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Literature summary for 2.5.1.54 extracted from
- 2-Deoxy-D-arabino-heptulosonate-7-phosphate synthase of Streptomyces aureofaciens Tü 24 (1971), Biochim. Biophys. Acta, 242, 617-629.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | stimulates | Kitasatospora aureofaciens |  |
General Stability
| General Stability | Organism |
|---|---|
| dithiothreitol stabilizes during incubation at 30°C for 15 min | Kitasatospora aureofaciens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | reversible by Co2+ | Kitasatospora aureofaciens | |
| p-hydroxymercuribenzoate | - |
Kitasatospora aureofaciens | |
| Phe | no inhibition | Kitasatospora aureofaciens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
D-erythrose 4-phosphate | 30°C, pH 7.0 | Kitasatospora aureofaciens | |
| 0.3 | - |
phosphoenolpyruvate | 30°C, pH 7.0 | Kitasatospora aureofaciens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | stimulates | Kitasatospora aureofaciens | |
| Co2+ | required for full activity | Kitasatospora aureofaciens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 97000 | - |
gel filtration | Kitasatospora aureofaciens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Kitasatospora aureofaciens | first enzyme of the aromatic amino acid biosynthesis | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | Kitasatospora aureofaciens Tu24 | first enzyme of the aromatic amino acid biosynthesis | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kitasatospora aureofaciens | - |
strain Tü 24 | - |
| Kitasatospora aureofaciens Tu24 | - |
strain Tü 24 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 170fold | Kitasatospora aureofaciens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | ordered ping-pong bi-bi mechanism | Kitasatospora aureofaciens | |
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | first substrate is phosphoenolpyruvate | Kitasatospora aureofaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.3 | - |
partially purified enzyme | Kitasatospora aureofaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Kitasatospora aureofaciens | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | first enzyme of the aromatic amino acid biosynthesis | Kitasatospora aureofaciens | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Kitasatospora aureofaciens Tu24 | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
| phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | first enzyme of the aromatic amino acid biosynthesis | Kitasatospora aureofaciens Tu24 | 2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Kitasatospora aureofaciens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
phosphoenolpyruvate protects against inactivation | Kitasatospora aureofaciens |
| additional information | - |
D-erythrose 4-phosphate, erythrose and ribose 5-phosphate increase rate of inactivation | Kitasatospora aureofaciens |
| 30 | - |
pH 6.0-8.0, stable for at least 2 h | Kitasatospora aureofaciens |
| 50 | - |
inactivation above | Kitasatospora aureofaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Kitasatospora aureofaciens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 7.9 | pH 6.2: about 35% of maximal activity, pH 7.9: about 55% of maximal activity | Kitasatospora aureofaciens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | 30°C, stable for at least 2 h | Kitasatospora aureofaciens |
| 7 | 7.5 | maximal stability | Kitasatospora aureofaciens |
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Release 2023.1 (January 2023)
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