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2.5.1.54: 3-deoxy-7-phosphoheptulonate synthase

This is an abbreviated version!
For detailed information about 3-deoxy-7-phosphoheptulonate synthase, go to the full flat file.

Word Map on EC 2.5.1.54

Reaction

phosphoenolpyruvate
+
D-erythrose 4-phosphate
 +
H2O
=
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate
 +
phosphate

Synonyms

2-dehydro-3-deoxy-D-arabino-heptonate-7-phosphate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating), 2-dehydro-3-deoxy-phosphoheptanoate aldolase, 2-dehydro-3-deoxy-phosphoheptonate aldolase, 2-keto-3-deoxy-D-arabino-heptonic acid 7-phosphate synthetase, 3-deoxy-7-phosphoheptulonate synthase, 3-deoxy-D-arabino heptulosonate 7-phosphate synthase, 3-deoxy-D-arabino-2-heptulosonic acid 7-phosphate synthetase, 3-deoxy-D-arabino-heptolosonate-7-phosphate synthetase, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase, 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase, 3-deoxy-D-arabino-heptulosonic acid 7-phosphate synthase, 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase, 3-deoxy-D-arabinoheptulosonate-7-phosphate synthetase, 7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating), 7-phospho-2-keto-3-deoxy-D-arabino-heptonate D-erythrose-4-phosphate lyase (pyruvate-phosphorylating), aldolase, phospho-2-keto-3-deoxyheptanoate, Aro1A, Aro4, Aro4p, AroA, AroA(Q), aroF, AroFFR, AroG, D-erythrose-4-phosphate-lyase, D-erythrose-4-phosphate-lyase (pyruvate-phosphorylating), DAH7-P synthase, DAH7-P synthase (phe), DAH7P, DAH7P synthase, DAH7PS, DAHP synthase, DAHP synthase-phe, DAHP synthase-trp, DAHP synthase-tyr, DAHP(Phe), DAHPS, DAHPS(Tyr), Dax1, deoxy-D-arabino-heptulosonate-7-phosphate synthetase, DHS, DHS1, DS-Co, DS-Mn, EC 4.1.2.15, KDPH synthase, KDPH synthetase, More, mtDAH7Ps, MtDS, NCgl0950 DAHP synthase, NCgl2098 DAHP synthase, Nme-DAH7PS, OsDAHPS, PF1690, phospho-2-dehydro-3-deoxyheptonate aldolase, phospho-2-keto-3-deoxyheptanoate aldolase, Phospho-2-keto-3-deoxyheptonate aldolase, phospho-2-keto-3-deoxyheptonic aldolase, phospho-2-oxo-3-deoxyheptonate aldolase, phzC, TmaDAH7PS

ECTree

     2 Transferases
         2.5 Transferring alkyl or aryl groups, other than methyl groups
             2.5.1 Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
                2.5.1.54 3-deoxy-7-phosphoheptulonate synthase

Cloned

Cloned on EC 2.5.1.54 - 3-deoxy-7-phosphoheptulonate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 clones M1 and M2, expression in Escherichia coli strain BL21(DE3) and strain AB3248, which is defective in all endogenous isozyme forms, functional complementation of the mutant
-
ARO3 gene, expression in deficient strain and in Escherichia coli strain JA196
-
ARO4 gene, tyrosine-sensitive isozyme, overexpression from plasmid in strain RH1326
-
DHS1 and DHS2, complementation of Saccharomyces cerevisiae mutant strain YBK7
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli strain NSTCSRA
-
expressed in soluble form by co-expression with chaperonins GroEL/GroES in Escherichia coli
expression in Escherichia coli
expression of enzyme with affinity purification tagwith plasmid pKTDS-HN in Escherichia coli KA13
expression of mature chloroplastic form, without signal sequence, in Escherichia coli
-
gene aroF, cloning of mutant enzymes in Escherichia coli strain JM109, recombinant overexpression of mutant enzyme variants AroFD(1-9), AroFD(1-10), AroFD(1-12) and AroFD(1-11)
gene aroF, Tyr-sensitive isozyme, expression of wild-type and mutants in strain AB3257
-
gene aroF, Tyr-sensitive isozyme, overexpression in strain BL21(DE3)
-
gene aroG, Phe-sensitive isozyme, overexpression in strain gene aroH, Trp-sensitive isozyme, overexpression in deficient strain AB3248
-
gene aroG, recombinant expression of a truncated coding DNA sequence of aroG in transgenic Solanum lycopersicum cv. 82 fruits under control of a fruit ripening-specific promoter, E8. Metabolic profiling of transgenic tomato plants expressing a bacterial feedback-insensitive AroG gene, overview
gene aroG, recombinant expression of His10-tagged enzyme in Escherichia coli strain BL21, subcloning in Escherichia coli strain DH5alpha
gene aroG, recombinant expression of mutant enzyme R126S in Escherichia coli strain BL21 StarTM (DE3)
gene aroG, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain (DE3) Star
gene aroG, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3)
gene GhDHS1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, overexpressionin transgenic Arabidopsis thaliana plants via Agrobacterium tumefaciens-mediated transformation
gene Os07g0622200, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant functional expression in Escherichia coli aroH mutant strain ME8637 and complementating it
N-terminally histidine-tagged enzyme is expressed in Escherichia coli KA13
overexpression in BL21(DE3)
-
overexpression of 3 isozymes
-
overexpression of His-tagged wild-type and mutants in Escherichia coli
-
Phe-sensitive isozyme, wild-type and mutants, expression in Escherichia coli strain BL21(DE3)
-