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2.6.1.42: branched-chain-amino-acid transaminase

This is an abbreviated version!
For detailed information about branched-chain-amino-acid transaminase, go to the full flat file.

Word Map on EC 2.6.1.42

Reaction

L-leucine
+
2-oxoglutarate
=
4-methyl-2-oxopentanoate
 +
L-glutamate

Synonyms

(R)-selective omega-aminotransferase, AGT, alpha-KGA, At1g50110, At5g17310, Bat1, Bat1p, BAT2, Bat2p, BCAA, BCAA aminotransferase, BCAA-AT, BCAATase, BcaT, BCAT-1, BCAT1, Bcat2, BCAT4, Bcat6, BCAT7, BCATc, BCATm, branched chain amino acid: 2-oxoglutarate aminotransferase EC 2.6.1, branched chain aminotransferase, branched chain aminotransferase IlvE, branched chain aminotransferase, cytosolic isoform, branched chain aminotransferase, mitochondrial isoform, branched-chain amino acid aminotransferase, branched-chain amino acid aminotransferase 1, branched-chain amino acid aminotransferase 2, branched-chain amino acid aminotransferase 3, branched-chain amino acid aminotransferase 4, branched-chain amino acid aminotransferase 5, branched-chain amino acid aminotransferase 6, branched-chain amino acid transaminase, branched-chain amino acid transaminase 1, branched-chain amino acid transferase, branched-chain amino acid-glutamate transaminase, branched-chain amino-acid aminotransferase, branched-chain amino-acid transaminase, branched-chain aminotransferase, branched-chain aminotransferase 7, branched-chain aminotransferase4, CsBCAT2, CsBCAT3, CsBCAT7, eBCAT, EC 2.6.1.6, glutamate-branched-chain amino acid transaminase, hBCAT, hBCATm, Hoch3033, IlvE, IlvE aminotransferase, ilvE1, L-branched chain amino acid aminotransferase, L-leucine-alpha-ketoglutarate transaminase, leucine aminotransferase, leucine transaminase, Mevan_0408, mitochondrial branched chain aminotransferase, More, MtIlvE, PsBCAT, R-omegaAT, R-omegaAT_Bcel, R-omegaAT_Bthu, Rv2210c, SlBCAT1, SlBCAT2, SlBCAT3, SlBCAT4, SlBCAT5, SlBCAT6, SpBCAT1, TA-B, transaminase B, trd_1610, Tter_1720, TUZN1299, VMUT0738, VMUT_0738

ECTree

     2 Transferases
         2.6 Transferring nitrogenous groups
             2.6.1 Transaminases
                2.6.1.42 branched-chain-amino-acid transaminase

Storage Stability

Storage Stability on EC 2.6.1.42 - branched-chain-amino-acid transaminase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, concentrated solution frozen for 1month retains 90% of her original activity
-
-20°C, 0.05 M potassium phosphate buffer, pH 7.5, 10% glycerol, 1 mN DTT, 0.1 M pyridoxal 5'-phosphate, purified enzyme can be stored at least for 3 months without loss of activity
-
-20°C, storage results in a very rapid loss of all activity
-25°C, partially purified enzyme is stable for several weeks
-
-70°C, when stored in presence of dithiothreitol activity is stable for at least 4 weeks
-
0°C, storage results in a very rapid loss of all activity
2°C, preparation concentrated by ultracentrifugation, enzyme retains 75% of its activity for 4 months
-
4°C,stable for several weeks without addition of dithiothreitol
-
5°C, pH 7.0, purified enzyme loses activity, about 20% in 1 week
-
5°C, remarkably stable in crude extract when stored in 0.05 M sodium caproate, 0.5 mM EDTA, pH 6.1, no loss of activity after 1 month
-
5°C, stored in 0.05 M sodium diphosphate, 0.1 M 2-mercaptoethanol, pH 8.5, loses about 50% activity over a period of several days
-
6°C, fairly stable for 1 week