Activating Compound | Comment | Organism | Structure |
---|---|---|---|
asparagine | activation | Busycotypus canaliculatum | |
D-fructose 1,6-diphosphate | activation | Busycotypus canaliculatum | |
D-fructose 1,6-diphosphate | stimulates aerobic isozyme more strongly than anoxic isozyme | Busycotypus canaliculatum | |
D-fructose 1,6-diphosphate | synergism with Asp, PK-aerobic, not PK-anoxic | Busycotypus canaliculatum | |
additional information | interacting effects of various activators and inhibitors | Busycotypus canaliculatum |
General Stability | Organism |
---|---|
0.5 M sucrose and 0.1 M KCl stabilize | Busycotypus canaliculatum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | activators restore | Busycotypus canaliculatum | |
ATP | MgATP2- | Busycotypus canaliculatum | |
MgATP2- | activators restore activity | Busycotypus canaliculatum | |
additional information | interacting effects of various activators and inhibitors | Busycotypus canaliculatum | |
phosphate | activators restore | Busycotypus canaliculatum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters | Busycotypus canaliculatum | |
0.04 | 0.063 | phosphoenolpyruvate | in the absence of fructose 1,6-diphosphate | Busycotypus canaliculatum | |
0.04 | 0.063 | phosphoenolpyruvate | in the presence of fructose 1,6-diphosphate | Busycotypus canaliculatum | |
0.04 | 0.063 | phosphoenolpyruvate | pH 7.0, 20°C | Busycotypus canaliculatum | |
0.2 | 0.35 | ADP | pH 7.0, 20°C | Busycotypus canaliculatum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | - |
Busycotypus canaliculatum | |
Mg2+ | - |
Busycotypus canaliculatum | |
Mn2+ | can partially replace Mg2+ | Busycotypus canaliculatum | |
additional information | interacting effects of various activators and inhibitors | Busycotypus canaliculatum | |
NH4+ | requirement | Busycotypus canaliculatum | |
NH4+ | can replace K+ | Busycotypus canaliculatum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
71000 | - |
4 * 71000, PK-aerobic, SDS-PAGE | Busycotypus canaliculatum |
72600 | - |
4 * 72600, PK-anoxic, SDS-PAGE | Busycotypus canaliculatum |
286000 | - |
PK-aerobic, gel filtration | Busycotypus canaliculatum |
299800 | - |
PK-anoxic, gel filtration | Busycotypus canaliculatum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Busycotypus canaliculatum | - |
channelled whelk, gastropod mollusc | - |
Purification (Comment) | Organism |
---|---|
aerobic and anoxic variants, i.e. isozymes PK-aerobic and PK-anoxic | Busycotypus canaliculatum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hepatopancreas | - |
Busycotypus canaliculatum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Busycotypus canaliculatum |
Storage Stability | Organism |
---|---|
4°C, in 25 mM imidazole-HCl buffer, pH 7.1, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 M sucrose and 0.04% NaN3, concentrated by dialysis against solid polyethylene glycol, at least 4 weeks, isozyme PK-aerobic | Busycotypus canaliculatum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + phosphoenolpyruvate | - |
Busycotypus canaliculatum | ATP + pyruvate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 72600, PK-anoxic, SDS-PAGE | Busycotypus canaliculatum |
tetramer | 4 * 71000, PK-aerobic, SDS-PAGE | Busycotypus canaliculatum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
broad | Busycotypus canaliculatum |