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2.7.1.40: pyruvate kinase

This is an abbreviated version!
For detailed information about pyruvate kinase, go to the full flat file.

Word Map on EC 2.7.1.40

Reaction

ATP
+
pyruvate
=
ADP
 +
phosphoenolpyruvate

Synonyms

ATP/pyruvate O'-phosphotransferase, ATP:pyruvate 2-O-phosphotransferase, CPK, CPK1, cPK2, cPK3, cPK4, cPK5, CTHBP, cytosolic pyruvate kinase, cytosolic thyroid hormone binding protein, EHI_098420, EhPK, EhPyk, erythroid (R-type) pyruvate kinase, fluorokinase, hL-PYK, hLPYK, hPKM2, K+-dependent PK, K+-independent PK, kinase, fluoro- (phosphorylating), kinase, pyruvate (phosphorylating), L-PK , L-PYK, liver PK, liver pyruvate kinase, M1-PK, M1-PYK, M2 pyruvate kinase, M2 type phosphoenolpyruvate kinase, M2-PK, M2-pyruvate kinase, M2-type pyruvate kinase, M2PK, mPKM2, MRSA PK, muscle PK, NCgl2008, NCgl2809, PAE0819, PfPYK, pfPyrK, phosphoenol transphosphorylase, phosphoenolpyruvate kinase, phosphoenolpyruvate: ADP phosphotransferase, PK-alphabeta1, PK-alphabeta2, PK-M1, PK-M2, PK-R, PK-S, PK1, PK3, PKC, PKC1, PKL, Pklr, PKM1, PKM2, PKp, ProTalphaK, PYK, PYK-I, Pyk1, Pyk2, PykA, PykF, PyKII, pyruvate kinase, pyruvate kinase 1, pyruvate kinase isoenzyme M2, pyruvate kinase isoform M2, pyruvate kinase isoforms 2, pyruvate kinase isozyme M1, pyruvate kinase isozyme type M2, pyruvate kinase M1, pyruvate kinase M2, pyruvate kinase M2 isoform, pyruvate kinase muscle isoform 2, pyruvate kinase muscle isozyme, pyruvate kinase type M2, pyruvate kinase type-II, pyruvate phosphotransferase, pyruvic kinase, R-type pyruvate kinase, R-type/L-type pyruvate kinase, red cell/liver pyruvate kinase, rM1-PYK, RMPK, RPK, Rv1617, SSO0981, TbrPYK, TCIL3000_10_12020, TcoPYK, THBP1, TuM2-PK, tumor cell specific pyruvate kinase isozyme M2, tumour M2-PK, tumour M2-pyruvate kinase, VcIIPK, VcIPK, VC_0485, VC_2008, VEG17, vegetative protein 17, vesicle-associated pyruvate kinase, YPK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.40 pyruvate kinase

Storage Stability

Storage Stability on EC 2.7.1.40 - pyruvate kinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, in 20 mM Tris-HCl, pH 6.8, 1 mM MgCl2, 0.1 mM dithiothreitol, 2 months
-
-20°C, in 10 mM Tris-HCl or sodium phosphate buffer, pH 7.5, 50% v/v glycerol, 18 h
-
-20°C, in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, at least 2 years
-
-20°C, in 50 mM phosphate buffer, pH 7, 100 mM KCl, 2 mM phosphoenolpyruvate, 0.1% 2-mercaptoethanol, 50% v/v glycerol, at least 6 months
-
-20°C, in potassium phosphate buffer, pH 7, MgCl2, phosphoenolpyruvate and glycerol, 5 months
-
-20°C, in the absence of substrates, 70% loss of activity within 1 month, phosphoenolpyruvate reduces this loss somewhat, ADP increases it
-
-20°C, with 50% (v/v) glycerol, 72 h, no loss of activity
-
-70°C, enzyme precipitate in ammonium sulfate solution, several years
-
-80°C, frozen with liquid N2, several months
-80°C, in the absence of substrates, 95% loss of activity within 1 month, phosphoenolpyruvate reduces this loss somewhat, ADP increases it
-
0-4°C, in 10 mM Tris, pH 7.5, 1 mM EDTA, 2 mM 2-mercaptoethanol, 1-10 mg isozyme PK I/ml, up to 9 months, in 10 mM Tris, 1 mM EDTA, 2 mM 2-mercaptoethanol, 0.15 M KCl, pH 8, 0.5-1 mg isozyme PK II/ml, up to 6 months
-
2-4°C, in 90% saturated ammonium sulfate, at least 1 month
-
25°C, in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, inactivation within a few days
-
30°C, 100 mM KH2PO4 with 100 mM Mg2+, 72 h, 70% loss of activity
-
37°C, 100 mM KH2PO4 with 100 mM Mg2+, 72 h, 95% loss of activity
-
4°C, 20 mM Tris-HCl, pH 7.0, and 30% glycerol, 1 week, remains stable
-
4°C, ammonium sulfate preparation, at least 5 months
-
4°C, in 10 mM Tris-HCl or sodium phosphate buffer, pH 7.5, 50% v/v glycerol, 18 h
-
4°C, in 20 mM Tris-HCl, pH 7.0, 30% glycerol, 200 mM KCl, stable for 1 week
4°C, in 25 mM imidazole-HCl buffer, pH 7.1, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 M sucrose and 0.04% NaN3, concentrated by dialysis against solid polyethylene glycol, at least 4 weeks, isozyme PK-aerobic
Busycotypus canaliculatum
-
4°C, in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, at least 2 years, at pH-values below 5, inactivation within a few days
-
4°C, in 40 mM potassium phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 3 months
-
4°C, in 50 mM Tris-HCl buffer, isozyme I up to 10 days, isozyme II 4 days
-
4°C, in the absence of substrates, t1/2: 1 month
-
4°C, lyophilized lyzed trypanosomes, 3 h after resuspension, 20% loss of activity, storage in aqueous solution leads to a decrease in activity and cooperative nature of phosphoenolpyruvate-enzyme interaction
-
5°C, isozyme PKp in 50 mM phosphate buffer, pH 7, 50%, v/v, glycerol, 2 mM dithiothreitol, several months and isozyme PKc in 50 mM phosphate buffer, pH 7, at least 1 week
-
8°C, isozyme PKI in the absence of substrates, less than 20% loss of activity within 1 month and isozyme PKII with t1/2 of 5 days, 25% loss within 1 month in the presence of phosphoenolpyruvate or 60% loss of activity in the presence of ADP
-
EDTA, 2-mercaptoethanol, dithiothreitol, glycerol and KCl stabilize, being more effective at refrigerated conditions than at room temperature
-
frozen, in 50 mM Tris-HCl buffer, isozyme I at least 9 months, isozyme II 4 months
-
glycerol stabilizes during storage
-
glycerol, 25% w/v, or 0.1 mM dithiothreitol stabilizes, more effective under refrigerated conditions than at room temperature, KCl, MgSO4, EDTA, phosphoenolpyruvate, ADP or (NH4)2SO4 decreases stability at either 4°C or 17°C
-
K+ stabilize during storage, NH4+ or Na+ less efficiently
-