Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.7.1.20: adenosine kinase

This is an abbreviated version!
For detailed information about adenosine kinase, go to the full flat file.

Word Map on EC 2.7.1.20

Reaction

ATP
+
adenosine
=
ADP
 +
AMP

Synonyms

adenosine kinase (phosphorylating), ADK, AdK-L, AdK-S, Ado kinase, AdoK, AK, ATP:adenosine 5'-phosphotransferase, CpAK, hADK, kinase, adenosine (phosphorylating), LdAdK, MGG_06270, Rv2202c, Tb927.6.2360, TbAK

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.20 adenosine kinase

Cloned

Cloned on EC 2.7.1.20 - adenosine kinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isozymes AK-L and AK-S, DNA and amino acid sequence determination and analysis, overexpression of His-tagged proteins in Escherichia coli strain BL21(DE3)
CpAK gene codon-optimized for expression in Escherichia coli
-
DNA sequence determination and analysis, the His-tagged enzyme is expressed with or without coexpression of viral AL2 and L2 proteins in an enzyme-deficient Escherichia coli strain, enzyme expression leads to complementation of the mutant, coexpression leads to inactivation of the enzyme and no complementation, expression in yeast cells utilizing the two-hybrid system coexpressing the L2 protein of geminivirus, coexpression leads to inactivation of the enzyme
expresion in Saccharomyces cerevisiae
-
expressed in CHO, V79 and GM7S cells and several CHO mutant cell lines with reduced adenosine kinase activity
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21-AI cells
-
expressed in Escherichia coli DH5alpha cells
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain M15
-
expression of TbAK in Saccharomyces cerevisiae complements a null mutation in the adenosine kinase gene ado1. The concomitant expression of TbAK with the Trypanosoma brucei adenosine transporter gene TbAT1 allows Saccharomyces cerevisiae ado1 ade2 double mutants to grow on adenosine as the sole purine source and, at the same time, sensitizes them to adenosine antimetabolites
-
expression of viral proteins L2 and AL2 in nucelus and cytosol of transgenic plants via the Agrobacterium infection system highly reduces enzyme activity
-
gene Rv2202c or adoK, phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, overexpression in enzyme-deficient Mycobacterium smegmatis strain SRI101 and in enzyme-deficient Mycobacterium tuberculosis strain SRICKI
overexpressed in Escherichia coli
-
overexpression in Escherichia coli
-
overexpression of the soluble enzyme in Escherichia coli strain BL21(DE3)
-
phylogenetic analysis of the ADK protein family, DNA and amino acid sequence determination and analysis, expression of His-tagged protein in Escherichia coli strain BL21(DE3)