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Literature summary for 2.7.1.20 extracted from

  • Cassera, M.B.; Ho, M.C.; Merino, E.F.; Burgos, E.S.; Rinaldo-Matthis, A.; Almo, S.C.; Schramm, V.L.
    A high-affinity adenosine kinase from Anopheles gambiae (2011), Biochemistry, 50, 1885-1893.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-AI cells Anopheles gambiae

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with with P1,P4-di(adenosine-5') tetraphosphate, sitting drop vapor diffusion method, using 25% (w/v) PEG3350, 0.2 M MgCl2, 5% (v/v) 2-propanol, 25% (v/v) glycerol and 0.1 M BisTris buffer pH 5.5, at 18°C Anopheles gambiae

Inhibitors

Inhibitors Comment Organism Structure
iodotubercidin
-
 Anopheles gambiae
additional information 9-beta-D-arabinofuranosyladenine analogue of adenosine at concentrations in 150fold excess over adenosine does not affect the enzyme activity Anopheles gambiae
P1,P3-di(adenosine-5')triphosphate
-
 Anopheles gambiae
P1,P4-di(adenosine-5')tetraphosphate
-
 Anopheles gambiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000081
-
 adenosine with ATP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.00023
-
 adenosine with GTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.00063
-
 adenosine with CTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.00074
-
 adenosine with UTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.00079
-
 adenosine with TTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.0014
-
 ATP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.049
-
 CTP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.054
-
 GTP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.056
-
 UTP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.245
-
 TTP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae

Organism

Organism UniProt Comment Textmining
Anopheles gambiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, gel filtration Anopheles gambiae

Source Tissue

Source Tissue Comment Organism Textmining
adult
-
 Anopheles gambiae
-

Storage Stability

Storage Stability Organism
-80°C, purified enzyme in 10% (v/v) glycerol, several months, no loss of activity Anopheles gambiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine the enzyme is highly specific for adenosine and shows best activity with ATP as cosubstrate Anopheles gambiae ADP + AMP
-
 ?
CTP + adenosine
-
 Anopheles gambiae CDP + AMP
-
 ?
GTP + adenosine
-
 Anopheles gambiae GDP + AMP
-
 ?
TTP + adenosine
-
 Anopheles gambiae TDP + AMP
-
 ?
UTP + adenosine
-
 Anopheles gambiae UDP + AMP
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.029
-
 adenosine with CTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.035
-
 adenosine with UTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.056
-
 adenosine with TTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.061
-
 UTP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.062
-
 CTP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.16
-
 adenosine with ATP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.3
-
 TTP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.33
-
 ATP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.63
-
 adenosine with GTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
1.39
-
 GTP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000001
-
 iodotubercidin at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.00086
-
 P1,P4-di(adenosine-5')tetraphosphate at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
0.061
-
 P1,P3-di(adenosine-5')triphosphate at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.09
-
 UTP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
1.2
-
 TTP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
1.27
-
 CTP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
26
-
 GTP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
240
-
 ATP at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
2700
-
 adenosine with GTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
4600
-
 adenosine with CTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
4700
-
 adenosine with UTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
7100
-
 adenosine with TTP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae
19700
-
 adenosine with ATP as cosubstrate, at 27°C in 50 mM Tris buffer (pH 7.4) containing 5 mM MgCl2, 50 mM KCl, and 0.2 mM dithiothreitol Anopheles gambiae