Information on EC 2.5.1.54 - 3-deoxy-7-phosphoheptulonate synthase

Word Map on EC 2.5.1.54
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota

EC NUMBER
COMMENTARY hide
2.5.1.54
-
RECOMMENDED NAME
GeneOntology No.
3-deoxy-7-phosphoheptulonate synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phospate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aldol condensation
condensation
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-dehydroquinate biosynthesis I
-
-
Biosynthesis of antibiotics
-
-
Biosynthesis of secondary metabolites
-
-
Metabolic pathways
-
-
Phenylalanine, tyrosine and tryptophan biosynthesis
-
-
chorismate metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
phosphoenolpyruvate:D-erythrose-4-phosphate C-(1-carboxyvinyl)transferase (phosphate-hydrolysing, 2-carboxy-2-oxoethyl-forming)
-
CAS REGISTRY NUMBER
COMMENTARY hide
9026-94-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
strain U-32; Trp-sensitive isozyme
-
-
Manually annotated by BRENDA team
strain U-32
-
-
Manually annotated by BRENDA team
wild type strain WV2 contains DS I, a leaky L-Phe-requiring auxotroph mutant strain GH141 grown under L-Phe limitation possesses additional DS II activity
-
-
Manually annotated by BRENDA team
2 isozymes: DS-Co and DS-Mn; cv. JL-24
-
-
Manually annotated by BRENDA team
strain B-6
-
-
Manually annotated by BRENDA team
strain B-6
-
-
Manually annotated by BRENDA team
strain 12/60/X, strain 33/X; strain3/2; strain H1; strain H20
-
-
Manually annotated by BRENDA team
Bacteria H1
strain H1
-
-
Manually annotated by BRENDA team
Bacteria H20
strain H20
-
-
Manually annotated by BRENDA team
Bacteria strain3/2
strain3/2
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain ATCC 17409
-
-
Manually annotated by BRENDA team
basonym Alcaligenes eutrophus; strain H16
-
-
Manually annotated by BRENDA team
strain H16
-
-
Manually annotated by BRENDA team
i.e. Comamonas terrigena; strain ATCC 11299a, two distinct regulatory isoenzymes: DAHP synthase-phe and DAHP synthase-tyr
-
-
Manually annotated by BRENDA team
strain HE 401
-
-
Manually annotated by BRENDA team
strain NST37 (NST, ATCC31882)
-
-
Manually annotated by BRENDA team
strain W3110
-
-
Manually annotated by BRENDA team
cv. Jimian20, gene GhDHS1
UniProt
Manually annotated by BRENDA team
strain ATCC 17724
-
-
Manually annotated by BRENDA team
serotype B, gene aroG
UniProt
Manually annotated by BRENDA team
strain 74-OR23-1A
-
-
Manually annotated by BRENDA team
strain 74A
-
-
Manually annotated by BRENDA team
cytosolic isozyme
-
-
Manually annotated by BRENDA team
no activity in Homo sapiens
-
-
-
Manually annotated by BRENDA team
plastidic isozyme DS-Mn and cytosolic isozyme DS-Co
-
-
Manually annotated by BRENDA team
2 isoenzymes: tyrosine-sensitive DAHP synthase-tyr and tryptophane-sensitive DAHP synthase-trp
-
-
Manually annotated by BRENDA team
strain RH1326
-
-
Manually annotated by BRENDA team
tyrosine-sensitive isozyme
-
-
Manually annotated by BRENDA team
cytosolic isozyme Ds-Co
-
-
Manually annotated by BRENDA team
strain Tü 24
-
-
Manually annotated by BRENDA team
strain Tü 24
-
-
Manually annotated by BRENDA team
strain A3(2); Trp-sensitive isozyme
-
-
Manually annotated by BRENDA team
strain A3(2)
-
-
Manually annotated by BRENDA team
strain 7C
-
-
Manually annotated by BRENDA team
strain 7C
-
-
Manually annotated by BRENDA team
2 classes of enzymes: class AroAI and AroAII; strain ATCC 33436
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
metabolism
physiological function
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
phosphoenol-3-fluoropyruvate + D-erythrose 4-phosphate + H2O
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
-
enzyme does not discriminate between (E) and (Z)-form of phosphoenol-3-fluoropyruvate
-
-
?
phosphoenolpyruvate + (3S)-2-deoxyerythrose 4-phosphate + H2O
(5S)-[5H]-3,5-dideoxy-D-arabinoheptulosonate 7-phosphate + phosphate
show the reaction diagram
-
-
-
-
ir
phosphoenolpyruvate + 2-deoxy-D-erythrose 4-phosphate + H2O
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
-
-
-
?
phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate
3,5-dideoxy-D-gluco-octulosonate 8-phosphate + 3,5-dideoxy-D-manno-octulosonate 8-phosphate + phosphate
show the reaction diagram
-
-
-
?
phosphoenolpyruvate + 2-deoxy-D-ribose 5-phosphate + H2O
3,5-dideoxy-D-gluco-octulosonate 8-phosphate + 3,5-dideoxy-D-manno-octulosonate 8-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D,L-lyxose
2-dehydro-3-deoxy-D,L-galacto-octonate + phosphate
show the reaction diagram
-
D-lyxose or L-lyxose, at 1.8% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-arabinose
2-dehydro-3-deoxy-D-gluco-octonate + phosphate
show the reaction diagram
-
at 0.7% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-arabinose 5-phosphate
3-deoxy-D-manno-octulosonate 8-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-erythrose
2-dehydro-3-deoxy-D-arabino-heptonate + phosphate
show the reaction diagram
-
at 93% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
3-deoxy-D-arabino-hept-2-ulosonate 7-phospate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-fructose 1,6-diphosphate
?
show the reaction diagram
-
same activity as with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-fructose 6-phosphate
?
show the reaction diagram
-
same activity as with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-glucose 6-phosphate
?
show the reaction diagram
phosphoenolpyruvate + D-glyceraldehyde
2-dehydro-3-deoxy-D-threo-hexonate + phosphate
show the reaction diagram
-
at 176% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-ribose 5-phosphate
3-deoxy-D-altro-octulosonate 8-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-ribose 5-phosphate + H2O
3-deoxy-D-altro-octulosonate 8-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-ribulose 5-phosphate
?
show the reaction diagram
-
at 12% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-threose
2-dehydro-3-deoxy-D-xylo-heptonate + phosphate
show the reaction diagram
-
at 92% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-xylose
2-dehydro-3-deoxy-D-ido-octonate + phosphate
show the reaction diagram
-
at 1% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + D-xylulose 5-phosphate
?
show the reaction diagram
phosphoenolpyruvate + DL-glyceraldehyde 3-phosphate
pyruvate + phosphate
show the reaction diagram
phosphoenolpyruvate + erythrose 4-phosphate + H2O
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + glycolaldehyde
(S)-2-oxo-4,5-dihydroxypentanoate + phosphate
show the reaction diagram
-
at 245% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + glyoxylate
S-4-hydroxy-2-oxo-1,5-pentanedioate + R-4-hydroxy-2-oxo-1,5-pentanedioate + phosphate
show the reaction diagram
-
at 205% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + L-erythrose
2-dehydro-3-deoxy-L-arabino-heptonate + phosphate
show the reaction diagram
-
at 70% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + L-glyceraldehyde
2-dehydro-3-deoxy-L-threo-hexonate + phosphate
show the reaction diagram
-
at 212% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + L-threose
2-dehydro-3-deoxy-L-xylo-heptonate + phosphate
show the reaction diagram
-
at 52% of the activity with D-erythrose 4-phosphate
-
-
?
phosphoenolpyruvate + L-xylose
2-dehydro-3-deoxy-L-ido-octonate + phosphate
show the reaction diagram
-
at 1% of the activity with D-erythrose 4-phosphate
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
2-dehydro-3-deoxy-D-arabino-heptonate 7-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
3-deoxy-D-arabino-hept-2-ulosonate 7-phospate + phosphate
show the reaction diagram
phosphoenolpyruvate + D-erythrose 4-phosphate + H2O
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
phosphoenolpyruvate + erythrose 4-phosphate + H2O
3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate
show the reaction diagram
additional information
?
-
-
structure comparison between 3-deoxy-7-phosphoheptulonate synthase and 3-deoxy-D-manno-octulosonate 8-phosphate synthase, EC 4.1.2.16, reveal that they share a common ancestor and adopt the same catalytic strategy
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
0.1 mM, up to 500% of initial activity
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1R,2S)-1,2-epoxypropylphosphonic acid
-
-
(2R)-2-(phosphonooxy)propanoic acid
-
mimicking phosphohemiketal 2 (instable), competitive to substrate phosphoenolpyruvate
(2S)-2,7-bis(phosphonooxy)heptanoic acid
-
-
(2S)-2-(phosphonooxy)propanoic acid
-
mimicking phosphohemiketal 2 (instable), competitive to substrate phosphoenolpyruvate
(2Z)-3-phosphono-2-(trifluoromethyl)prop-2-enoic acid
-
trifluorinated phosphonate
(E)-2-methyl-3-phosphonoacrylic acid
-
most potent of the tested inhibitors mimicking intermediates in the reaction, vinyl phosphonate 4
(R)-phospholactate
-
-
-
(S)-phospholactate
-
-
1,10-phenanthroline
-
activity is restored by Fe2+ or Zn2+
2,3-bisphosphoglycerate
-
-
2-(phosphonomethyl)prop-2-enoic acid
-
mimics substrate phosphoenolpyruvate, and should be inert due to alkene structure but lacking an electron-donor, acts as competitive inhibitor
2-Methyl-DL-Trp
-
0.02 mM, 30% inhibition
2-phosphoglycerate
-
competitive with respect to phosphoenolpyruvate
3,4-dihydroxycinnamate
-
isoenzyme DS-Co
3-deoxy-D-arabino-heptonic acid 7-phosphate
-
-
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate
-
-
3-Methylphosphoenolpyruvate
-
-
3-Propylphosphoenolpyruvate
-
-
3-pyridine carboxyaldehyde
-
-
4- allylpyrocatechol
-
-
4-Methyl-DL-Trp
-
0.02 mM, 30% inhibition
5,5'-dithiobis(2-nitrobenzoate)
-
-
5-Fluoro-DL-Trp
-
0.02 mM, 56% inhibition
5-hydroxy-DL-Trp
-
0.02 mM, 10% inhibition
6-Methyl-DL-Trp
-
0.02 mM, 6% inhibition
7-Aza-DL-Trp
-
0.02 mM, 9% inhibition
7-Methyl-DL-Trp
-
0.02 mM, 14% inhibition
7-O-methylwogonin
-
-
7-phospho-2-dehydro-3-deoxy-D-arabino-heptonate
allylpyrocatechol-3,4-diacetate
-
-
alpha-Methylphenylalanine
-
-
beta-2-Thienyl-D,L-Ala
-
-
beta-phenylserine
-
-
beta-Thienylalanine
-
-
chorismate
CN-
-
Tyr-sensitive isozyme, strong inhibition, reactivation by divalent cations only to a small extent
D-erythrose 4-phosphate
D-fructose 1,6-diphosphate
-
-
D-sedoheptulose 1,7-diphosphate
-
-
D-sedoheptulose 7-phosphate
-
-
diethyl dicarbonate
-
Phe-sensitive isozyme, pH-dependent, phosphoenolpyruvate protects wild-type and mutants H64G, H207G, H304G
dihydroxyphenylalanine
-
-
dipicolinic acid
DL-Dibromotryptophan
-
0.02 mM, 52% inhibition
DL-erythro-beta-Methyltryptophan
-
0.02 mM, 40% inhibition
DL-Homotryptophan
-
0.02 mM, 46% inhibition
eupalitin-3-O-galactoside
-
-
fosmidomycin
-
uncompetitive inhibitor, maximum level of inhibition after 10 min incubation, extent of inhibition dependent on the type of the metal cofactor, competitive inhibitor with respect to phosphoenolpyruvate
H2O2
-
DAHP synthase enzymes are inactivated by H2O2 in vitro and in vivo, H2O2 displaces the iron atom from the enzyme, only the Fe2+-metalloform of the enzyme can be inactivated by hydrogen peroxide or superoxide
Hg2+
-
complete inhibition at 1 mM
iodoacetamide
-
alkylation, abolishes the dependence on reducing agents
L-arogenate
-
isozyme DS-Mn, competitive against D-erythrose 4-phosphate, non-competitive against phosphoenolpyruvate
L-Phe
L-phenylalanine
L-Trp
L-tryptophan
L-Tyr
L-tyrosine
m-Chlorophenylalanine
-
-
m-Fluorophenylalanine
-
-
m-hydroxyphenylalanine
-
-
Metal chelators
-
-
-
Mg2+
-
no inhibition of mutant C67L; wild-type and mutants
N-alpha-methyl-DL-Trp
-
0.02 mM, 60% inhibition
N-bromosuccinimide
-
-
N-ethylmaleimide
-
-
N-[Phosphomonomethyl]glycine
Na2SO4
-
slightly
o-Chlorophenylalanine
-
-
o-Fluorophenylalanine
-
-
o-Hydroxyphenylalanine
-
-
p-aminophenylalanine
-
-
p-chloromercuribenzoate
-
complete inhibition at 0.02 mM, reversible by cysteine
p-Fluorophenylalanine
-
-
p-hydroxymercuribenzoate
-
-
phenylalanine
phenylpyruvate
-
DAHP synthase-tyr
phosphate
phosphoenolpyruvate
pronounced substrate inhibition above 1 mM
prephenate
shikimate
-
1 mM, 3% residual activity
superoxide
-
displaces the iron atom from the enzyme, only the Fe2+-metalloform of the enzyme can be inactivated by hydrogen peroxide or superoxide. Superoxide stress promotes the mismetallation of DAHP synthase
tetraammonium (((carboxymethyl)[(2S,3R,4S)-2,3,4-trihydroxy-5-(phosphonatooxy)pentyl]amino)methyl)phosphonate
-
IC50: 0.0066 mM
Tetranitromethane
-
-
Trinitrobenzene sulfonate
-
-
tyrosine
vinyl phosphonate
-
-
-
[(1E)-7-bromo-2-carboxyhept-1-en-1-yl]phosphonate
-
inhibitor based on vinyl phosphonate, designed to fit into the binding sites of both phosphoenolpyruvate and D-erythrose 4-phosphate substrates simultaneously. Competitive with respect to phosphoenolpyruvate
[2-carboxy-7-(phosphonatooxy)hept-1-en-1-yl]phosphonate
-
inhibitor based on vinyl phosphonate ratio Z:E enantiomer 1:1. Inhibitor is designed to fit into the binding sites of both phosphoenolpyruvate and D-erythrose 4-phosphate substrates simultaneously. Competitive with respect to phosphoenolpyruvate
[2-carboxy-7-(phosphonatooxy)hept-2-en-1-yl]phosphonate
-
inhibitor based on allyl phosphonate, ratio Z:E enantiomer 7:3. Inhibitor is designed to fit into the binding sites of both phosphoenolpyruvate and D-erythrose 4-phosphate substrates simultaneously. Competitive with respect to phosphoenolpyruvate
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
activates
beta-mercaptoethanol
-
-
Chorismic acid
dithiothreitol
L-Trp
N-[Phosphomonomethyl]glycine
-
i.e. glyphosate; induces synthesis of enzyme
Phe
-
slightly activating, mutants S187Y, S187F, S187C
reduced thioredoxin
-
required for activity, can be partially substituted by dithiothreitol
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.65
(3S)-2-deoxyerythrose 4-phosphate
-
pH 6.8
0.006
2-deoxy-D-erythrose 4-phosphate
-
pH 6.8, 60°C
2.5
2-deoxy-D-ribose 5-phosphate
-
pH 6.8, 60°C
6.8
2-deoxyribose 5-phosphate
-
recombinant enzyme, pH 7.5, 25°C
0.03 - 2.7
D-arabinose 5-phosphate
2.5
D-erythrose
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
0.0026 - 52.36
D-erythrose 4-phosphate
3.5
D-glyceraldehyde
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
1.58
D-ribose 5-phosphate
-
pH 6.8, 60°C
8.4
D-threose
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
3
DL-glyceraldehyde 3-phosphate
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
0.006
erythrose 4-phosphate
-
pH 7.5, 30°C
8.6
glycolaldehyde
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
3.6
glyoxylate
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
5.1
L-erythrose
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
3.3
L-Glyceraldehyde
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
13.9
L-threose
-
cytosolic isozyme DS-Co, pH 8.6, 37°C
0.003 - 21
phosphoenolpyruvate
6
ribose 5-phosphate
-
recombinant enzyme, pH 7.5, 25°C
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.233
(3S)-2-deoxyerythrose 4-phosphate
Escherichia coli
-
pH 6.8
0.03 - 122
D-erythrose 4-phosphate
0.0035 - 122
phosphoenolpyruvate
additional information
additional information
Corynebacterium glutamicum
-
mutants
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.069 - 940
D-erythrose 4-phosphate
500
0.22 - 2400
phosphoenolpyruvate
51
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.049
(2R)-2-(phosphonooxy)propanoic acid
-
buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate
0.67
(2S)-2-(phosphonooxy)propanoic acid
-
buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate
0.0088
(2Z)-3-phosphono-2-(trifluoromethyl)prop-2-enoic acid
-
buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate
0.0047
(E)-2-methyl-3-phosphonoacrylic acid
-
buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate
0.099
(R)-phospholactate
-
pH 6.8, 25°C, recombinant enzyme
-
0.36
(S)-phospholactate
-
pH 6.8, 25°C, recombinant enzyme
0.27
2-(phosphonomethyl)prop-2-enoic acid
-
buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate
0.27 - 1.5
3-deoxy-D-erythro-hept-2-ulosonate 7-phosphate
1.35 - 2.25
chorismate
0.035
fosmidomycin
-
buffer bis-tris-propane, 50 mM, pH 6.8, Mn2+, UV-visible spectroscopy at 232 nm, substrates phosphoenolpyruvate and D-erythrose 4-phosphate
0.01 - 0.27
Phe
1.35 - 2.55
phenylpyruvate
22 - 54
phosphate
0.005 - 0.04
Trp
0.0009 - 0.023
Tyr
0.0039
vinyl phosphonate
-
pH 6.8, 25°C, recombinant enzyme
-
additional information
additional information
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.054
3-pyridine carboxyaldehyde
Mycobacterium tuberculosis
O53512
pH and temperature not specified in the publication
-
0.021
alpha-tocopherol
Mycobacterium tuberculosis
O53512
pH and temperature not specified in the publication
1
chorismate
Bacillus subtilis
-
IC50: 1 mM
0.1
prephenate
Bacillus subtilis
-
IC50: 0.1 mM
0.042
rutin
Mycobacterium tuberculosis
O53512
pH and temperature not specified in the publication
0.0066
tetraammonium (((carboxymethyl)[(2S,3R,4S)-2,3,4-trihydroxy-5-(phosphonatooxy)pentyl]amino)methyl)phosphonate
Escherichia coli