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2.6.1.42: branched-chain-amino-acid transaminase

This is an abbreviated version!
For detailed information about branched-chain-amino-acid transaminase, go to the full flat file.

Word Map on EC 2.6.1.42

Reaction

L-leucine
+
2-oxoglutarate
=
4-methyl-2-oxopentanoate
 +
L-glutamate

Synonyms

(R)-selective omega-aminotransferase, AGT, alpha-KGA, At1g50110, At5g17310, Bat1, Bat1p, BAT2, Bat2p, BCAA, BCAA aminotransferase, BCAA-AT, BCAATase, BcaT, BCAT-1, BCAT1, Bcat2, BCAT4, Bcat6, BCAT7, BCATc, BCATm, branched chain amino acid: 2-oxoglutarate aminotransferase EC 2.6.1, branched chain aminotransferase, branched chain aminotransferase IlvE, branched chain aminotransferase, cytosolic isoform, branched chain aminotransferase, mitochondrial isoform, branched-chain amino acid aminotransferase, branched-chain amino acid aminotransferase 1, branched-chain amino acid aminotransferase 2, branched-chain amino acid aminotransferase 3, branched-chain amino acid aminotransferase 4, branched-chain amino acid aminotransferase 5, branched-chain amino acid aminotransferase 6, branched-chain amino acid transaminase, branched-chain amino acid transaminase 1, branched-chain amino acid transferase, branched-chain amino acid-glutamate transaminase, branched-chain amino-acid aminotransferase, branched-chain amino-acid transaminase, branched-chain aminotransferase, branched-chain aminotransferase 7, branched-chain aminotransferase4, CsBCAT2, CsBCAT3, CsBCAT7, eBCAT, EC 2.6.1.6, glutamate-branched-chain amino acid transaminase, hBCAT, hBCATm, Hoch3033, IlvE, IlvE aminotransferase, ilvE1, L-branched chain amino acid aminotransferase, L-leucine-alpha-ketoglutarate transaminase, leucine aminotransferase, leucine transaminase, Mevan_0408, mitochondrial branched chain aminotransferase, More, MtIlvE, PsBCAT, R-omegaAT, R-omegaAT_Bcel, R-omegaAT_Bthu, Rv2210c, SlBCAT1, SlBCAT2, SlBCAT3, SlBCAT4, SlBCAT5, SlBCAT6, SpBCAT1, TA-B, transaminase B, trd_1610, Tter_1720, TUZN1299, VMUT0738, VMUT_0738

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.42 branched-chain-amino-acid transaminase

Cloned

Cloned on EC 2.6.1.42 - branched-chain-amino-acid transaminase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a synthetic PsBCAT gene is cloned and heterologously expressed in Escherichia coli strain BL21(DE3) as His-tagged fusion protein
-
BCATm cDNA
cDNA of 6 BCAT genes cloned, transient expression in Nicotiana tabacum protoplasts, complementation analysis in Saccharomyces cerevisiae
-
cloning of ilv gene, Escherichia coli AB2227 transformed
-
cytosolic enzyme, cDNA
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli Rosetta 2 cells
expressed in Escherichia coli strain JM109
-
expressed in Lactobacillus sakei TMW1.1322
expressed in Saccharomyces cerevisiae
expressed in the industrial wine yeast strain Saccharomyces cerevisiae VIN13
-
expressed in transgenic Mus musculus expressing the brain-derived neurotrophic factor cDNA under the control of the alpha-calcium/calmodulin-dependent kinase II promoter
-
expression in Escherichia coli
for complementation experiments
-
gene BAT1, overexpression in Saccharomyces cerevisiae taz1DELTA mutant strain
gene BAT2, overexpression in Saccharomyces cerevisiae taz1DELTA mutant strain
gene BCAT, isozyme BCATm (residues 28-392) deleted of the N-terminal mitochondria signal peptide (residues 1-27) is cloned into kanamycin resistant pET28a with an N-terminal 6-His-thrombin cleavage site and transformed into Escherichia coli strain BL21Star (DE3)
gene BCAT, recombinant expression in a Pichia pastoris strains (DELTAcwp1, DELTAscw10 resulting in deletio of cell wall proteins) OCH1 knockout strain FWK3, the recombinant Escherichia coli enzyme is secreted. Method development and optimization, detailed overview
gene BCAT1, located at 12p12.1, the BCAT1 gene is a target for c-myc
gene BCAT2, located at 19q13.33
gene BCAT2, the BCAT2 protein is fused to an N-terminal His-tag for affinity purification, followed by a tobacco etch virus (TEV) protease cleavage site for tag removal, recombinant expression of His-tagged enzyme
gene ilvE, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene ilvE, recombinant expression of codon-optimized gene encoding the His6-tagged enzyme with TEV proteae cleavage site in Escherichia coli strain BL21(DE3)pLys
gene ilvE, recombinant expression of codon-optimized gene encoding the His6-tagged enzyme with TEV protease cleavage site in Escherichia coli strain BL21(DE3)pLys
gene ilvE1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene Vmut_0738, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His6-tagged enzyme in Escherichia coli strain DLT1270 from vector pQE30_Vmut0738
glutathione S-transferase tagged protein expressed in Escherichia coli
-
glutathione S-transferase tagged protein expressed in Escherichia coli BL21(DE3)
GST-tagged protein expressed in Escherichia coli BL21(DE3)
His-tagged protein expressed in Escherichia coli BL21
His-tagged protein expressed in Escherichia coli BL21(DE3), E-GFP fusion protein expressed in Nicotiana benthamiana
-
His-tagged protein expressed in Escherichia coli BL21(DE3), E-GFP fusion protein expressed in Nicotiana benthamiana, glutathione S-transferase tagged protein expressed in Escherichia coli
-
His-tagged protein expressed in Escherichia coli BL21-DE3
-
His-tagged protein expressed in Escherichia coli BL21AI
His-tagged protein expressed in Escherichia coli JM109
ilvE gene cloned using degenerate oligonucleotides and PCR
-
ilvE gene of Escherichia coli is inserted into the region downstream of the tac-promotor, enzyme overproduced by about a 100fold in Escherichia coli W3110
-
ilvGEDAY gene cluster expressed in Escherichia coli JA199
-
isoform of BCATm, cloned using a yeast two-hybrid system, expressed in transfected monkey kidney CV1 cells
isolation of eca39/BCAT gene
K-12 strain carries the ilvE gene both on the host chromosome and on a plasmid
-
LM0230 ilvE gene identified by complementation in Escherichia coli DL39
-
locus name Csa2G020990, localization on chromosome 2: 2526524-2536725, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression in Arabidosis thaliana ecotype Col-0, quantitative isozyme expression analysis, recombinant expression of GFP-tagged isozyme in Cucumis sativus protoplasts (35S::F1 ATPase N77:GFP is used as a mitochondrial targeting control), recombinant expression of His-tagged isozyme in Escherichia coli strain Rosetta(DE3)
locus name Csa2G352930, localization on chromosome 2: 16313915-16315681, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression in Arabidosis thaliana ecotype Col-0, quantitative isozyme expression analysis, recombinant expression of GFP-tagged isozyme in Cucumis sativus protoplasts (35S::F1 ATPase N77:GFP is used as a mitochondrial targeting control), recombinant expression of His-tagged isozyme in Escherichia coli strain Rosetta(DE3)
locus name Csa6G496970, localization on chromosome 6: 24245649-24253151, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression in Arabidosis thaliana ecotype Col-0, quantitative isozyme expression analysis, recombinant expression of GFP-tagged isozyme in Cucumis sativus protoplasts (35S::F1 ATPase N77:GFP is used as a mitochondrial targeting control), recombinant expression of His-tagged isozyme in Escherichia coli strain Rosetta(DE3)
NCDO 763, gene cloned and sequenced with luciferase gene as the reporter
recombinant BCATm and BCATc
-
recombinant expression of C-terminally GFP-tagged wild-type Bat1 and A234D mutant Bat1 in the Bat1-deletion strain
recombinant expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
RT/PCR of BCATm cDNA
-
vector pET-28a, overexpression of recombinant BCATm and BCATc in Escherichia coli
-