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2.3.1.20: diacylglycerol O-acyltransferase

This is an abbreviated version!
For detailed information about diacylglycerol O-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.20

Reaction

acyl-CoA
+
1,2-diacyl-sn-glycerol
=
CoA
 +
triacylglycerol

Synonyms

1,2-diacylglycerol acyltransferase, 2 diacylglycerol acyltransferase, ACY99349, acyl CoA:1,2-diacylglycerol acyltransferase, acyl CoA:diacylglycerol acyltransferase, acyl CoA:diacylglycerol acyltransferase 1, acyl CoA:diacylglycerol acyltransferase-2, acyl coenzyme A:diacylglycerol acyltransferase, acyl coenzyme A:diacylglycerol acyltransferase 1, acyl coenzyme A:diacylglycerol acyltransferase 2, acyl-CoA-dependent diacylglycerol acyltransferase, acyl-CoA: diacylglycerol acyltransferase, acyl-CoA:1,2-diacylglycerol O-acyltransferase, acyl-CoA:1,2-dioleoyl-sn-glycerol acyltransferase, acyl-CoA:diacylglycerol acyltransferase, acyl-CoA:diacylglycerol acyltransferase 1, acyl-CoA:diacylglycerol acyltransferase 2, acyl-CoA:diacylglycerol acyltransferase-2, acyl-CoA:diacylglycerol O-acyltransferase1, acyl-coenzyme A:diacylglycerol acyltransferase, acyltransferase, diacylglycerol, AtDGAT1A, AtDGAT3, AtfA, AtfG25, bifunctional DGAT/wax ester synthase, bifunctional wax synthase/DGAT, bifunctional WS/DGAT, BjDGAT1, BjDGAT2, BnaC.DGAT1.a, BnaDGAT1, BnaDGAT1.a, BnaDGAT1.b, BstDGAT1, CaDGAT1, CeDGAT1, CeDGAT2a, CeDGAT2b, CoDGAT2, CpuDGAT1, CrDGTT1, CrDGTT2, CrDGTT3, CsDGAT1B, CzDGAT1B, CzDGAT2, CzDGAT2A, CzDGAT2B, CzDGAT2C, CzDGAT2D, CzDGAT2E, CzDGAT2F, CzDGAT2G, DAG acyltransferase, DAGAT, DC3, DGA1, Dga1p, DGA2, DGAT, DGAT 1, DGAT 2, DGAT type 2, DGAT-1, DGAT-2, DGAT1, DGAT1-2, DGAT1-4, DGAT1-type enzyme, DGAT1.a, DGAT1A, DGAT1B, DGAT2, DGAT2-1, DGAT2.1, DGAT2.2, DGAT2.3, DGAT2A, DGAT2b, DGAT3, DGTT, diacylglycerol acyltransferase, diacylglycerol acyltransferase 1, diacylglycerol acyltransferase 2, diacylglycerol acyltransferase 3, diacylglycerol acyltransferase type 1, diacylglycerol acyltransferase type 2, diacylglycerol acyltransferase-1, diacylglycerol acyltransferase-2, diacylglycerol acyltransferase1, diacylglycerol acyltransferase2, diacylglycerol O-acyltransferase 1A, diacylglycerol O-acyltransferase 2, diacylglycerol O-acyltransferase 2D, diacylglycerol O-acyltransferase type-1, diacylglycerol O-acyltransferase-1, diglyceride acyltransferase, diglyceride O-acyltransferase, dual-function diacylglycerol acyltransferase, EC 2.3.1.124, ElDGAT1A, FBPA, Gat1, GmDGAT1A, GmDGAT1B, GmDGAT2D, GmDGAT3, HpDGAT2A, HpDGAT2B, HpDGAT2D, HpDGAT2E, Ma2, MaDGAT, MaDGAT2, MFAT, MGAT, mMaDGAT2, monoacylglycerol acyltransferase, More, MtDGAT1, multifunctional O-acyltransferase, NeoDGAT2, palmitoyl-CoA-sn-1,2-diacylglycerol acyltransferase, PDAT, PtDGATX, PtWS/DGAT, RtDGATa, RtDGATb, Rv3088 (TGS4), Rv3130c (TGS1), Rv3234c (TGS3), Rv3371, Rv3734 (TGS2), SAV7256, SCO0958, seed-specific DGAT1, SiDGAT1, SiDGAT2, TAG synthase, Tcur_3818, tDGAT, TmDGAT1, triacylglycerol:acyl-CoA:diacylglycerol acyltransferase, TrWSD4, TrWSD5, type 1 acyl-CoA: diacylglycerol acyltransferase, type 1 acyl-CoA:diacylglycerol acyltransferase, type 1 DGAT, type 1 diacylglycerol acyltransferase, type 2 acyl-CoA:diacylglycerol acyltransferase, type 2 DGAT, type 2 diacylglycerol acyltransferase, type I diacylglycerol acyltransferase, type II diacylglycerol acyltransferase, type-2 diacylglycerol acyltransferase, unspecific bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, VgDGAT1A, VgDGAT1B, wax ester synthase/acyl CoA:diacylglycerol acyltransferase, wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, wax ester synthase/acyl-CoA diacylglycerol acyltransferase, wax ester synthase/acyl-CoA:diacylglycerol acyltransferase, wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase WSD1, wax ester synthase/triacylglycerol:acylCoA acyltransferase, Wdh3563-1, Wdh3563-2, Wdh3563-3, Wdh3563-4, Wdh3563-7, WS/DGAT, WSD1, WSD4, WSD5, YALI0D07986g, YALI0E32769g

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.20 diacylglycerol O-acyltransferase

Cloned

Cloned on EC 2.3.1.20 - diacylglycerol O-acyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a single locus is identified that contains the gene encoding BstDGAT1, whose expression is highly cold-responsive, identification of the SKI (QTL seedling koude intolerance) locus in Boechera stricta, quantitative enzyme expression analysis
-
chromosomal location, expression of the FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells and in COS-7 cells
-
DGAT1, a single copy gene, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional overexpression in tobacco plants
DGAT1, DNA and amino acid sequence determination and analysis
DGAT1, DNA and amino acid sequence determination and analysis, phylogenetic tree
DGAT1, functional expression of the FLAG-tagged enzyme in membranes of Spodoptera frugiperda Sf9 cells and in COS-7 cells, expression analysis
DGAT2, DNA and amino acid sequence determination and analysis, DGAT2 is a truncated enzyme version, phylogenetic tree
DGAT2, functional expression of the FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells, expression analysis
DGATsv, splice variant, deletion of 101 residues from the C-terminus, enzymatically inactive
-
DNA and amino acid sequence determination and analysis
-
DNA and amino acid sequence determination and analysis, phylogenetic tree
-
DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in insect cells
expressed in a baculovirus expression system
expressed in a Saccharomyces cerevisiae H1246MATa quadruple mutant that is defective in triacylglycerol synthesis
-
expressed in Arabidopsis thaliana and Saccharomyces cerevisiae H1246 triacylglycerol-deficient mutant
expressed in Chlamydomonas reinhardtii strain CC-125
expressed in Escherichia coli
expressed in Escherichia coli and Saccharomyces cerevisiae
expressed in Escherichia coli C41(DE3) cells
Marinobacter nauticus
-
expressed in Escherichia coli Rosetta (DE3) cells
expressed in HEK-293 cells
-
expressed in HEK-293T cells and COS-7 cells
-
expressed in HEK-293T cells, RH-7777 cells, and COS-7 cells
-
expressed in Nicotiana benthamiana leaves
-
expressed in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae H1246 cells
-
expressed in Saccharomyces cerevisiae mutant H1246
expressed in Saccharomyces cerevisiae mutant H1246 and in soybean hairy roots
expressed in Saccharomyces cerevisiae mutant strain H1246
expressed in Saccharomyces cerevisiae strain CWY3626
expressed in Saccharomyces cerevisiae strain H1246
expressed in Saccharomyces cerevisiae strain InvSc1
expressed in Sf9 insect cells
-
expression as His-tagged enzyme in Escherichia coli strain BL21 (DE3), phylogenetic analysis
expression as soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression in baculoviral system
expression in HEK-293T cell
expression in Mycobacterium smegmatis
expression in Sf9 cell
-
expression of Myc-tagged DGAT1 and DGAT2 in Saccharomyces cerevisiae, co-expression with N- and C-terminally myc-tagged DGAT1 or DGAT2, and GFP-tagged DGAT2 in Nicotiana tabacum BY-2 cells in endoplasmic reticulum membranes oriented toward the cytosolic side
expression of Myc-tagged DGAT1 in Saccharomyces cerevisiae, co-expression with N- and C-terminally myc-tagged DGAT1
expression of Myc-tagged DGAT2 in Saccharomyces cerevisiae, co-expression with N- and C-terminally myc-tagged DGAT2, and GFP-tagged DGAT2 in Nicotiana tabacum BY-2 cells in endoplasmic reticulum membranes oriented toward the cytosolic side
expression of the poly-His-tagged N-terminal fragment of the enzyme, i.e. BnDGAT1(1–116)His6, in Escherichia coli strain BL21(DE3), BnDGAT1(1–116)His6 fails to react with protein in microsomal vesicles following treatment with proteinase K, and thus might be localized to the cytosolic side of the ER
-
gene atfG25, DNA and amino acid sequence determination and analysis, geneomic structure, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain C41(DE3)
-
gene BnaDGAT1.a, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme BnaDGAT1.a in Saccharomyces cerevisiae strain H1246, deficient in TAG synthesis
gene BnaDGAT1.b, sequence comparisons and phylogenetic analysis, recombinant expression of tagged enzyme BnaDGAT1.a in Saccharomyces cerevisiae strain H1246, deficient in TAG synthesis
gene CoDGAT2, sequence comparisons, recombinant expression in and complementation of Saccharomyces cerevisiae strain H1246 that is deficient in trinacylglycerol (TAG) production. Seed-specific overexpression of CoDGAT2 in Arabidopsis thaliana leads to a significant increase in the linoleic acid (C18:2) content (approximately 6%) compared to wild-type. In contrast, the proportions of eicosadienoic acid (C20:1) and arachidic acid (C20:0) are decreased, quantitative RT-PCR expression analysis
-
gene DC3, location of DC3 on the X chromosome at Xq13.1, DNA and amino acid sequence determination and analysis, expression analysis, expression in Saccharomyces cerevisiae
gene DGAT, genetic organization, DNA and amino acid sequence dteermination and analysis
gene DGAT1, BnaDGAT1 is recombinantly expressed using a codon-optimized cDNA in Saccharomyces cerevisiae strain H1246 microsomes, recombinant expression of truncated enzyme mutants in Saccharomyces cerevisiae strain H1246 microsomes
gene DGAT1, DNA and amino acid sequence determination and analysis, expression analysis of DGAT1 and DGAT2, expression in Saccharomyces cerevisiae microsomes, phylogenetic analysis
-
gene DGAT1, DNA and amino acid sequence determination and analysis, sequence comparison, RT-PCR expression analysis, recombinant expression in seeds of Camelina sativa. Microsomes of Camelina sativa seeds engineered for CpuDGAT1 expression display DGAT activity with 10:0-CoA and the diacylglycerol didecanoyl, that is approximately 4fold higher than that in camelina seed microsomes without CpuDGAT1, coexpression with a Cuphea viscosissima FatB thioesterase (CvFatB1) that generates 10:0 results in TAGs with nearly 15 mol% of 10:0. Recombinant expression of CpuDGAT1 and CvFatB1 with CvLPAT2, a 10:0-CoA-specific Cuphea LPAT, increases 10:0 amounts to 25 mol% in camelina seed TAG. These TAGs contain up to 40 mol% 10:0 in the sn-2 position, nearly double the amounts obtained from coexpression of CvFatB1 and CvLPAT2 alone. Although enriched in diacylglycerol, 10:0 is not detected in phosphatidylcholine in these seeds. Recombinant functional expression of CpuDGAT1 in the TAG-deficient Saccharomyces cerevisiae mutant strain H1246. CpuDGAT1 is able to rescue lipotoxicity of Saccharomyces cerevisiae H1246 cells grown in exogenous 0.25 mM C8:0 or C10:0, but not C18:1
gene DGAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, semiquantitative RT-PCR enzym expression analysis, functional recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, lipid analysis, overview
gene DGAT1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli and Saccharomyces cerevisiae double null strain DELZTADGA1/DELTALRO1, recombinant expression of mutant CaDGAT1 in Glycine max
gene DGAT1, quantitative RT-pCR expression analysis
gene DGAT1, recombinant expression of N-terminally His-tagged enzyme cytoplasmic and autoinhibitory domains, BnaDGAT11-113 and BnaDGAT11-80, in Escherichia coli strain BL21(DE3), method optimization and evaluation, overview. The tag is located at the disordered N-terminal region, which is distal to the folded C-terminal region and the primary ligand binding site. Hence tag-induced secondary structure elements, if any, might not influence the ligand binding properties
gene DGAT1, recombinant expression of the isozyme in Spodoptera frugiperda Sf9 cells via baculovirus transfection method
gene DGAT1, seed-specific co-overexpression of AtDGAT1 and ScGPD1 codon-optimized genes in Camelina sativa cv. Suneson, increasing seed mass, seed size and seed yield in the transgenic plants, recombinant co-expression also with mutant DGATm, the glycinin promoter from Glycine max is selected to drive the expression of DGAT1, transfection using the Agrobacterium tumefaciens strain GV3101 method. Quantitative real-time PCR enzyme expression analysis and phenotypes, overview
gene DGAT1, sequence comparisons, recombinant expression of wild-type and mutant enzymes, recombinant expression in Escherichia coli and Saccharomyces cerevisiae double null strain DELZTADGA1/DELTALRO1
gene DGAT1, single copy gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis of DGAT isozymes, recombinant expression in the TAG-deficient Saccharomyces cerevisiae strain H1246, LiDGATs of type 1 and 2 do not co-localize with each other at least in yeast
gene DGAT1, transient expression of N-terminally truncated CzDGAT1 variants and of the ACBP-fused versions in Nicotiana benthamiana leaves for lipid production using the transformation method via Agrobacterium tumefaciens GV3101 cells
gene DGAT1A, functional recombinant expression in Saccharomyces cerevisiae mutant strain H1246 and in Saccharomyces cerevisiae wild-type strain YPH499a, isozyme GmDGAT1A complements the TAG deficiency of mutant strain H1246. Recombinant expression in Arabidopsis thaliana using the Agrobacterium tumefaciens GV3101 transfection method. Quantitative RT-PCR enzyme expression analysis. Determination of the subcellular localization of GmDGAT-GFP performed by using tobacco leaf infiltration
gene DGAT1A, recombinant expression of isozyme AtDGAT1A in Saccharomyces cerevisiae strain INVSc1 microsomes, very low expression level, lipid analyses, overview
gene DGAT1A, recombinant expression of isozyme ElDGAT1A in Spodoptera frugiperda Sf9 insect cells via recombinant baculovirus transfection method, lipid analyses, overview
gene DGAT1A, recombinant expression of isozyme GmDGAT1A in Saccharomyces cerevisiae strain INVSc1 microsomes, and in Spodoptera frugiperda Sf9 insect cells via recombinant baculovirus transfection method, low expression level, lipid analyses, overview
gene DGAT1A, recombinant expression of isozyme VgDGAT1A in Saccharomyces cerevisiae strain INVSc1 microsomes, and in Spodoptera frugiperda Sf9 insect cells via recombinant baculovirus transfection method, lipid analyses, overview
gene DGAT1B, recombinant expression of isozyme GmDGAT1B in Spodoptera frugiperda Sf9 insect cells via recombinant baculovirus transfection method, lipid analyses, overview
gene DGAT1B, recombinant expression of isozyme VgDGAT1B in Saccharomyces cerevisiae strain INVSc1 microsomes, and in Spodoptera frugiperda Sf9 insect cells via recombinant baculovirus transfection method, lipid analyses, overview
gene DGAT2, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and phylogenetic analysis, recombinant overexpression of FLAG-tagged enzyme DGAT2 in Nannochloropsis oceanica cells, real-time quantitative PCR expression analysis
gene DGAT2, DNA and amino acid sequence determination and analysis, recombinant expression of NeoDGAT2 cDNA under the control of HSP70-RBCS2 (AR) promoter or beta2-tubulin (beta2-Tub) promoter, both of Chlamydomonas reinhardtii from plasmids pAR-DGAT2 and pB2-DGAT2 in Neochloris oleoabundans cells
gene DGAT2, expression of FLAG-tagged wild-type and mutant enzyme in COS-7 cells
-
gene DGAT2, identified from a transcriptome database, DNA and amino acid sequence determination and analysis of DGAT2 isozymes, DGAT2A, DGAT2B, DGAT2D, and DGAT2E, sequence comparisons and phylogenetic analysis, recombinant expression in TAG-deficient Saccharomyces cerevisiae strain H1246. Triacylglycerol (TAG) is synthesized with expression of HpDGAT2A and HPDGAT2D, but no TAG is detected in cells expressing HpDGAT2B and HpDGAT2E
gene DGAT2, localization of DGAT2 at 11q13.5, DNA and amino acid sequence determination and analysis, expression analysis, expression in Saccharomyces cerevisiae
gene DGAT2.1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis of DGAT isozymes, recombinant expression in the TAG-deficient Saccharomyces cerevisiae strain H1246, LiDGATs of type 1 and 2 do not co-localize with each other at least in yeast
gene DGAT2B, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, subcloning in Escherichia coli strain DH5alpha, recombinant expression of wild-type (MaDGAT2) Mortierella alpina gene in and complementation of Saccharomyces cerevisiae strain H1246 defective in synthesis of neutral lipid, lipid analysis of the lipid content of the recombinant culture harboring MaDGAT2 gene
A0A2D2CI94, KY859195
gene DGAT2D, functional recombinant expression in Saccharomyces cerevisiae mutant strain H1246 and in Saccharomyces cerevisiae wild-type strain YPH499a, isozyme GmDGAT2D complements the TAG deficiency of mutant strain H1246. Recombinant ectopic GmDGAT2D expression in soybean hairy roots, recombinant expression in Arabidopsis thaliana using the Agrobacterium tumefaciens GV3101 transfection method. Quantitative RT-PCR enzyme expression analysis. Determination of the subcellular localization of GmDGAT-GFP performed by using tobacco leaf infiltration
gene DGAT3, Arabidopsis thaliana contains a single DGAT3 gene, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic tree
gene DGAT3, Glycine max contains 2 DGAT3 genes, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic tree
gene DGAT3, heterologous expression in Saccharomyces cerevisiae TAG-deficient mutant strain H1246
-
gene DGTT1, quantitative real-time PCR enzyme expression analysis, recombinant expression of the isozyme in TAG-deficient Saccharomyces cerevisiae strain H1246 microsomes, expression of CrDGTT1 restores TAG biosynthesis
gene DGTT2, quantitative real-time PCR enzyme expression analysis, recombinant expression of the isozyme in TAG-deficient Saccharomyces cerevisiae strain H1246 microsomes, expression of CrDGTT2 restores TAG biosynthesis
gene DGTT3, quantitative real-time PCR enzyme expression analysis, recombinant expression of the isozyme in TAG-deficient Saccharomyces cerevisiae strain H1246 microsomes, expression of CrDGTT3 restores TAG biosynthesis
gene MaDGAT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression in Saccharomyces cerevisiae strain H1246
gene mMaDGAT2B, subcloning in Escherichia coli strain DH5alpha, synthetic gene after codon-optimization, recombinant expression of codon-optimized (mMaDGAT2) Mortierella alpina gene in and complementation of Saccharomyces cerevisiae strain H1246 defective in synthesis of neutral lipid, lipid analysis of the lipid content of the recombinant culture harboring mMaDGAT2 gene
A0A2D2CI94, KY859195
gene Rv3371, specific to pathogenic mycobacteria, leads to modifications in colony morphotype, bacterial architecture, cell surface properties, and to elevated triacylglycerol (TAG) levels when expressed in Mycolicibacterium smegmatis (Mycobacterium smegmatis)
gene SiDGAT1, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, quantitative real-time PCR expression analysis, functional recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, higher oil content in SiDGAT gene-transformed mutants, lipid analysis, overview
M1E7W9, XP_011098009
gene SiDGAT2, DNA and amino acid sequence determination and analysis, genetic structure, phylogenetic analysis, quantitative real-time PCR expression analysis, functional recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 and complementation, higher oil content in SiDGAT gene-transformed mutants, lipid analysis, overview
M1E7W9, XP_011098009
gene Tcur_3818, DNA and amino acid sequence determination and analysis, recombinant expression of DGAT in Escherichia coli triggering rapid triglyceride accumulation in Escherichia coli. tDGAT is associated with the lipids accumulated in recombinant Escherichia coli cells
gene tgs1, expressionin Escherichia coli, the recombinant enzyme prefers C26 : 0-CoA for triglycerol accumulation
-
gene WSD, Glycine max contains a single WSD gene, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic tree
gene WSD1, Arabidopsis thaliana contains 11 WS/DGAT genes, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic tree
gene WSD4, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast
gene WSD5, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast
gene YALI0_E32769g, sequence comparisons, recombinant expression of MBP-tagged wild-type enzyme and mutants in Escherichia coli strain BL21(DE3) pLysS
gene YlDGA1, overexpression in DGAT-deleted Yarrowia lipolytica strain Q4 (JMY1877), subcloning in Escherichia coli
gene YlDGA2, overexpression in DGAT-deleted Yarrowia lipolytica strain Q4 (JMY1877), subcloning in Escherichia coli
genes CeDGAT1, CeDGAT2a, and CeDGAT2b, functional overexpression of the isozymes in yeast mutant strain H1246, and in Arabidopsis thaliana seeds of TAG1 mutant and wild-type lines via Agrobacterium tumefaciens strain EHA105 transfection method, quantitative RT-PCR enzyme expression analysis
-
isoform DGAT2 is expressed in Saccharomyces cerevisiae mutant strain H1246
-
isozyme BnaC.DGAT1.a, recombinant expression of wild-type and truncated mutant enzymes in Saccharomyces cerevisiae strain H1246, which is devoid of TAG biosynthesis
MrDGAT2A and MrDGAT2B
-
mutants AS11 and ABX45, ABX45 germination is delayed
-
phylogenetic analysis, quantitative RT-PCR expression analysis, recombinant expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain delta4
quantitative PCR expression analysis of PtDGATX, comparison of isozyme transcription levels and dynamics
quantitative PCR expression analysis of PtDGATX, comparison of isozyme transcription levels and dynamics, gene expression analysis, functional recombinant expression of His6-tagged DGATX using promoters of fcpA and fcpB genes (and fcpA terminators) in Saccharomyces cerevisiae strain H1246 that is deficient in TAG biosynthesis, a substantial amount of wax ester is detected in H1246-PtDGAT, indicating the dual function of PtDGATX. Endogenous overexpression of PtWS/DGAT. In H1246-PtWS/DGAT, TAG and wax ester account for 10.51 and 5.17%, respectively, of the total lipids. C16:1 and C18:1 are the dominant fatty acid species in total lipids, fatty acid content of total lipid in Saccharomyces cerevisiae, overview
randomly mutagenized libraries expressed in Saccharomyces cerevisiae
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recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 in microsomes
recombinant expression of CzDGAT2 isozymes in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 in microsomes
recombinant expression of FLAG- or myc-tagged DGAT-2 fused to biotin ligase in HEK-293T cells and in COS-7 cells
RtDGATa, sequence comparisons and phylogenetic analysis, recombinant expression of isozyme RtDGATa in Saccharomyces cerevisiae TAG-deficient quadruple mutant strain H1246, subcloning in Escherichia coli Top10 cells. The expression of RtDGATa does not alter the TAG content in Saccharomyces cerevisiae strain H1246
RtDGATb, sequence comparisons and phylogenetic analysis, recombinant expression of isozyme RtDGATb in Saccharomyces cerevisiae TAG-deficient quadruple mutant strain H1246, subcloning in Escherichia coli Top10 cells. The expression of RtDGATb completely resumes TAG biosynthesis in Saccharomyces cerevisiae strain H1246
short-term hepatic overexpression of DGAT1 in mice using adenoviral vectors results in 2.0fold increases in the triglyceride content of liver, the increase in hepatic triglyceride content has no effect on the production rate of VLDL triglyceride or apoB
short-term hepatic overexpression of DGAT2 in mice using adenoviral vectors results in 2.4fold increases in the triglyceride content of liver, the increase in hepatic triglyceride content has no effect on the production rate of VLDL triglyceride or apoB
the enzyme without the N-terminal 29 amino acids is expressed in Saccharomyces cerevisiae mutant DELTAdga1
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wild-type and N-terminal fragment
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