 9 M, 24 h at room temperature, no loss of activity Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-
Saccharolobus solfataricus P50389
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.116
-
-
 Saccharolobus solfataricus
2.12
-
 purified enzyme Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
-20°C, 10 mM Tris-HC1, pH 7.4, containing 1 mM EDTA, stable for at least 1 year Saccharolobus solfataricus
-20°C, purified enzyme, Tris-HCl 10 mM, pH 7.4, 1 mM EDTA, stable at least 1 year Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-methylthioadenosine + phosphate
-
 Saccharolobus solfataricus adenine + 5-methylthio-D-ribose 1-phosphate
-
 ?
adenosine + phosphate
-
 Saccharolobus solfataricus adenine + D-ribose 1-phosphate
-
 ?
guanosine + phosphate
-
 Saccharolobus solfataricus guanine + D-ribose 1-phosphate
-
 ?
inosine + phosphate
-
 Saccharolobus solfataricus hypoxanthine + D-ribose 1-phosphate
-
 ?
inosine + phosphate most effective substrate Saccharolobus solfataricus hypoxanthine + D-ribose 1-phosphate
-
 ?
additional information substrate specificity Saccharolobus solfataricus ?
-
 ?
additional information no activity with S-adenosyl-L-methionine Saccharolobus solfataricus ?
-
 ?
additional information no activity with S-adenosylhomocysteine Saccharolobus solfataricus ?
-
 ?
additional information involved in the catabolism of 5'-methylthioadenosine, adenosine, guanosine and inosine Saccharolobus solfataricus ?
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 27000, SDS-PAGE Saccharolobus solfataricus
More secondary structure by circular dichroism spectra Saccharolobus solfataricus
More presence of 6 disulfide bonds, organisation in 2 trimers Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
5'-methylthioadenosine phosphorylase
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Saccharolobus solfataricus
120
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 150
-
 Saccharolobus solfataricus
90 130 90°C: about 50% of maximal activity, 130°C: about 70% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 stable for at least 2 h Saccharolobus solfataricus
120
-
 2 h, stable Saccharolobus solfataricus
130
-
 half-life: 15 min Saccharolobus solfataricus
130
-
 half-inactivation time: 15 min Saccharolobus solfataricus
132
-
 10 min, melting temperature Saccharolobus solfataricus
140
-
 half-life: 5 min Saccharolobus solfataricus
140
-
 half-inactivation time: 5 min Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 5.7 Saccharolobus solfataricus
7.4
-
-
 Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
 Saccharolobus solfataricus

pI Value

Organism Comment pI Value Maximum pI Value
Saccharolobus solfataricus isoelectric focusing on polyacrylamide gel in the pH ranges 3-10 and 4.5-6.0
-
 5.7