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Literature summary for 2.3.1.20 extracted from
- Cytosolic triacylglycerol biosynthetic pathway in oilseeds. Molecular cloning and expression of peanut cytosolic diacylglycerol acyltransferase (2006), Plant Physiol., 141, 1533-1543.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as His-tagged enzyme in Escherichia coli strain BL21 (DE3), phylogenetic analysis | Arachis hypogaea |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Arachis hypogaea | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Arachis hypogaea |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
x * 41000, recombinant His-tagged enzyme, SDS-PAGE | Arachis hypogaea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-diacylglycerol + acyl-CoA | Arachis hypogaea | the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols | triacylglycerol + CoA | - |
? | |
| 1,2-diacylglycerol + acyl-CoA | Arachis hypogaea JL 24 | the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols | triacylglycerol + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arachis hypogaea | - |
- |
- |
| Arachis hypogaea | Q2KP14 | - |
- |
| Arachis hypogaea JL 24 | Q2KP14 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 1724fold from developing cotyldons by hydrophobic interaction and adsorption chromatography, followed by gel filtration and heparin affinity chromatography to homogeneity | Arachis hypogaea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | developing | Arachis hypogaea | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificity of native and recombinant enzyme | Arachis hypogaea |
| 0.0071 | - |
purified native enzyme | Arachis hypogaea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-diacylglycerol + acyl-CoA | the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols | Arachis hypogaea | triacylglycerol + CoA | - |
? | |
| 1,2-diacylglycerol + acyl-CoA | the enzyme catalyzes the final step in triacylglycerol biosynthesis that acylates diacylglycerol to triacylglycerols | Arachis hypogaea JL 24 | triacylglycerol + CoA | - |
? | |
| 1,2-dipalmitoyl-sn-glycerol + oleoyl-CoA | - |
Arachis hypogaea | 1,2-dipalmitoyl-3-oleoyl-sn-glycerol + CoA | - |
? | |
| 1,2-dipalmitoyl-sn-glycerol + palmitoyl-CoA | - |
Arachis hypogaea | tripalmitin + CoA | - |
? | |
| 1,2-dipalmitoyl-sn-glycerol + stearoyl-CoA | - |
Arachis hypogaea | 1,2-dipalmitoyl-3-stearoyl-sn-glycerol + CoA | - |
? | |
| additional information | substrate specificity of native and recombinant enzyme, the native and the recombinant enzyme do not show wax ester synthase activity in contrast to other DGATs, no activity with hexadecanol, glycerol-3-phosphate, monoacylglycerol, lysophosphatidic acid, and lysophosphatidylcholine, oleoyl-CoA is the preferred acyl donor as compared to palmitoyl- and stearoyl-CoAs | Arachis hypogaea | ? | - |
? | |
| additional information | substrate specificity of native and recombinant enzyme, the native and the recombinant enzyme do not show wax ester synthase activity in contrast to other DGATs, no activity with hexadecanol, glycerol-3-phosphate, monoacylglycerol, lysophosphatidic acid, and lysophosphatidylcholine, oleoyl-CoA is the preferred acyl donor as compared to palmitoyl- and stearoyl-CoAs | Arachis hypogaea JL 24 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41000, recombinant His-tagged enzyme, SDS-PAGE | Arachis hypogaea |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acyl-CoA:diacylglycerol acyltransferase | - |
Arachis hypogaea |
| DGAT | - |
Arachis hypogaea |
| diacylglycerol acyltransferase | - |
Arachis hypogaea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arachis hypogaea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Arachis hypogaea |
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Release 2023.1 (January 2023)
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