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Literature summary for 2.6.1.42 extracted from

  • Schuster, J.; Knill, T.; Reichelt, M.; Gershenzon, J.; Binder, S.
    Branched-chain aminotransferase4 is part of the chain elongation pathway in the biosynthesis of methionine-derived glucosinolates in Arabidopsis (2006), Plant Cell, 18, 2664-2679.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
4-methylsulfanyl-2-oxobutanoate
-
Arabidopsis thaliana
0.93
-
L-methionine
-
Arabidopsis thaliana
4.86
-
L-leucine
-
Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51900
-
predicted mass Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
S-protein agarose chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
phloem
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-homomethionine + 2-oxoglutarate
-
Arabidopsis thaliana 5-methylthio-2-oxopentanoate + L-glutamate
-
?
L-leucine + 2-oxoglutarate
-
Arabidopsis thaliana 4-methyl-2-oxopentanoate + L-glutamate
-
?
L-methionine + 2-oxoglutarate
-
Arabidopsis thaliana 4-methylsulfanyl-2-oxobutanoate + L-glutamate
-
?
additional information little or no activity is detected with L-isoleucine Arabidopsis thaliana ?
-
?
additional information no activity is observed with 6-methylthio-2-oxohexanoate, 3-methyl-2-oxopentanoate, or 3-methyl-2-oxobutyrate Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
At5g17310
-
Arabidopsis thaliana
BCAT4
-
Arabidopsis thaliana
branched-chain aminotransferase4
-
Arabidopsis thaliana