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0.65
(3S)-2-deoxyerythrose 4-phosphate
-
pH 6.8
0.006
2-deoxy-D-erythrose 4-phosphate
pH 6.8, 60°C
2.5
2-deoxy-D-ribose 5-phosphate
pH 6.8, 60°C
6.8
2-deoxyribose 5-phosphate
-
recombinant enzyme, pH 7.5, 25°C
0.03 - 2.7
D-arabinose 5-phosphate
2.5
D-erythrose
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
0.0026 - 52.36
D-erythrose 4-phosphate
3.5
D-glyceraldehyde
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
1.58
D-ribose 5-phosphate
pH 6.8, 60°C
8.4
D-threose
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
3
DL-glyceraldehyde 3-phosphate
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
8.6
glycolaldehyde
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
3.6
glyoxylate
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
5.1
L-erythrose
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
3.3
L-glyceraldehyde
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
13.9
L-threose
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
0.003 - 21
phosphoenolpyruvate
6
ribose 5-phosphate
-
recombinant enzyme, pH 7.5, 25°C
additional information
additional information
-
0.03
D-arabinose 5-phosphate
-
recombinant enzyme, pH 7.5, 25°C
2.7
D-arabinose 5-phosphate
pH 6.8, 60°C
0.0026
D-erythrose 4-phosphate
-
pH 7.0, 37°C
0.006
D-erythrose 4-phosphate
-
pH 7.5, 30°C
0.009
D-erythrose 4-phosphate
pH 6.8, 60°C
0.013
D-erythrose 4-phosphate
-
pH 7.4, 37°C
0.013
D-erythrose 4-phosphate
pH 7.3, temperature not specified in the publication, wild-type enzyme
0.0134
D-erythrose 4-phosphate
pH 6.8, 25°C, mutant R126S
0.015
D-erythrose 4-phosphate
pH 7.5, 25°C
0.016
D-erythrose 4-phosphate
pH 7.5, 37°C, presence of Co2+
0.018
D-erythrose 4-phosphate
-
wild-type, pH 7.9, 30°C
0.021
D-erythrose 4-phosphate
-
pH 6.8
0.028
D-erythrose 4-phosphate
-
60°C, pH 6.8
0.028
D-erythrose 4-phosphate
pH 7.3, temperature not specified in the publication, mutant H29S/S31H
0.03
D-erythrose 4-phosphate
pH 7.3, temperature not specified in the publication, mutant H29A
0.033
D-erythrose 4-phosphate
pH 7.3, temperature not specified in the publication, mutant S31G
0.037
D-erythrose 4-phosphate
pH 6.8, 25°C, wild-type enzyme
0.04
D-erythrose 4-phosphate
pH 6.8, 30°C, mutant R126S
0.043
D-erythrose 4-phosphate
pH 6.8, 30°C, wild-type enzyme
0.055
D-erythrose 4-phosphate
-
pH 7.8, 25°C
0.0573
D-erythrose 4-phosphate
-
pH 7.5, 50°C
0.07
D-erythrose 4-phosphate
-
pH 7.0, 25°C
0.071
D-erythrose 4-phosphate
pH 7.5, 60°C, mutant enzyme I181D
0.0814
D-erythrose 4-phosphate
-
Tyr-sensitive isozyme, 25°C
0.086
D-erythrose 4-phosphate
-
Phe-sensitive isozyme, pH 6.8, 25°C
0.092
D-erythrose 4-phosphate
pH 7.5, 60°C, wild-type enzyme
0.0965
D-erythrose 4-phosphate
-
pH 7.0, 37°C
0.13
D-erythrose 4-phosphate
-
Phe-sensitive isozyme, pH 6.8, 30°C
0.138
D-erythrose 4-phosphate
-
pH 7.5, 60°C
0.141
D-erythrose 4-phosphate
-
recombinant enzyme, pH 7.5, 25°C
0.16
D-erythrose 4-phosphate
-
30°C, pH 7.0
0.195
D-erythrose 4-phosphate
-
Trp-sensitive isozyme, pH 7.0, 30°C
0.25
D-erythrose 4-phosphate
-
Trp-sensitive isozyme, pH 7.2, 37°C
0.25
D-erythrose 4-phosphate
-
wild-type, 25°C, pH 6.4
0.278
D-erythrose 4-phosphate
-
mutant I10A, 25°C, pH 6.4
0.28
D-erythrose 4-phosphate
pH 7.0, 60°C
0.29
D-erythrose 4-phosphate
-
isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C
0.3506
D-erythrose 4-phosphate
-
pH 7.5, 70°C
0.392
D-erythrose 4-phosphate
-
mutant N5K, 25°C, pH 6.4
0.43
D-erythrose 4-phosphate
pH 7.5, 30°C
0.5
D-erythrose 4-phosphate
-
Tyr-sensitive isozyme, pH 6.8
0.56
D-erythrose 4-phosphate
-
DAHP synthase-trp, pH 7.0, 37°C
0.77
D-erythrose 4-phosphate
-
DAHP synthase-tyr, pH 7.0, 37°C
0.87
D-erythrose 4-phosphate
-
DAHP synthase-phe, pH 7.0, 37°C
0.9
D-erythrose 4-phosphate
-
pH 7.0, 37°C
1
D-erythrose 4-phosphate
-
DAHP synthase-tyr, pH 7.0, 37°C
1.1
D-erythrose 4-phosphate
-
DS II isozyme, pH 7.5, 37°C
1.2
D-erythrose 4-phosphate
-
pH 6.4, 37°C
1.4
D-erythrose 4-phosphate
-
at 37°C
1.76
D-erythrose 4-phosphate
-
pH 7.5, 37°C
1.95
D-erythrose 4-phosphate
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
2.17
D-erythrose 4-phosphate
-
isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5,at 30°C
3.3
D-erythrose 4-phosphate
-
cytosolic isozyme, pH 8.6
3.638
D-erythrose 4-phosphate
-
at 37°C
52.36
D-erythrose 4-phosphate
-
N-terminal deletion mutant, 25°C, pH 6.4
0.003
phosphoenolpyruvate
-
pH 7.5, 30°C
0.0032
phosphoenolpyruvate
-
wild-type, pH 7.9, 30°C
0.0043
phosphoenolpyruvate
-
recombinant wild-type, 30°C, pH 6.5
0.00485
phosphoenolpyruvate
pH 7.3, temperature not specified in the publication, wild-type enzyme
0.005
phosphoenolpyruvate
-
recombinant enzyme, with D-arabinose 5-phosphate as cosubstrate, pH 7.5, 25°C
0.0053
phosphoenolpyruvate
-
recombinant enzyme, with erythrose 4-phosphate as cosubstrate, pH 7.5, 25°C
0.0058
phosphoenolpyruvate
-
pH 7.0, 37°C
0.0067
phosphoenolpyruvate
-
pH 7.0, 37°C
0.0087
phosphoenolpyruvate
pH 6.8, 30°C, mutant S213G
0.009
phosphoenolpyruvate
-
pH 7.5
0.009
phosphoenolpyruvate
-
Phe-sensitive isozyme, pH 6.8, 25°C
0.0095
phosphoenolpyruvate
-
pH 7.5, 70°C
0.01
phosphoenolpyruvate
-
recombinant enzyme, with ribose 5-phosphate as cosubstrate, pH 7.5, 25°C
0.011
phosphoenolpyruvate
pH 6.8, 30°C, wild-type enzyme
0.0117
phosphoenolpyruvate
-
pH 7.5, 60°C
0.0128
phosphoenolpyruvate
pH 7.3, temperature not specified in the publication, mutant S31G
0.013
phosphoenolpyruvate
-
pH 7.5, 50°C
0.013
phosphoenolpyruvate
-
Tyr-sensitive isozyme, 25°C
0.015
phosphoenolpyruvate
-
pH 7.4, 37°C
0.015
phosphoenolpyruvate
-
recombinant enzyme, with 2-deoxyribose 5-phosphate as cosubstrate, pH 7.5, 25°C
0.015
phosphoenolpyruvate
pH 6.8, 25°C, wild-type enzyme
0.017
phosphoenolpyruvate
pH 7.5, 37°C, presence of Co2+
0.018
phosphoenolpyruvate
-
Phe-sensitive isozyme, pH 6.8, 30°C
0.0196
phosphoenolpyruvate
pH 8.0, 40°C
0.021
phosphoenolpyruvate
pH 7.3, temperature not specified in the publication, mutant H29A
0.025
phosphoenolpyruvate
pH 6.8, 25°C, mutant R126S
0.028
phosphoenolpyruvate
-
mutant N5K, 25°C, pH 6.4
0.03
phosphoenolpyruvate
-
pH 7.0, 25°C
0.031
phosphoenolpyruvate
-
mutant I10A, 25°C, pH 6.4
0.033
phosphoenolpyruvate
pH 6.8, 60°C, cosubstrate 2-deoxy-D-erythrose 4-phosphate
0.035
phosphoenolpyruvate
pH 6.8, 60°C, cosubstrate 2-deoxy-D-ribose 5-phosphate
0.035
phosphoenolpyruvate
-
wild-type, 25°C, pH 6.4
0.036
phosphoenolpyruvate
pH 6.8, 60°C, cosubstrate D-ribose 5-phosphate
0.039
phosphoenolpyruvate
pH 7.3, temperature not specified in the publication, mutant H29S/S31H
0.043
phosphoenolpyruvate
-
pH 7.8, 25°C
0.062
phosphoenolpyruvate
pH 6.8, 60°C, cosubstrate D-arabinose 5-phosphate
0.066
phosphoenolpyruvate
pH 7.5, 60°C, mutant enzyme I181D
0.08
phosphoenolpyruvate
-
37°C, pH 7.0
0.09
phosphoenolpyruvate
pH 7.5, 30°C
0.092
phosphoenolpyruvate
-
Trp-sensitive isozyme, pH 7.0, 30°C
0.093
phosphoenolpyruvate
pH 6.8, 60°C, cosubstrate D-erythrose 4-phosphate
0.112
phosphoenolpyruvate
pH 7.5, 60°C, wild-type enzyme
0.12
phosphoenolpyruvate
-
60°C, pH 6.8
0.125
phosphoenolpyruvate
-
Tyr-sensitive isozyme, pH 6.8
0.139
phosphoenolpyruvate
-
pH 7.5, 37°C
0.14
phosphoenolpyruvate
pH 7.5, 25°C
0.1415
phosphoenolpyruvate
-
at 37°C
0.16
phosphoenolpyruvate
-
isozyme NCgl0950 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C
0.2
phosphoenolpyruvate
-
DAHP synthase-phe, pH 7.0, 37°C
0.26
phosphoenolpyruvate
-
DAHP synthase-tyr, pH 7.0, 37°C
0.3
phosphoenolpyruvate
-
30°C, pH 7.0
0.4
phosphoenolpyruvate
-
Trp-sensitive isozyme, pH 7.2, 37°C
0.4
phosphoenolpyruvate
-
isozyme DS II, pH 7.5, 37°C
0.891
phosphoenolpyruvate
pH 7.0, 60°C
1
phosphoenolpyruvate
-
DAHP synthase-tyr, pH 7.0, 37°C
1.11
phosphoenolpyruvate
-
DAHP synthase-trp, pH 7.0, 37°C
2
phosphoenolpyruvate
Phe-sensitive isozyme, pH 6.8, 25°C
2.724
phosphoenolpyruvate
-
at 37°C
3.5
phosphoenolpyruvate
-
pH 6.4, 37°C
8.52
phosphoenolpyruvate
-
isozyme NCgl2098 DAHP synthase, in 50 mM Tris-HCl buffer, pH 7.5, at 30°C
12.25
phosphoenolpyruvate
-
N-terminal deletion mutant, 25°C, pH 6.4
21
phosphoenolpyruvate
Phe-sensitive isozyme, pH 6.8, 25°C
21
phosphoenolpyruvate
sensitive to metal ion
additional information
additional information
-
-
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
kinetics
-
additional information
additional information
-
mutants
-
additional information
additional information
-
mutants
-
additional information
additional information
-
pH-dependence
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics, kinetics in presence of inhibitors L-phe and L-Tyr
-
additional information
additional information
-
Michaelis-Menten kinetics, kinetics in presence of inhibitors L-phe and L-Tyr
-