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2.7.1.40: pyruvate kinase

This is an abbreviated version!
For detailed information about pyruvate kinase, go to the full flat file.

Word Map on EC 2.7.1.40

Reaction

ATP
+
pyruvate
=
ADP
+
phosphoenolpyruvate

Synonyms

ATP/pyruvate O'-phosphotransferase, ATP:pyruvate 2-O-phosphotransferase, CPK, CPK1, cPK2, cPK3, cPK4, cPK5, CTHBP, cytosolic pyruvate kinase, cytosolic thyroid hormone binding protein, EHI_098420, EhPK, EhPyk, erythroid (R-type) pyruvate kinase, fluorokinase, hL-PYK, hLPYK, hPKM2, K+-dependent PK, K+-independent PK, kinase, fluoro- (phosphorylating), kinase, pyruvate (phosphorylating), L-PK , L-PYK, liver PK, liver pyruvate kinase, M1-PK, M1-PYK, M2 pyruvate kinase, M2 type phosphoenolpyruvate kinase, M2-PK, M2-pyruvate kinase, M2-type pyruvate kinase, M2PK, mPKM2, MRSA PK, muscle PK, NCgl2008, NCgl2809, PAE0819, PfPYK, pfPyrK, phosphoenol transphosphorylase, phosphoenolpyruvate kinase, phosphoenolpyruvate: ADP phosphotransferase, PK-alphabeta1, PK-alphabeta2, PK-M1, PK-M2, PK-R, PK-S, PK1, PK3, PKC, PKC1, PKL, Pklr, PKM1, PKM2, PKp, ProTalphaK, PYK, PYK-I, Pyk1, Pyk2, PykA, PykF, PyKII, pyruvate kinase, pyruvate kinase 1, pyruvate kinase isoenzyme M2, pyruvate kinase isoform M2, pyruvate kinase isoforms 2, pyruvate kinase isozyme M1, pyruvate kinase isozyme type M2, pyruvate kinase M1, pyruvate kinase M2, pyruvate kinase M2 isoform, pyruvate kinase muscle isoform 2, pyruvate kinase muscle isozyme, pyruvate kinase type M2, pyruvate kinase type-II, pyruvate phosphotransferase, pyruvic kinase, R-type pyruvate kinase, R-type/L-type pyruvate kinase, red cell/liver pyruvate kinase, rM1-PYK, RMPK, RPK, Rv1617, SSO0981, TbrPYK, TCIL3000_10_12020, TcoPYK, THBP1, TuM2-PK, tumor cell specific pyruvate kinase isozyme M2, tumour M2-PK, tumour M2-pyruvate kinase, VcIIPK, VcIPK, VC_0485, VC_2008, VEG17, vegetative protein 17, vesicle-associated pyruvate kinase, YPK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.40 pyruvate kinase

General Stability

General Stability on EC 2.7.1.40 - pyruvate kinase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
0.1 M KCl stabilizes enzyme in dilute solutions
-
0.5 M sucrose and 0.1 M KCl stabilize
Busycotypus canaliculatum
-
25 mM MES and 25 mM Bis-Tris propane as the assay buffer
-
at either pH value, the enzyme displays equivalent activity in 50 mM HEPES or a mixture of
-
binding of frucctose 2,6-bisphophate plays the most important role in stabilizing the LmPYK tetramer in solution
cytosolic isozyme is much more stable than plastid isozyme, dithiothreitol plus glycerol stabilize plastid isozyme PKp during purification, isozyme PKp requires dithiothreitol or 2-mercaptoethanol during purification, cytosolic isozyme PKc is stable to dilution into 50 mM N-tris[hydroxymethyl]methyl-2-aminoethane-sulfonic acid buffer, pH 7.9
-
dialysis against 10 mM sodium phosphate or Tris-HCl, pH 7.5, for 18 h at 4°C, inactivates
-
dialysis for 20 h against 40 mM Tris-HCl, Tris-maleate or morpholinepropanesulfonic acid-NaOH buffer, pH 7, inactivates, but stable to dialysis against phosphate buffer, pH 7
-
even in the presence of glycerol or ethylene glycol, Mg2+ and dithioerythritol, at protein concentrations below 0.4 mg/ml, the enzyme is very unstable during extraction and purification
-
freezing inactivates
-
fructose 1,6-diphosphate and bovine serum albumin stabilize
-
glycerol stabilizes during purification
-
glycerol, 50%, stabilizes
-
highly unstable at less than 3 M sodium or potassium chloride, cannot be renatured
-
labile enzyme is stabilized in the active state throughout purification by avoiding extensive dilution to less than 40 mg protein/ml, and by inclusion of a stabilizing ligand mixture of phosphoenolpyruvate, phosphate and Mg2+ in supporting buffers if dilution is unavoidable
-
MgATP2-, NAD+, NADH, 50% glycerol or sucrose partially stabilizes
-
optimal stability at pH 6.5 and room temperature
-
phosphate buffer, MgSO4, 2-mercaptoethanol, fructose 1,6-diphosphate stabilize during purification at room temperature
-
phosphate restores activity of dialyzed, partially purified, not fully purified, enzyme preparations
-
the enzyme activity remains stable after one freeze-thaw cycle
-
the enzyme cannot be solubilized in detergents, but trypsin without loss of activity
-
unstable in aqueous solution, thiol protecting agents and glycerol stabilize
-