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Literature summary for 2.6.1.42 extracted from
- Purification of branched chain aminotransferase from rat heart mitochondria (1990), J. Biol. Chem., 265, 6019-6024.
General Stability
| General Stability | Organism |
|---|---|
| not stable to freezing without addition of dithiothreitol | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
1 * 43000, SDS-PAGE | Rattus norvegicus |
| 50000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-isoleucine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r |  |
| L-isoleucine + 2-oxoglutarate | Rattus norvegicus Sprague-Dawley | - |
3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
| L-leucine + 2-oxoglutarate | Rattus norvegicus | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
| L-leucine + 2-oxoglutarate | Rattus norvegicus Sprague-Dawley | - |
4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
| L-valine + 2-oxoglutarate | Rattus norvegicus | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
| L-valine + 2-oxoglutarate | Rattus norvegicus Sprague-Dawley | - |
3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
Sprague-Dawley | - |
| Rattus norvegicus Sprague-Dawley | - |
Sprague-Dawley | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| heart | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 66 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, when stored in presence of dithiothreitol activity is stable for at least 4 weeks | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-isoleucine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
| L-isoleucine + 2-oxoglutarate | - |
Rattus norvegicus Sprague-Dawley | 3-methyl-2-oxopentanoate + L-glutamate | - |
r | |
| L-leucine + 2-oxoglutarate | - |
Rattus norvegicus | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
| L-leucine + 2-oxoglutarate | - |
Rattus norvegicus Sprague-Dawley | 4-methyl-2-oxopentanoate + L-glutamate | - |
r | |
| L-valine + 2-oxoglutarate | - |
Rattus norvegicus | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
| L-valine + 2-oxoglutarate | - |
Rattus norvegicus Sprague-Dawley | 3-methyl-2-oxobutanoate + L-glutamate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Rattus norvegicus |
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