General Stability | Organism |
---|---|
-70°C, presence of 1 mM dithiothreitol, stable | Helicobacter pylori |
4°C, presence of 1 mM dithiothreitol, stabel for at least 24 h | Helicobacter pylori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate | - |
Helicobacter pylori | |
EDTA | inactivation, reactivation by divalent metyl ions, in decreasing order: Co2+, Mn2+, Ca2+, Mg2+, Cu2+, Zn2+ | Helicobacter pylori | |
additional information | not inhibitory: phenylalanine, tyrosine, tryptophan, chorismate | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
phosphoenolpyruvate | pH 7.5, 30°C | Helicobacter pylori | |
0.006 | - |
D-erythrose 4-phosphate | pH 7.5, 30°C | Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | reactivation of EDTA-treated enzyme by divalent metyl ions, in decreasing order: Co2+, Mn2+, Ca2+, Mg2+, Cu2+, Zn2+ | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
x * 51000, SDS-PAGE | Helicobacter pylori |
100000 | - |
gel fitlration | Helicobacter pylori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Helicobacter pylori |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | ordered sequential mechanism | Helicobacter pylori |
Renatured (Comment) | Organism |
---|---|
reactivation of EDTA-treated enzyme by divalent metyl ions, in decreasing order: Co2+, Mn2+, Ca2+, Mg2+, Cu2+, Zn2+ | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | reaction involves interaction of si face of phosphoenolpyruvate with re face of eryxthrose 4-phosphate | Helicobacter pylori | ? | - |
? | |
phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate + H2O | - |
Helicobacter pylori | 3,5-dideoxy-D-gluco-octulosonate 8-phosphate + 3,5-dideoxy-D-manno-octulosonate 8-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O | - |
Helicobacter pylori | 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate | - |
? | |
phosphoenolpyruvate + D-ribose 5-phosphate + H2O | - |
Helicobacter pylori | 3-deoxy-D-altro-octulosonate 8-phosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x * 51000, SDS-PAGE | Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
phosphoenolpyruvate | pH 7.5, 30°C | Helicobacter pylori |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate | pH 7.5, 30°C, cosubstrate phosphoenolpyruvate | Helicobacter pylori | |
1.5 | - |
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate | pH 7.5, 30°C, cosubstrate erythrose 4-phosphate | Helicobacter pylori |