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2.7.1.138: ceramide kinase

This is an abbreviated version!
For detailed information about ceramide kinase, go to the full flat file.

Word Map on EC 2.7.1.138

Reaction

ATP
+
ceramide
=
ADP
 +
ceramide 1-phosphate

Synonyms

acylsphingosine kinase, ceramide kinase, CERK, DCERK protein, hCERK, kinase, acylsphingosine (phosphorylating), More

ECTree

     2 Transferases
         2.7 Transferring phosphorus-containing groups
             2.7.1 Phosphotransferases with an alcohol group as acceptor
                2.7.1.138 ceramide kinase

Cloned

Cloned on EC 2.7.1.138 - ceramide kinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination, expression in HeLa and COS-1 cells, expression of GFP-tagged enzyme in HUVEC and COS-1 cells
-
expressed in CHO cells
expressed in COS-1 cells
expressed in COS-1 fibroblasts and RBL-2H3 basophils
expressed in COS-7 cells
-
expressed in Escherichia coli Top10F cells
expressed in J-774 cells
-
expression in baculovirus-infected SF9 insect cells
-
expression in baculovirus-infected Sf9, overexpression in COS1-CerK cells
-
expression in CHO cells in the plasma membrane
-
expression in Escherichia coli
-
expression in Escherichia coli strain BL21(DE3), expression in CHO cells
expression in Escherichia coli, overexpression of human CerK in COS-1 and RBL-2H3 cells
expression in SF9 insect cell and transfection of FLAG-tagged or GFP-tagged wild type and mutant CerK proteins in COS-1 cell
expression of C-terminally FLAG-tagged and of EGFP-tagged wild-type and mutant enzymes in COS-1 cells
-
expression of green fluorescent protein-tagged human ceramide kinase in CHO-W11A cells, the increase in ceramide 1-phosphate formation by transfection with the vector for human ceramide kinase significantly enhances the Ca2+-ionophore A23187-induced release of arachidonic acid via cytosolic phospholipase A2alpha activation in CHO cells
-
expression of hCERK in COS-1 cells
-
expression of wild-type and mutant enzymes in rat basophilic leukemia RBL-2H3 cells, expression of wild-type and mutant enzymes in CHO cells
-
overexpression in HeLa cells, expression of His6-tagged enzyme in Spodoptera frugiperda Sf9 insect cells using the baculovirus infection system
-
recombinant expression of HA-tagged enzyme CerK in PC-12 cells, A-549cells, and HEK-293 T cells, quantitative real-time PCR analysis of CerK mRNA
recombinant full-length hCERK is obtained from baculovirus-infected Sf9 cells as a glutathione-S-transferase fusion
stable expression in the cytosol and plasma membrane of rat basophilic leukemia RBL-2H3 cells, overexpression of the enzyme enhances the level of degranulation
-
stable expression of human CerK in COS-1 cell, named COS-CerK cell, and RBL-2H3 cell
transient expression in HEK293 cells
transient overexpression of FLAG-tagged wild-type and mutant enzymes in HEK293 cells
-