Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli JM109 | Deinococcus radiodurans |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor diffusion method, PEG 8000, pH 7.4, 20°C | Deinococcus radiodurans |
Protein Variants | Comment | Organism |
---|---|---|
K202R | active site residue, no activity detected | Deinococcus radiodurans |
R145E | no activity detected | Deinococcus radiodurans |
Y71A/R145A | no activity detected | Deinococcus radiodurans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus radiodurans | Q9RTX5 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography (Ni2+) | Deinococcus radiodurans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
62.5% activity with 2-oxoisovalerate, 34.9% activity with 3-methyl-2-oxovalerate, 22.7% activity with 2-oxo-4-methylthiobutyrate, 57.1% activity with 2-oxohexanoate, 51.0% activity with 2-oxovalerate | Deinococcus radiodurans |
180 | - |
substrates 2-oxoisocaproate and L-glutamate, pH 8.3, 25°C | Deinococcus radiodurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-isoleucine + 2-oxoglutarate | - |
Deinococcus radiodurans | 3-methyl-2-oxopentanoic acid + L-glutamate | - |
r | |
L-leucine + 2-oxoglutarate | - |
Deinococcus radiodurans | 4-methyl-2-oxopentanoic acid + L-glutamate | - |
r | |
L-valine + 2-oxoglutarate | - |
Deinococcus radiodurans | 3-methyl-2-oxobutanoic acid + L-glutamate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | crystal structure | Deinococcus radiodurans |
Synonyms | Comment | Organism |
---|---|---|
branched-chain aminotransferase | - |
Deinococcus radiodurans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable at least for 10 min, about 40% activity after 10 min at 65% | Deinococcus radiodurans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Deinococcus radiodurans |