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Literature summary for 2.7.1.40 extracted from

  • Flynn, I.W.; Bowman, I.B.R.
    Purification and characterization of pyruvate kinase from Trypanosoma brucei (1980), Arch. Biochem. Biophys., 200, 401-409.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cysteine activation, can replace dithiothreitol Trypanosoma brucei
D-fructose 1,6-diphosphate activation Trypanosoma brucei
D-fructose 1,6-diphosphate kinetics Trypanosoma brucei
D-glucose 6-phosphate
-
Trypanosoma brucei
dithiothreitol activation Trypanosoma brucei
GSH activation, can replace dithiothreittol Trypanosoma brucei

General Stability

General Stability Organism
glycerol stabilizes during purification Trypanosoma brucei
unstable in aqueous solution, thiol protecting agents and glycerol stabilize Trypanosoma brucei

Inhibitors

Inhibitors Comment Organism Structure
K+ above 100 mM, activates below Trypanosoma brucei
Na+ above 100 mM, activates below Trypanosoma brucei
NH4+ above 100 mM, activates below Trypanosoma brucei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.082 0.4 ADP pH 7.4, 25°C Trypanosoma brucei
0.098 0.1 GDP pH 7.4, 25°C Trypanosoma brucei
0.41
-
UDP pH 7.4, 25°C Trypanosoma brucei
2.53
-
CDP pH 7.4, 25°C Trypanosoma brucei

Metals/Ions

Metals/Ions Comment Organism Structure
K+ requirement Trypanosoma brucei
K+ inhibits above 100 mM Trypanosoma brucei
Mg2+ requirement Trypanosoma brucei
Mn2+ requirement Trypanosoma brucei
monovalent cation requirement Trypanosoma brucei
Na+ inhibits above 100 mM Trypanosoma brucei
NH4+ requirement Trypanosoma brucei
NH4+ can replace K+ Trypanosoma brucei
NH4+ inhibits above 100 mM Trypanosoma brucei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
gel filtration Trypanosoma brucei

Organism

Organism UniProt Comment Textmining
Trypanosoma brucei
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Trypanosoma brucei

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Trypanosoma brucei
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Trypanosoma brucei

Storage Stability

Storage Stability Organism
4°C, lyophilized lyzed trypanosomes, 3 h after resuspension, 20% loss of activity, storage in aqueous solution leads to a decrease in activity and cooperative nature of phosphoenolpyruvate-enzyme interaction Trypanosoma brucei
glycerol, 25% w/v, or 0.1 mM dithiothreitol stabilizes, more effective under refrigerated conditions than at room temperature, KCl, MgSO4, EDTA, phosphoenolpyruvate, ADP or (NH4)2SO4 decreases stability at either 4°C or 17°C Trypanosoma brucei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Trypanosoma brucei ATP + pyruvate
-
?
ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Trypanosoma brucei ATP + pyruvate
-
?
CDP + phosphoenolpyruvate less effective than ADP Trypanosoma brucei CTP + pyruvate
-
?
GDP + phosphoenolpyruvate less effective than ADP Trypanosoma brucei GTP + pyruvate
-
?
additional information preferred: purine nucleotides Trypanosoma brucei ?
-
?
UDP + phosphoenolpyruvate less effective than ADP Trypanosoma brucei UTP + pyruvate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Trypanosoma brucei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Trypanosoma brucei