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Literature summary for 2.5.1.54 extracted from

  • Schofield, L.R.; Anderson, B.F.; Patchett, M.L.; Norris, G.E.; Jameson, G.B.; Parker, E.J.
    Substrate ambiguity and crystal structure of Pyrococcus furiosus 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase: an ancestral 3-deoxyald-2-ulosonate-phosphate synthase? (2005), Biochemistry, 44, 11950-11962.
    View publication on PubMed
Search for more literature for 2.5.1.54

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant enzyme, in complex with phosphoenolpyruvate Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: phenylalanine, tyrosine, tryptophane Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 2-deoxy-D-erythrose 4-phosphate pH 6.8, 60°C Pyrococcus furiosus
0.009
-
 D-erythrose 4-phosphate pH 6.8, 60°C Pyrococcus furiosus
0.033
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate 2-deoxy-D-erythrose 4-phosphate Pyrococcus furiosus
0.035
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate 2-deoxy-D-ribose 5-phosphate Pyrococcus furiosus
0.036
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate D-ribose 5-phosphate Pyrococcus furiosus
0.062
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate D-arabinose 5-phosphate Pyrococcus furiosus
0.093
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate D-erythrose 4-phosphate Pyrococcus furiosus
1.58
-
 D-ribose 5-phosphate pH 6.8, 60°C Pyrococcus furiosus
2.5
-
 2-deoxy-D-ribose 5-phosphate pH 6.8, 60°C Pyrococcus furiosus
2.7
-
 D-arabinose 5-phosphate pH 6.8, 60°C Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U0A9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no substrate: D-glyceraldehyde 3-phosphate, D-glucose 6-phosphate Pyrococcus furiosus ?
-
 ?
phosphoenolpyruvate + 2-deoxy-D-erythrose 4-phosphate + H2O
-
 Pyrococcus furiosus 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
 ?
phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate + H2O
-
 Pyrococcus furiosus 3,5-dideoxy-D-gluco-octulosonate 8-phosphate + 3,5-dideoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?
phosphoenolpyruvate + D-arabinose 5-phosphate
-
 Pyrococcus furiosus 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
-
 ?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
-
 Pyrococcus furiosus 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
-
 ?
phosphoenolpyruvate + D-ribose 5-phosphate + H2O
-
 Pyrococcus furiosus 3-deoxy-D-altro-octulosonate 8-phosphate + phosphate
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.12
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate D-arabinose 5-phosphate Pyrococcus furiosus
1.4
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate D-erythrose 4-phosphate Pyrococcus furiosus
1.7
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate 2-deoxy-D-ribose 5-phosphate Pyrococcus furiosus
2.5
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate D-ribose 5-phosphate Pyrococcus furiosus
3
-
 phosphoenolpyruvate pH 6.8, 60°C, cosubstrate 2-deoxy-D-erythrose 4-phosphate Pyrococcus furiosus