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Literature summary for 2.7.1.40 extracted from

  • Plaxton, W.C.; Storey, K.B.
    Purification and properties of aerobic and anoxic forms of pyruvate kinase from the hepatopancreas of the channelled whelk, Busycotypus canaliculatum (1985), Arch. Biochem. Biophys., 243, 195-205.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
asparagine activation Busycotypus canaliculatum
D-fructose 1,6-diphosphate activation Busycotypus canaliculatum
D-fructose 1,6-diphosphate stimulates aerobic isozyme more strongly than anoxic isozyme Busycotypus canaliculatum
D-fructose 1,6-diphosphate synergism with Asp, PK-aerobic, not PK-anoxic Busycotypus canaliculatum
additional information interacting effects of various activators and inhibitors Busycotypus canaliculatum

General Stability

General Stability Organism
0.5 M sucrose and 0.1 M KCl stabilize Busycotypus canaliculatum

Inhibitors

Inhibitors Comment Organism Structure
ADP activators restore Busycotypus canaliculatum
ATP MgATP2- Busycotypus canaliculatum
MgATP2- activators restore activity Busycotypus canaliculatum
additional information interacting effects of various activators and inhibitors Busycotypus canaliculatum
phosphate activators restore Busycotypus canaliculatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic parameters Busycotypus canaliculatum
0.04 0.063 phosphoenolpyruvate in the absence of fructose 1,6-diphosphate Busycotypus canaliculatum
0.04 0.063 phosphoenolpyruvate in the presence of fructose 1,6-diphosphate Busycotypus canaliculatum
0.04 0.063 phosphoenolpyruvate pH 7.0, 20°C Busycotypus canaliculatum
0.2 0.35 ADP pH 7.0, 20°C Busycotypus canaliculatum

Metals/Ions

Metals/Ions Comment Organism Structure
K+
-
Busycotypus canaliculatum
Mg2+
-
Busycotypus canaliculatum
Mn2+ can partially replace Mg2+ Busycotypus canaliculatum
additional information interacting effects of various activators and inhibitors Busycotypus canaliculatum
NH4+ requirement Busycotypus canaliculatum
NH4+ can replace K+ Busycotypus canaliculatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
4 * 71000, PK-aerobic, SDS-PAGE Busycotypus canaliculatum
72600
-
4 * 72600, PK-anoxic, SDS-PAGE Busycotypus canaliculatum
286000
-
PK-aerobic, gel filtration Busycotypus canaliculatum
299800
-
PK-anoxic, gel filtration Busycotypus canaliculatum

Organism

Organism UniProt Comment Textmining
Busycotypus canaliculatum
-
channelled whelk, gastropod mollusc
-

Purification (Commentary)

Purification (Comment) Organism
aerobic and anoxic variants, i.e. isozymes PK-aerobic and PK-anoxic Busycotypus canaliculatum

Source Tissue

Source Tissue Comment Organism Textmining
hepatopancreas
-
Busycotypus canaliculatum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Busycotypus canaliculatum

Storage Stability

Storage Stability Organism
4°C, in 25 mM imidazole-HCl buffer, pH 7.1, 10 mM 2-mercaptoethanol, 1 mM EDTA, 0.5 M sucrose and 0.04% NaN3, concentrated by dialysis against solid polyethylene glycol, at least 4 weeks, isozyme PK-aerobic Busycotypus canaliculatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Busycotypus canaliculatum ATP + pyruvate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 72600, PK-anoxic, SDS-PAGE Busycotypus canaliculatum
tetramer 4 * 71000, PK-aerobic, SDS-PAGE Busycotypus canaliculatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
broad Busycotypus canaliculatum