Cloned (Comment) | Organism |
---|---|
expressed in COS-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K68A/K74A/K80A | shows wild type activity | Homo sapiens |
K90V/K98V | shows partial activity | Homo sapiens |
K90V/R91A | shows partial activity | Homo sapiens |
K90V/R91A/R96A/K98V | totally devoid of activity | Homo sapiens |
R29A/R33A/R36A | shows wild type activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
ATP | assay mixture is composed of 0.88 mM ceramide, 1 mM cardiolipin, 1.5% beta-octylglucoside, 0.2 mM diethylenetriaminepenta-acetic acid, 20 mM MOPS, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 2 mM EGTA, 3 mM CaCl2 and 0.5 mM ATP, at 30°C | Homo sapiens | |
0.052 | - |
ATP | assay mixture is composed of 0.88 mM ceramide, 1 mM cardiolipin, 1.5% beta-octylglucoside, 0.2 mM diethylenetriaminepenta-acetic acid, 20 mM MOPS, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 2 mM EGTA, 3 mM CaCl2 and 0.5 mM ATP, at 30°C | Homo sapiens | |
0.194 | - |
ceramide | assay mixture is composed of 0.88 mM ceramide, 1 mM cardiolipin, 1.5% beta-octylglucoside, 0.2 mM diethylenetriaminepenta-acetic acid, 20 mM MOPS, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 2 mM EGTA, 3 mM CaCl2 and 0.5 mM ATP, at 30°C | Homo sapiens | |
0.205 | - |
ceramide | assay mixture is composed of 0.88 mM ceramide, 1 mM cardiolipin, 1.5% beta-octylglucoside, 0.2 mM diethylenetriaminepenta-acetic acid, 20 mM MOPS, pH 7.2, 50 mM NaCl, 10 mM dithiothreitol, 2 mM EGTA, 3 mM CaCl2 and 0.5 mM ATP, at 30°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Golgi apparatus | - |
Homo sapiens | 5794 | - |
membrane | - |
Homo sapiens | 16020 | - |
nucleus | - |
Homo sapiens | 5634 | - |
vesicle | - |
Homo sapiens | 31982 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8TCT0 | - |
- |
Purification (Comment) | Organism |
---|---|
Sepharose-bead chromatography and Bio-Spin 6 column chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
30°C, 50 mM Tris/HCl, pH 7.5, 270 mM sucrose, 1 mM EDTA, 1 mM EGTA, 5 mM tris(hydroxypropyl)phosphine, 1% Triton X-100, 20 min, 50% decrease of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + ceramide | - |
Homo sapiens | ADP + ceramide 1-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CERK | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |