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Literature summary for 2.7.1.40 extracted from

  • Sakai, H.; Suzuki, K.; Imahori, K.
    Purification and properties of pyruvate kinase from Bacillus stearothermophilus (1986), J. Biochem., 99, 1157-1167.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
3-phosphoglycerate slight activation Geobacillus stearothermophilus
D-fructose 1,6-diphosphate not Geobacillus stearothermophilus
D-glucose 1-phosphate not Geobacillus stearothermophilus
D-glucose 6-phosphate activation Geobacillus stearothermophilus
D-ribose 5-phosphate activation Geobacillus stearothermophilus

Inhibitors

Inhibitors Comment Organism Structure
ADP at high concentrations; substrate inhibition Geobacillus stearothermophilus
Ca2+
-
Geobacillus stearothermophilus
Cu2+
-
Geobacillus stearothermophilus
Mg2+ free form Geobacillus stearothermophilus
Ni2+
-
Geobacillus stearothermophilus
phosphate
-
Geobacillus stearothermophilus
Sr2+
-
Geobacillus stearothermophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ not Geobacillus stearothermophilus
Co2+ can replace Mg2+ Geobacillus stearothermophilus
Co2+ requirement Geobacillus stearothermophilus
K+ requirement Geobacillus stearothermophilus
Mg2+ requirement Geobacillus stearothermophilus
Mn2+ requirement Geobacillus stearothermophilus
Mn2+ can partially replace Mg2+ Geobacillus stearothermophilus
additional information no activation by Sr2+ Geobacillus stearothermophilus
additional information no activation by Cu2+, Ni2+ Geobacillus stearothermophilus
Na+ can poorly replace K+ Geobacillus stearothermophilus
NH4+ requirement Geobacillus stearothermophilus
NH4+ can replace K+ Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid composition Geobacillus stearothermophilus
242000
-
sedimentation analysis Geobacillus stearothermophilus
250000
-
gel filtration Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information no glycoprotein Geobacillus stearothermophilus

Purification (Commentary)

Purification (Comment) Organism
no isozymes Geobacillus stearothermophilus

Reaction

Reaction Comment Organism Reaction ID
ATP + pyruvate = ADP + phosphoenolpyruvate allosteric enzyme: homotropic Geobacillus stearothermophilus

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Geobacillus stearothermophilus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
333
-
pH 7.2, 30°C Geobacillus stearothermophilus

Storage Stability

Storage Stability Organism
-20°C, in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, at least 2 years Geobacillus stearothermophilus
25°C, in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, inactivation within a few days Geobacillus stearothermophilus
4°C, in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, at least 2 years, at pH-values below 5, inactivation within a few days Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + phosphoenolpyruvate no positive cooperativity for ADP Geobacillus stearothermophilus ATP + pyruvate
-
?
ADP + phosphoenolpyruvate best nucleoside diphosphate substrate Geobacillus stearothermophilus ATP + pyruvate
-
?
ADP + phosphoenolpyruvate positive cooperativity for phosphoenolpyruvate Geobacillus stearothermophilus ATP + pyruvate
-
?
CDP + phosphoenolpyruvate poor substrate Geobacillus stearothermophilus CTP + pyruvate
-
?
CDP + phosphoenolpyruvate less effective than ADP Geobacillus stearothermophilus CTP + pyruvate
-
?
epsilon-ADP + phosphoenolpyruvate poor substrate Geobacillus stearothermophilus ?
-
?
GDP + phosphoenolpyruvate less effective than ADP Geobacillus stearothermophilus GTP + pyruvate
-
?
IDP + phosphoenolpyruvate less effective than ADP Geobacillus stearothermophilus ITP + pyruvate
-
?
additional information broad specificity for nucleoside diphosphates Geobacillus stearothermophilus ?
-
?
additional information allosteric enzyme: homotropic Geobacillus stearothermophilus ?
-
?
UDP + phosphoenolpyruvate less effective than ADP Geobacillus stearothermophilus UTP + pyruvate
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 62000-64000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
in 25 mM Tris-HCl buffer, pH 7.5, 50 mM KCl, 5 mM MgCl2, 50% ethylene glycol, inactivation within a few days Geobacillus stearothermophilus
65
-
above, rapid inactivation, in the presence of 10 mM MgCl2: t1/2: more than 30 min, 50 mM KCl protects slightly, not phosphoenolpyruvate Geobacillus stearothermophilus
70
-
5 min, inactivation, t1/2: 1 min Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
60°C Geobacillus stearothermophilus
7.2
-
30°C Geobacillus stearothermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.4 60°C, about half-maximal activity at pH 5.5 and pH 7.4 Geobacillus stearothermophilus
6.4 8.2 30°C, about half-maximal activity at pH 6.4 and pH 8.2 Geobacillus stearothermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
below, inactivation within a few days, 4°C Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
AMP allosteric effector Geobacillus stearothermophilus
CMP activation Geobacillus stearothermophilus
CMP allosteric Geobacillus stearothermophilus
GDP activation Geobacillus stearothermophilus
GDP allosteric Geobacillus stearothermophilus
GMP activation Geobacillus stearothermophilus
GMP allosteric Geobacillus stearothermophilus