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2.5.1.54: 3-deoxy-7-phosphoheptulonate synthase

This is an abbreviated version!
For detailed information about 3-deoxy-7-phosphoheptulonate synthase, go to the full flat file.

Word Map on EC 2.5.1.54

Reaction

phosphoenolpyruvate
+
D-erythrose 4-phosphate
 +
H2O
=
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate
 +
phosphate

Synonyms

2-dehydro-3-deoxy-D-arabino-heptonate-7-phosphate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating), 2-dehydro-3-deoxy-phosphoheptanoate aldolase, 2-dehydro-3-deoxy-phosphoheptonate aldolase, 2-keto-3-deoxy-D-arabino-heptonic acid 7-phosphate synthetase, 3-deoxy-7-phosphoheptulonate synthase, 3-deoxy-D-arabino heptulosonate 7-phosphate synthase, 3-deoxy-D-arabino-2-heptulosonic acid 7-phosphate synthetase, 3-deoxy-D-arabino-heptolosonate-7-phosphate synthetase, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase, 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase, 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase, 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase, 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating), 7-phospho-2-keto-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate lyase (pyruvate-phosphorylating), aldolase, phospho-2-keto-3-deoxyheptanoate, Aro1A, Aro4, Aro4p, AroA, AroA(Q), aroF, AroFFR, AroG, D-erythrose-4-phosphate-lyase, D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating), DAH7-P synthase, DAH7-P synthase (phe), DAH7P, DAH7P synthase, DAH7PS, DAHP synthase, DAHP synthase-phe, DAHP synthase-trp, DAHP synthase-tyr, DAHP(Phe), DAHPS, DAHPS(Tyr), Dax1, deoxy-D-arabino-heptulosonate-7-phosphate synthetase, DHS, DHS1, DS-Co, DS-Mn, EC 4.1.2.15, KDPH synthase, KDPH synthetase, More, mtDAH7Ps, MtDS, NCgl0950 DAHP synthase, NCgl2098 DAHP synthase, Nme-DAH7PS, OsDAHPS, PF1690, phospho-2-dehydro-3-deoxyheptonate aldolase, phospho-2-keto-3-deoxyheptanoate aldolase, Phospho-2-keto-3-deoxyheptonate aldolase, phospho-2-keto-3-deoxyheptonic aldolase, phospho-2-oxo-3-deoxyheptonate aldolase, phzC, TmaDAH7PS

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.54 3-deoxy-7-phosphoheptulonate synthase

General Stability

General Stability on EC 2.5.1.54 - 3-deoxy-7-phosphoheptulonate synthase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, presence of 1 mM dithiothreitol, stable
-
-80°C, stable for at least 2 months
-
37°C, 65 min, 50% residual activity
-
4°C, presence of 1 mM dithiothreitol, stabel for at least 24 h
-
Co2+ stabilizes
-
Cu2+ and Fe2+ accelerates subunit dissociation
-
dithiothreitol stabilizes during incubation at 30°C for 15 min
-
DTT stabilizes the active protein, no influence on the alkylated enzyme
-
loss of 50% activity by freezing and thawing the cell extract
-
metal-catalysed oxidation of the enzyme, the apoenzyme shows an exponentially decrease in activity with a half-life of about 1 day at 22°C, Cu2+ and Fe2+ accelerated the rate of inactivation and subunit dissociation, phosphoenolpyruvate and EDTA stabilize, mutants are insensitive
-
Mn2+ stabilizes during purification
-
overnight dialysis against buffer containing 10 mM EDTA at 4°C results in 85% inactivation
-
phosphoenolpyruvate stabilizes
-
resistant to denaturation by sodium dodecyl sulfate
-
spontaneous inactivation with a net loss of two of the seven thiol groups per subunit is restored by dithiothreitol
-
unstable during manipulations such as dialysis, dilution, ammonium sulfate fractionation, chromatography on DEAE-cellulose or Sephadex G-200
-