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Literature summary for 2.7.1.20 extracted from
- Kinetic studies of adenosine kinase from Ehrlich ascites tumor cells (1972), J. Biol. Chem., 247, 1972-1975.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-Methylmercaptopurine riboside | and analogs | Mus musculus |  |
| 6-methylmercaptopurine riboside phosphate | product inhibition, competitive with respect to ATP and noncompetitive with respect to 6-methylmercaptopurine ribonucleoside | Mus musculus | |
| 7-beta-D-ribofuranosyl-pyrrolo-(2,3-d)-pyrimidine | competitive with respect to 6-methylmercaptopurine riboside and noncompetitive with respect to ATP | Mus musculus | |
| adenosine | competitive with respect to 6-methylmercaptopurine riboside | Mus musculus | |
| ADP | noncompetitive with respect to ATP and 6-methylmercaptopurine ribonucleoside at low concentrations of the substrates; product inhibition | Mus musculus | |
| ATP-analogs | - |
Mus musculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic study | Mus musculus | |
| 0.01 | - |
6-Methylmercaptopurine riboside | pH 6.0, 37ºC | Mus musculus | |
| 0.025 | - |
ATP | pH 6.0, 37ºC | Mus musculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate fractionation and gel filtration, 15-20fold purification | Mus musculus |
| partial | Mus musculus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + adenosine = ADP + AMP | mechanism | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, partially purified, 1 year | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 6-methylmercaptopurine riboside | - |
Mus musculus | ADP + 6-methylmercaptopurine-D-riboside 5'-phosphate | - |
? | |
| ATP + adenosine | - |
Mus musculus | ADP + AMP | - |
? | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
7-beta-D-ribofuranosyl-pyrrolo-(2,3-d)-pyrimidine | pH 6.0, 37ºC | Mus musculus | |
| 0.06 | - |
ADP | pH 6.0, 37ºC | Mus musculus | |
| 0.16 | - |
6-methylmercaptopurine riboside phosphate | pH 6.0, 37ºC | Mus musculus | |
| 0.26 | - |
adenosine | pH 6.0, 37ºC | Mus musculus | |
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Release 2023.1 (January 2023)
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