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K202R
active site residue, no activity detected
R145E
no activity detected
Y71A/R145A
no activity detected
C221S
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the mutant shows strongly decreased kcat values compared to the wild type enzyme
C235S
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the mutant enzyme exhibits kcat values that are within 20-30% of wild type BCATc values, but also have lower Km values, hence higher calculated kcat/Km values than those of the wild type enzyme
C242S
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the mutant shows strongly decreased kcat values compared to the wild type enzyme
C293S
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the C293S mutant and wild type BCATc have similar kinetic constants with L-valine, L-leucine, and L-glutamate
C315A/C318A
contains changes in the structure of the beta-turn preceding the CXXC motif when compared with wild type protein, the oxidized mutant enzyme shows limited activity
C318A/C315CSD
the overall structure of the C318A/C315CSD variant overlays very well with that of the C318A mutant and the oxidized form of wild-type hBCATm, with a few exceptions in the interdomain loop (residues 171-181) and the N-terminal loop (residues 15-32)
C335S
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for all amino acid substrates, there is a significant decrease (70% to 80%) in kcat with the C335S mutant, the Km value for glutamate with the C335S mutant enzyme is approximately 3fold lower than that observed with wild type enzyme
C335S/C3388S
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the mutant shows kcat values that are similar to values observed with the single C335 mutant enzymes, Km values are largely unchanged compared to those of wild type BCATc, kcat/Km values are significantly lower than wild type values
C335S/C338S
dimerization of BCATc occurs concurrently with GSNO-mediated S-glutathionylation, which is abolished when substituted with the double mutant C335S/C338S
C338S
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the mutant enzyme exhibits kcat values for the BCAAs that were 50% lower than that observed with wild type enzyme. With the exception of L-glutamate, where the Km value is not changed and L-valine, where the Km value is 50% lower than the value for wild type BCATc, Km values for L-isoleucine and L-leucine are largely unaffected for the C338S mutant
E264K
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heterogeneous BCAT2 gene mutation, in combination heterogeneous BCAT2 gene mutation R170Q, found in a 25-year-old patient presenting with headache complaints and mild memory impairment for about six years
R170Q
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heterogeneous BCAT2 gene mutation, in combination heterogeneous BCAT2 gene mutation E264K, found in a 25-year-old patient presenting with headache complaints and mild memory impairment for about six years
T186R
site-directed mutagenesis, structure determination and comparison with wild-type enzyme
A234D
site-directed mutagenesis of strain BY4741, both deletion mutant bat1DELTA and point mutant bat1A234D cells exhibit the same phenotypes relative to the wild-type Bat1 strain, namely, a repressive growth rate in the logarithmic phase, decreases in intracellular valine and leucine content in the logarithmic and stationary growth phases, respectively, and an increase in fusel alcohol content in culture medium, and a decrease in the carbon dioxide productivity. These results indicate that amino acid change from Ala to Asp at position 234 leads to a functional impairment of Bat1, although homology modeling suggests that Asp234 in the variant Bat1 does not inhibit enzymatic activity directly
K202A
site-directed mutagenesis, inactive mutant
K219R
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mutation in conserved pyridoxal phosphate-binding site, loss of activity. Mutant is nearly as effective aswild-type Bat1 or Bat2 at partially suppressing the rapamycin recovery and TORC1 activity defects of the Bat1 bat2 mutant
A234D
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site-directed mutagenesis of strain BY4741, both deletion mutant bat1DELTA and point mutant bat1A234D cells exhibit the same phenotypes relative to the wild-type Bat1 strain, namely, a repressive growth rate in the logarithmic phase, decreases in intracellular valine and leucine content in the logarithmic and stationary growth phases, respectively, and an increase in fusel alcohol content in culture medium, and a decrease in the carbon dioxide productivity. These results indicate that amino acid change from Ala to Asp at position 234 leads to a functional impairment of Bat1, although homology modeling suggests that Asp234 in the variant Bat1 does not inhibit enzymatic activity directly
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H106Y/Y108R
site-directed mutagenesis
H106Y/Y108R
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site-directed mutagenesis
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H106Y/Y108R
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site-directed mutagenesis
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H106Y/Y108R
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site-directed mutagenesis
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C315A
contains changes in the structure of the beta-turn preceding the CXXC motif when compared with wild type protein, the oxidized mutant enzyme shows limited activity
C315A
analysis of the PLP-bound mutant enzyme crystal structure, ODB ID 2HG8
C315A
site-directed mutagenesis, mutation of the redox sensor (Cys315) results in a significant loss of activity compared to wild-type
C318A
contains changes in the structure of the beta-turn preceding the CXXC motif when compared with wild type protein, the oxidized mutant enzyme shows limited activity
C318A
site-directed mutagenesis, the mutant shows unaltered activity compared to wild-type, structure analysis
K219A
site-directed mutagenesis, inactive mutant
K219A
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mutation in conserved pyridoxal phosphate-binding site, loss of activity. Mutant is nearly as effective aswild-type Bat1 or Bat2 at partially suppressing the rapamycin recovery and TORC1 activity defects of the Bat1 bat2 mutant
additional information
phenotypic analysis of 35S::CsBCAT2 Arabidopsis thaliana transgenic plants, overview. CsBCATs regulate the expression levels of FT/SOC1 via control of GI/CO and the SVP/FLC expression, respectively, for the modulation of flowering in Arabidopsis thaliana
additional information
phenotypic analysis of 35S::CsBCAT2 Arabidopsis thaliana transgenic plants, overview. CsBCATs regulate the expression levels of FT/SOC1 via control of GI/CO and the SVP/FLC expression, respectively, for the modulation of flowering in Arabidopsis thaliana
additional information
phenotypic analysis of 35S::CsBCAT2 Arabidopsis thaliana transgenic plants, overview. CsBCATs regulate the expression levels of FT/SOC1 via control of GI/CO and the SVP/FLC expression, respectively, for the modulation of flowering in Arabidopsis thaliana
additional information
phenotypic analysis of 35S::CsBCAT3 Arabidopsis thaliana transgenic plants, overview. CsBCATs regulate the expression levels of FT/SOC1 via control of GI/CO and the SVP/FLC expression, respectively, for the modulation of flowering in Arabidopsis thaliana
additional information
phenotypic analysis of 35S::CsBCAT3 Arabidopsis thaliana transgenic plants, overview. CsBCATs regulate the expression levels of FT/SOC1 via control of GI/CO and the SVP/FLC expression, respectively, for the modulation of flowering in Arabidopsis thaliana
additional information
phenotypic analysis of 35S::CsBCAT3 Arabidopsis thaliana transgenic plants, overview. CsBCATs regulate the expression levels of FT/SOC1 via control of GI/CO and the SVP/FLC expression, respectively, for the modulation of flowering in Arabidopsis thaliana
additional information
phenotypic analysis of 35S::CsBCAT7 Arabidopsis thaliana transgenic plants, overview. CsBCATs regulate the expression levels of FT/SOC1 via control of GI/CO and the SVP/FLC expression, respectively, for the modulation of flowering in Arabidopsis thaliana
additional information
phenotypic analysis of 35S::CsBCAT7 Arabidopsis thaliana transgenic plants, overview. CsBCATs regulate the expression levels of FT/SOC1 via control of GI/CO and the SVP/FLC expression, respectively, for the modulation of flowering in Arabidopsis thaliana
additional information
phenotypic analysis of 35S::CsBCAT7 Arabidopsis thaliana transgenic plants, overview. CsBCATs regulate the expression levels of FT/SOC1 via control of GI/CO and the SVP/FLC expression, respectively, for the modulation of flowering in Arabidopsis thaliana
additional information
Pichia pastoris strain CBS7435 MutS is used as parent strain for BCAT expressions. The improved BCAT secretion by OCH1 knockout in Pichia pastoris, provides a good system for efficient chiral amine production. Method improvement leads to increase of the extracellular BCAT production, but also to achieve a more homogenous product in terms of glycosylation, identification of a deletion strain that counteracts typical cell clustering in the Pichia pastoris DELTAoch1 strain. The knockout of the locus OCH1, strain FWK3, leads to a more homogenous glycosylation: more Man8 than Man10 N-glycans are added during the mannosylation process, facilitating for example the handling for downstream processes, since hypermannosylation and non homogenous glycosylation can be largely avoided
additional information
isozyme BCAT1 knockdown represses the growth rate of MCF-7 and T47D cells since day 2. mitochondrial BCAT1 knockdown reduces mitochondrial DNA content, whereas BCAT1 overexpression increases mitochondrial DNA content in MCF-7 and T47D cells. BCAT1 knockdown represses mTOR signaling, and BCAT1 overexpression activates mTOR signaling
additional information
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isozyme BCAT1 knockdown represses the growth rate of MCF-7 and T47D cells since day 2. mitochondrial BCAT1 knockdown reduces mitochondrial DNA content, whereas BCAT1 overexpression increases mitochondrial DNA content in MCF-7 and T47D cells. BCAT1 knockdown represses mTOR signaling, and BCAT1 overexpression activates mTOR signaling
additional information
construction of two artificial genes encoding the mitochondrial-targeting signal (MTS)-deleted Bat1 (Bat1-MTS) and the MTS of Bat1-fused Bat2 (Bat2+MTS) from originating Bat1 and Bat2 genes. Bat2+MTS is relocalized into the mitochondria, because Bat2 localization is changed from cytosol to the mitochondria by addition of MTS, and can partially restore the valine content and growth in DELTAbat1DELTAbat2 cells. Bat1-MTS and Bat2+MTS function properly in DELTAbat1DELTAbat2 cells. Mutant DELTAbat1DELTAbat2 cells harboring Bat1-MTS grow similarly to DELTAbat1 cells
additional information
construction of two artificial genes encoding the mitochondrial-targeting signal (MTS)-deleted Bat1 (Bat1-MTS) and the MTS of Bat1-fused Bat2 (Bat2+MTS) from originating Bat1 and Bat2 genes. Bat2+MTS is relocalized into the mitochondria, because Bat2 localization is changed from cytosol to the mitochondria by addition of MTS, and can partially restore the valine content and growth in DELTAbat1DELTAbat2 cells. Bat1-MTS and Bat2+MTS function properly in DELTAbat1DELTAbat2 cells. Mutant DELTAbat1DELTAbat2 cells harboring Bat1-MTS grow similarly to DELTAbat1 cells
additional information
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construction of two artificial genes encoding the mitochondrial-targeting signal (MTS)-deleted Bat1 (Bat1-MTS) and the MTS of Bat1-fused Bat2 (Bat2+MTS) from originating Bat1 and Bat2 genes. Bat2+MTS is relocalized into the mitochondria, because Bat2 localization is changed from cytosol to the mitochondria by addition of MTS, and can partially restore the valine content and growth in DELTAbat1DELTAbat2 cells. Bat1-MTS and Bat2+MTS function properly in DELTAbat1DELTAbat2 cells. Mutant DELTAbat1DELTAbat2 cells harboring Bat1-MTS grow similarly to DELTAbat1 cells
additional information
generation of a BY4741 bat1 deletion strain, phenotype, overview
additional information
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generation of a BY4741 bat1 deletion strain, phenotype, overview
additional information
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generation of a BY4741 bat1 deletion strain, phenotype, overview
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additional information
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mutant deficient in enzymic activity, requires 2-methylbutanoate or 2-methylpropanoate for growth in defined medium. Mutant shows extensive alterations in the fatty acid composition in the cell membrane. Mutant is still able to produce 3-methlybutanoate and 2-methylpropanoate when cultivated in rich medium