Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21 | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
C259S/C261S | mutant enzyme shows thermophilic and thermostable features significantly lower than those of the wild-type enzyme | Saccharolobus solfataricus |
C262S | mutant enzyme shows thermophilic and thermostable features significantly lower than those of the wild-type enzyme | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0254 | - |
adenosine | pH 7.4, 70°C | Saccharolobus solfataricus | |
0.084 | - |
Inosine | pH 7.4, 70°C | Saccharolobus solfataricus | |
0.1136 | - |
guanosine | pH 7.4, 70°C | Saccharolobus solfataricus | |
0.154 | - |
5'-methylthioadenosine | pH 7.4, 70°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
6 * 30000, SDS-PAGE | Saccharolobus solfataricus |
180000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5'-methylthioadenosine + phosphate | - |
Saccharolobus solfataricus | adenine + 5-methylthio-D-ribose 1-phosphate | - |
? | |
adenosine + phosphate | - |
Saccharolobus solfataricus | adenine + D-ribose 1-phosphate | - |
? | |
guanosine + phosphate | - |
Saccharolobus solfataricus | guanine + D-ribose 1-phosphate | - |
? | |
inosine + phosphate | - |
Saccharolobus solfataricus | hypoxanthine + D-ribose 1-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 30000, SDS-PAGE | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
5'-deoxy-5'-methylthioadenosine phosphorylase II | - |
Saccharolobus solfataricus |
SsMTAPII | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
120 | - |
wild-type enzyme | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | 140 | 95°C: about 50% of maximal activity, 140°C: about 45% of maximal activity, wild-type enzyme | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
1 h, wild-type enzyme is completely stable, mutant enzyme C262S loses 32% of its activity, mutant enzyme c259S/C261A loses 63% of its activity | Saccharolobus solfataricus |
102 | - |
apparent Tm, mutant enzyme C259S/C261S | Saccharolobus solfataricus |
106 | - |
apparent Tm, mutant enzyme C262S | Saccharolobus solfataricus |
112 | - |
apparent Tm, wild-type enzyme | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.4 | - |
5'-methylthioadenosine | pH 7.4, 70°C | Saccharolobus solfataricus | |
29.6 | - |
guanosine | pH 7.4, 70°C | Saccharolobus solfataricus | |
43.6 | - |
adenosine | pH 7.4, 70°C | Saccharolobus solfataricus | |
132 | - |
Inosine | pH 7.4, 70°C | Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | calculation from nucleotide sequence | - |
6.54 |