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Literature summary for 2.3.1.20 extracted from

  • Stone, S.J.; Levin, M.C.; Farese, R.V.
    Membrane topology and identification of key functional amino acid residues of murine acyl-CoA:diacylglycerol acyltransferase-2 (2006), J. Biol. Chem., 281, 40273-40282.
    View publication on PubMed
Search for more literature for 2.3.1.20

Cloned(Commentary)

Cloned (Comment) Organism
gene DGAT2, expression of FLAG-tagged wild-type and mutant enzyme in COS-7 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
C87S site-directed mutagenesis, the mutant shows unaltered activity compared to the wild-type enzyme Mus musculus
H161A site-directed mutagenesis, the mutant shows about 50% reduced activity compared to the wild-type enzyme Mus musculus
H161A/P162G/H163A site-directed mutagenesis, the mutant shows over 80% reduced activity compared to the wild-type enzyme Mus musculus
H163A site-directed mutagenesis, the mutant shows over 80% reduced activity compared to the wild-type enzyme Mus musculus
P162G site-directed mutagenesis, the mutant shows about 40% reduced activity compared to the wild-type enzyme Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane DGAT2 is an integral membrane protein with both the N and C terminus oriented toward the cytosol, the loop between the two transmembrane domains of DGAT2is not exposed to the cytosol Mus musculus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-1,2-diacylglycerol + acyl-CoA Mus musculus the final step in triacylglycerol synthesis is catalyzed by the acyl-CoA:diacylglycerol acyltransferase enzymes, DGAT1 and DGAT2 triacylglycerol + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
acyl-CoA + 1,2-diacyl-sn-glycerol = CoA + triacylglycerol the conserved sequence 161HPHG164 is important for activity Mus musculus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-1,2-diacylglycerol + acyl-CoA the final step in triacylglycerol synthesis is catalyzed by the acyl-CoA:diacylglycerol acyltransferase enzymes, DGAT1 and DGAT2 Mus musculus triacylglycerol + CoA
-
 ?
sn-1,2-diacylglycerol + acyl-CoA the enzyme contains a neutral lipid binding sequence 80FLVLGVAC87 residing in the first transmembrane domain Mus musculus triacylglycerol + CoA
-
 ?

Synonyms

Synonyms Comment Organism
acyl-CoA:diacylglycerol acyltransferase-2
-
 Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus