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2.7.1.40: pyruvate kinase

This is an abbreviated version!
For detailed information about pyruvate kinase, go to the full flat file.

Word Map on EC 2.7.1.40

Reaction

ATP
+
pyruvate
=
ADP
 +
phosphoenolpyruvate

Synonyms

ATP/pyruvate O'-phosphotransferase, ATP:pyruvate 2-O-phosphotransferase, CPK, CPK1, cPK2, cPK3, cPK4, cPK5, CTHBP, cytosolic pyruvate kinase, cytosolic thyroid hormone binding protein, EHI_098420, EhPK, EhPyk, erythroid (R-type) pyruvate kinase, fluorokinase, hL-PYK, hLPYK, hPKM2, K+-dependent PK, K+-independent PK, kinase, fluoro- (phosphorylating), kinase, pyruvate (phosphorylating), L-PK , L-PYK, liver PK, liver pyruvate kinase, M1-PK, M1-PYK, M2 pyruvate kinase, M2 type phosphoenolpyruvate kinase, M2-PK, M2-pyruvate kinase, M2-type pyruvate kinase, M2PK, mPKM2, MRSA PK, muscle PK, NCgl2008, NCgl2809, PAE0819, PfPYK, pfPyrK, phosphoenol transphosphorylase, phosphoenolpyruvate kinase, phosphoenolpyruvate: ADP phosphotransferase, PK-alphabeta1, PK-alphabeta2, PK-M1, PK-M2, PK-R, PK-S, PK1, PK3, PKC, PKC1, PKL, Pklr, PKM1, PKM2, PKp, ProTalphaK, PYK, PYK-I, Pyk1, Pyk2, PykA, PykF, PyKII, pyruvate kinase, pyruvate kinase 1, pyruvate kinase isoenzyme M2, pyruvate kinase isoform M2, pyruvate kinase isoforms 2, pyruvate kinase isozyme M1, pyruvate kinase isozyme type M2, pyruvate kinase M1, pyruvate kinase M2, pyruvate kinase M2 isoform, pyruvate kinase muscle isoform 2, pyruvate kinase muscle isozyme, pyruvate kinase type M2, pyruvate kinase type-II, pyruvate phosphotransferase, pyruvic kinase, R-type pyruvate kinase, R-type/L-type pyruvate kinase, red cell/liver pyruvate kinase, rM1-PYK, RMPK, RPK, Rv1617, SSO0981, TbrPYK, TCIL3000_10_12020, TcoPYK, THBP1, TuM2-PK, tumor cell specific pyruvate kinase isozyme M2, tumour M2-PK, tumour M2-pyruvate kinase, VcIIPK, VcIPK, VC_0485, VC_2008, VEG17, vegetative protein 17, vesicle-associated pyruvate kinase, YPK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.40 pyruvate kinase

Specific Activity

Specific Activity on EC 2.7.1.40 - pyruvate kinase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.001
-
below, wild-type strain DSM133, pH 7.5, 55°C
0.004
-
pH 7.5, 37°C, activity in undialyzed cell extracts
0.02
-
in the absence of K+
0.028
-
50°C, pH not specified in the publication, enzyme from lactate-grown cells
0.039
-
clarified extract
0.12
-
sperm homogenate
0.13
-
50°C, pH not specified in the publication, enzyme from starch-grown cells
0.14
-
mutant strain YD01, pH 7.5, 55°C
0.49 - 1.32
purified native liver enzyme, pH 7.2, 22°C
0.52
-
mutant strain DS8, pH 7.5, 55°C
0.84
-
mutant strain YD02, pH 7.5, 55°C
1.33
-
crude extract, at pH 7.0 and 37°C
1.64
purified recombinant enzyme, pH 7.0, 35°C
108
-
purified recombinant PykF, pH 7.5, 30°C
11.8 - 14.5
purified native muscle enzyme, pH 7.2, 22°C
116.7
-
after 972fold purification
1341
-
pH 7.1, 37°C
156.9
mutant K453L, pH 7.2, 25°C
1685
-
isozyme PK5, pH 6.5, 30°C
178.1
wild-type, pH 7.2, 25°C
191
-
pH 7.4, 22°C
201
-
pH 7.5, 60°C
203
-
pH 6.9, 30°C
210
-
pH 7.1, 30°C
218
-
isozyme PKp
233
-
25°C, pH 7.0, M-type isozyme from neck muscle
307
-
pH 7.4, 37°C, R-type isozyme
330
-
pH 8.0, 30°C, erythrocyte enzyme
34.76
-
after 26.14fold purification, at pH 7.0 and 37°C
340
-
pH 6.2, 30°C
368
Busycotypus canaliculatum
-
isozyme PK-aerobic, pH 7.0, 20°C
390
-
purified enzyme, pH 7.0, 37°C
397.5
-
K-type isozyme
420
-
liver enzyme, pH 8.0, 30°C
5.31
purified recombinant His-tagged enzyme, pH 7.6, 37°C
520
-
M2-type isozyme, 25°C, pH 7.4
59
Musa cavendishii
-
pH 6.9, 25°C
593
-
pH 7.4, 37°C
68.2
mutant H480Q, pH 7.2, 25°C
690.7
-
partially purified cytosolic isozyme, pH 7.0, 25°C
73.1
-
after 1874fold purification
770
-
M2-type isozyme, 37°C, pH 7.4
780
-
M1-type isozyme, 37°C, pH 7.4
85
-
purified recombinant PykA, pH 7.5, 30°C
98
mutant K453L/H480Q, pH 7.2, 25°C
additional information