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0.000087
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate
0.00022
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and pyruvate
0.00027
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and 2-oxoglutarate
0.00042
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates (R)-alpha-methylbenzylamine and pyruvate
0.0014
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates L-leucine and 2-oxoglutarate
0.0015
purified recombinant enzyme, pH 7.0, temperature not specified in the publication, substrates L-leucine and 2-oxoglutarate
0.0042
purified enzyme, pH 10.0, 40°C, substrate 2-oxoglutarate
0.0076
substrate 2-methylbenzylamine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
0.025
purified enzyme, pH 10.0, 40°C, substrate 2-oxobutyrate
0.033
purified recombinant enzyme, pH 8.0, 50°C, substrates (R)-1-phenylethylamine and pyruvate
0.035
purified recombinant enzyme, pH 9.0, 50°C, substrates (R)-1-phenylethylamine and 2-oxoglutarate
0.036
-
SlBCAT6, substrate L-leucine, pH not specified in the publication, 25°C
0.054
-
SlBCAT5, substrate 3-methyl-2-oxobutanoic acid, pH not specified in the publication, 25°C
0.072
-
SlBCAT6, substrate 4-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.084
-
SlBCAT4, substrate 4-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.132
-
SlBCAT5, substrate 4-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.135
purified enzyme, pH 10.0, 40°C, substrate 2-oxohexanoate
0.14
-
SpBCAT1, substrate L-valine, pH not specified in the publication, 25°C
0.19
purified enzyme, pH 10.0, 40°C, substrate 3-methyl-2-oxovalerate
0.264
-
SlBCAT3, substrate L-valine, pH not specified in the publication, 25°C
0.276
-
SlBCAT6, substrate 3-methyl-2-oxobutanoic acid, pH not specified in the publication, 25°C
0.282
-
SlBCAT5, substrate L-leucine, pH not specified in the publication, 25°C
0.288
-
SlBCAT3, substrate L-leucine, pH not specified in the publication, 25°C
0.294
-
SlBCAT5, substrate L-valine, pH not specified in the publication, 25°C
0.39
-
SlBCAT5, substrate L-isoleucine, pH not specified in the publication, 25°C
0.41
-
4-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.414
-
SlBCAT3, substrate L-isoleucine, pH not specified in the publication, 25°C
0.7
-
substrate L-phenylalanine
0.7273
-
brain enzyme, substrate L-leucine
0.77
substrate L-alanine, pH 8.0, 65°C
0.83
substrate 3-methyl-2-oxovalerate, purified recombinant His-tagged enzyme, pH 8.0, 65°C
1
substrate L-isoleucine, pH 8.0, 65°C
1.1
substrate DL-tryptophan, purified recombinant His-tagged enzyme, pH 8.0, 65°C
1.5
pH 7.5, 37°C, substrate L-leucine
1.72
substrate L-leucine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
1.74
glycine, pH 8.0, 3°C
1.82
-
heart muscle enzyme, substrate L-leucine
1.88
substrate indole-3-pyruvate, purified recombinant His-tagged enzyme, pH 8.0, 65°C
104.6
L-isoleucine, pH 8.0, 3°C
11.82
-
substrate L-valine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C
125
-
recombinant mitochondrial enzyme
13.44
-
substrate L-leucine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C
14.35
-
substrate L-isoleucine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C
15.9
-
substrate L-valine + 2-oxoglutarate
18.6
-
mitochondria of AS-30D cell, pH 7.8, 37°C
180
substrates 2-oxoisocaproate and L-glutamate, pH 8.3, 25°C
2.17
-
substrate L-phenylalanine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C
2.4
-
substrate L-methionine
2.58
-
substrate L-valine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C
2.64
substrate L-arginine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
2.96
substrate L-norvaline, purified recombinant His-tagged enzyme, pH 8.0, 65°C
2.97
substrate 2-aminobutyrate, purified recombinant His-tagged enzyme, pH 8.0, 65°C
25.3
-
in presence of 2-mercaptoethanol
3.1
substrate 2-oxobutyrate, purified recombinant His-tagged enzyme, pH 8.0, 65°C
3.56
substrate L-threonine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
3.6
substrate L-valine, pH 8.0, 65°C
3.65
-
substrate L-leucine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C
3.9
substrate L-ornithine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
3.93
substrate DL-lysine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
37
purified recombinant enzyme, pH 8.0, 40°C, substrates L-leucine and 2-oxoglutarate
390
-
pH 7.3, 90°C, substrate L-leucine
4.22
-
substrate 4-methylthio-2-oxo-butanoate, cosubstrate leucine, pH 7.4, 37°C
4.28
-
substrate L-isoleucine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C
4.37
substrate L-methionine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
41.8
pH 8.0, 37°C, substrate L-leucine
42
purified recombinant enzyme, pH 8.0, 40°C, substrates L-leucine and 2-oxoglutarate
47
-
substrate L-isoleucine
48.8
-
mitochondrial enzyme
5.23
L-tryptophan, pH 8.0, 3°C
5.7
-
substrate L-glutamate, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C
7
-
regenerating liver, pH 7.8, 37°C
77.03
L-valine, pH 8.0, 3°C
85
-
recombinant cytosolic enzyme
86.84
L-leucine, pH 8.0, 3°C
90
pH 8.0, 37°C, substrate L-leucine
0.007
purified enzyme, pH 10.0, 40°C, substrate pyruvate
0.007
purified recombinant enzyme, pH 8.0, 50°C, substrates (R)-1-phenylethylamine and pyruvate
0.012
-
SlBCAT2, substrate L-valine, pH not specified in the publication, 25°C
0.012
purified recombinant enzyme, pH 8.0, 50°C, substrates (R)-1-phenylethylamine and pyruvate
0.012
purified recombinant enzyme, pH 9.0, 50°C, substrates (R)-1-phenylethylamine and 2-oxoglutarate
0.018
-
L-isoleucine, pH not specified in the publication, 25°C
0.018
-
L-leucine, pH not specified in the publication, 25°C
0.018
-
SlBCAT2, substrate L-isoleucine, pH not specified in the publication, 25°C
0.018
-
SlBCAT2, substrate L-leucine, pH not specified in the publication, 25°C
0.03
-
SlBCAT1, substrate 4-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.03
-
3-methyl-2-oxobutanoic acid, pH not specified in the publication, 25°C
0.042
-
SlBCAT1, substrate 3-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.042
-
SlBCAT4, substrate L-leucine, pH not specified in the publication, 25°C
0.042
-
4-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.048
-
SlBCAT4, substrate 3-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.048
-
SlBCAT4, substrate L-isoleucine, pH not specified in the publication, 25°C
0.048
-
SlBCAT4, substrate L-valine, pH not specified in the publication, 25°C
0.048
-
SlBCAT6, substrate L-isoleucine, pH not specified in the publication, 25°C
0.06
-
SlBCAT1, substrate 3-methyl-2-oxobutanoic acid, pH not specified in the publication, 25°C
0.06
-
SlBCAT3, substrate 3-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.06
-
SlBCAT5, substrate 3-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.06
-
SlBCAT6, substrate 3-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.06
-
SlBCAT6, substrate L-valine, pH not specified in the publication, 25°C
0.066
-
3-methyl-2-oxobutanoic acid, pH not specified in the publication, 25°C
0.066
-
L-valine, pH not specified in the publication, 25°C
0.066
-
SlBCAT3, substrate 4-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.096
-
SlBCAT1, substrate L-isoleucine, pH not specified in the publication, 25°C
0.096
-
SlBCAT1, substrate L-leucine, pH not specified in the publication, 25°C
0.096
-
L-leucine, pH not specified in the publication, 25°C
0.096
-
L-valine, pH not specified in the publication, 25°C
0.114
-
SlBCAT3, substrate 3-methyl-2-oxobutanoic acid, pH not specified in the publication, 25°C
0.114
-
L-isoleucine, pH not specified in the publication, 25°C
0.12
purified enzyme, pH 10.0, 40°C, substrate 3-methyl-2-oxobutyrate
0.12
-
SlBCAT1, substrate L-valine, pH not specified in the publication, 25°C
0.15
purified enzyme, pH 10.0, 40°C, substrate 4-methyl-2-oxovalerate
0.15
-
SlBCAT4, substrate 3-methyl-2-oxobutanoic acid, pH not specified in the publication, 25°C
0.15
purified recombinant enzyme, pH 9.0, 50°C, substrates (R)-1-phenylethylamine and 2-oxoglutarate
0.17
-
SlBCAT2, substrate 3-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.17
-
SpBCAT1, substrate L-isoleucine, pH not specified in the publication, 25°C
0.2
-
3-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.2
-
SpBCAT1, substrate L-leucine, pH not specified in the publication, 25°C
0.21
-
SlBCAT2, substrate 3-methyl-2-oxobutanoic acid, pH not specified in the publication, 25°C
0.21
-
SlBCAT2, substrate 4-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.21
-
3-methyl-2-oxopentanoic acid, pH not specified in the publication, 25°C
0.23
purified enzyme, pH 10.0, 40°C, substrate 2-oxovalerate
0.23
substrate pyruvate, with valine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
1.6
substrate L-cysteine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
1.6
substrate L-phenylalanine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
1.65
substrate 4-methyl-2-oxovalerate, purified recombinant His-tagged enzyme, pH 8.0, 65°C
1.65
substrate L-norleucine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
1.7
substrate L-leucine, pH 8.0, 65°C
1.7
pH 8.0, 65°C, substrate L-leucine
1.7
pH 8.0, 65°C, substrate L-leucine
1.76
substrate L-histidine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
1.76
substrate L-isoleucine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
11.1
-
pH 7.4, 37°C
11.1
pH 9.0, 37°C, substrate L-isoleucine
12.8
-
substrate 2-oxoglutarate, cosubstrate L-leucine, pH 7.4, 37°C
12.8
pH 7.4, 37°C, substrate L-leucine
2.5
-
-
2.5
-
pH 8.0, 30°C, substrate L-leucine
23.9
-
-
23.9
pH 8.0, 25°C, substrate L-leucine
25
-
substrate L-valine
27.3
-
substrate L-isoleucine + 2-oxoglutarate
27.3
pH 8.0, 37°C, substrate L-isoleucine
3
purified recombinant enzyme, pH 8.0, 50°C, substrates L-leucine and 2-oxoglutarate
3
substrate L-valine, purified recombinant His-tagged enzyme, pH 8.0, 65°C
42.8
-
-
42.8
pH 9.0, 37°C, substrate L-leucine
94
substrate L-isoleucine + 2-oxoglutarate
94
-
pH 9.0, 37°C, substrate L-isoleucine
additional information
62.5% activity with 2-oxoisovalerate, 34.9% activity with 3-methyl-2-oxovalerate, 22.7% activity with 2-oxo-4-methylthiobutyrate, 57.1% activity with 2-oxohexanoate, 51.0% activity with 2-oxovalerate
additional information
-
62.5% activity with 2-oxoisovalerate, 34.9% activity with 3-methyl-2-oxovalerate, 22.7% activity with 2-oxo-4-methylthiobutyrate, 57.1% activity with 2-oxohexanoate, 51.0% activity with 2-oxovalerate
additional information
-
final preparation, specific activity 23.9 U/mg
additional information
-
specific activity 4660 units, 1 unit is defined as the change of absorbance units at 348 nm and 37° within 30 min