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Literature summary for 2.7.1.40 extracted from

  • Plaxton, W.C.
    Purification of pyruvate kinase from germinating castor bean endosperm (1988), Plant Physiol., 86, 1064-1069.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol required for optimal activity Ricinus communis

General Stability

General Stability Organism
even in the presence of glycerol or ethylene glycol, Mg2+ and dithioerythritol, at protein concentrations below 0.4 mg/ml, the enzyme is very unstable during extraction and purification Ricinus communis

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Ricinus communis 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ requirement Ricinus communis
Mg2+ requirement Ricinus communis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
x * 56000 + x * 57000, SDS-PAGE Ricinus communis
57000
-
x * 56000 + x * 57000, SDS-PAGE Ricinus communis
240000
-
gel filtration Ricinus communis

Organism

Organism UniProt Comment Textmining
Ricinus communis
-
cv. Hale
-

Purification (Commentary)

Purification (Comment) Organism
-
Ricinus communis

Source Tissue

Source Tissue Comment Organism Textmining
endosperm germinating (from 5 days old seedlings) Ricinus communis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
203
-
pH 6.9, 30°C Ricinus communis

Storage Stability

Storage Stability Organism
-80°C, frozen with liquid N2, several months Ricinus communis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate
-
Ricinus communis ATP + pyruvate
-
?

Subunits

Subunits Comment Organism
tetramer x * 56000 + x * 57000, SDS-PAGE Ricinus communis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Ricinus communis