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2.7.1.20: adenosine kinase

This is an abbreviated version!
For detailed information about adenosine kinase, go to the full flat file.

Word Map on EC 2.7.1.20

Reaction

ATP
+
adenosine
=
ADP
 +
AMP

Synonyms

adenosine kinase (phosphorylating), ADK, AdK-L, AdK-S, Ado kinase, AdoK, AK, ATP:adenosine 5'-phosphotransferase, CpAK, hADK, kinase, adenosine (phosphorylating), LdAdK, MGG_06270, Rv2202c, Tb927.6.2360, TbAK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.20 adenosine kinase

Storage Stability

Storage Stability on EC 2.7.1.20 - adenosine kinase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15ºC, stable for few weeks
-
-18°C, in 20% glycerol, at least 6 months
-20°C, 2 months
-
-20°C, concentrated enzyme solution, at least 3 months
-
-20°C, in 45% sucrose, at least 1 month
-
-20°C, partially purified, 1 year
-
-20°C, purified native enzyme, 20% glycerol, stable up to 6 months
-20ºC, 10% glycerol, purified preparation
-
-25°C, 1 mg protein/ml, several months
-
-25ºC, variable losses of activity
-
-40°C, at least 1 year
-
-70°C, 0.5 mg protein/ml or more, purified preparation, at least 8 months
-
-70°C, at least 1 year
-
-70ºC, soluble protein fractions, stable
-
-75°C, pure enzyme, 20 mM Tris-HCl, pH 8.0, 15 mM 2-mercaptoethanol, stable for months
-
-80°C to -20°C, 6 months
-
-80°C, purified enzyme in 10% (v/v) glycerol, several months, no loss of activity
-
4°C, glycerol, dithiothreitol and adenosine stabilize. 20% glycerol, t1/2 20 days, 75% loss of activity within 60 days. Without glycerol, t1/2 3 days. -70°C to 4°C, 0.01 mg protein/ml, 75 to 95% loss of activity within days
-
4°C, with DTT or 2-mercaptoethanol, about 90% loss of activity, ATP or GTP does not stabilize
-
4ºC, dithiothreitol or high concentration of salt, stable. Rapid loss of activity at 4ºC with low concentration of salt, recovered when redialyzed against dithiothreitol
-