Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Brassica napus | |
bovine serum albumin | 5fold at 3-4 mg/ml | Brassica napus | |
CoA | - |
Brassica napus | |
additional information | effectivity of detergents used in in vitro assays, overview | Spinacia oleracea | |
additional information | effectivity of detergents used in in vitro assays, overview | Zea mays | |
additional information | effectivity of detergents used in in vitro assays, overview | Glycine max | |
additional information | effectivity of detergents used in in vitro assays, overview | Umbelopsis ramanniana | |
additional information | effectivity of detergents used in in vitro assays, overview | Ricinus communis | |
additional information | effectivity of detergents used in in vitro assays, overview | Mus musculus | |
additional information | effectivity of detergents used in in vitro assays, overview | Brassica napus | |
additional information | effectivity of detergents used in in vitro assays, overview | Cuphea sp. | |
additional information | effectivity of detergents used in in vitro assays, overview | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
DGAT1, a single copy gene, DNA and amino acid sequence determination and analysis, phylogenetic tree, functional overexpression in tobacco plants | Arabidopsis thaliana |
DGAT1, DNA and amino acid sequence determination and analysis | Arachis hypogaea |
DGAT1, DNA and amino acid sequence determination and analysis | Olea europaea |
DGAT1, DNA and amino acid sequence determination and analysis, phylogenetic tree | Ricinus communis |
DGAT1, DNA and amino acid sequence determination and analysis, phylogenetic tree | Brassica napus |
DGAT1, DNA and amino acid sequence determination and analysis, phylogenetic tree | Nicotiana tabacum |
DGAT2, DNA and amino acid sequence determination and analysis, DGAT2 is a truncated enzyme version, phylogenetic tree | Brassica napus |
DNA and amino acid sequence determination and analysis | Acinetobacter calcoaceticus |
DNA and amino acid sequence determination and analysis, phylogenetic tree | Carthamus tinctorius |
DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in insect cells | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a recombinant acyl-CoA binding protein increased the activity of Arabidopsis thaliana DGAT1 expressed in insect cell culture by up to 70%, overexpression of AtDGAT1 cDNA from Arabidopsis does not complement the knocked-out acyl-CoA:cholesterol acyltransferases, ACAT, EC 2.3.1.26, in a yeast double-mutant | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Bromooctanoate | - |
Brassica napus | |
bovine serum albumin | strong inhibition | Carthamus tinctorius | |
Ca2+ | - |
Carthamus tinctorius | |
Hg2+ | - |
Carthamus tinctorius | |
Iodide | - |
Brassica napus | |
Mg2+ | - |
Carthamus tinctorius | |
niacin | - |
Arabidopsis thaliana | |
Soybean trypsin inhibitor | - |
Brassica napus | |
tannin | - |
Brassica napus | |
Zn2+ | - |
Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | thylakoid and envelope membranes, not in the stroma | Arabidopsis thaliana | 9507 | - |
cytosol | - |
Arachis hypogaea | 5829 | - |
endoplasmic reticulum | - |
Spinacia oleracea | 5783 | - |
endoplasmic reticulum | - |
Glycine max | 5783 | - |
endoplasmic reticulum | - |
Umbelopsis ramanniana | 5783 | - |
endoplasmic reticulum | - |
Ricinus communis | 5783 | - |
endoplasmic reticulum | - |
Mus musculus | 5783 | - |
endoplasmic reticulum | - |
Brassica napus | 5783 | - |
endoplasmic reticulum | - |
Cuphea sp. | 5783 | - |
membrane | - |
Rattus norvegicus | 16020 | - |
membrane | - |
Zea mays | 16020 | - |
membrane | - |
Acinetobacter calcoaceticus | 16020 | - |
membrane | - |
Carthamus tinctorius | 16020 | - |
membrane | - |
Nicotiana tabacum | 16020 | - |
microsome | - |
Rattus norvegicus | - |
- |
microsome | - |
Carthamus tinctorius | - |
- |
microsome | - |
Ricinus communis | - |
- |
microsome | - |
Brassica napus | - |
- |
microsome | - |
Cuphea sp. | - |
- |
oil body | - |
Carthamus tinctorius | - |
- |
oil body | - |
Umbelopsis ramanniana | - |
- |
rough endoplasmic reticulum | - |
Zea mays | 5791 | - |
thylakoid membrane | - |
Arabidopsis thaliana | 42651 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus | |
Mg2+ | - |
Zea mays | |
Mg2+ | - |
Carthamus tinctorius | |
Mg2+ | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-diacylglycerol + acyl-CoA | Rattus norvegicus | - |
triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Spinacia oleracea | - |
triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Zea mays | - |
triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Glycine max | - |
triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Acinetobacter calcoaceticus | - |
triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Arachis hypogaea | - |
triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Carthamus tinctorius | - |
triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Umbelopsis ramanniana | - |
triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Olea europaea | - |
triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Ricinus communis | - |
triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Mus musculus | - |
triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Cuphea sp. | - |
triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Nicotiana tabacum | - |
triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Brassica napus | DGAT catalyzes the transfer of an acyl moiety between two DAG molecules to form triacylglycerol and monoacylglycerol | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | Arabidopsis thaliana | role in leaf metabolism, overview | triacylglycerol + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Arabidopsis thaliana | - |
- |
- |
Arabidopsis thaliana | Q9SLD2 | - |
- |
Arachis hypogaea | - |
- |
- |
Brassica napus | - |
- |
- |
Brassica napus | Q9XGR5 | DGAT1 | - |
Brassica napus | Q9XGV4 | DGAT2 | - |
Carthamus tinctorius | - |
- |
- |
Cuphea sp. | - |
- |
- |
Glycine max | - |
- |
- |
Mus musculus | - |
- |
- |
Mus musculus | Q9Z2A7 | DGAT1 | - |
Nicotiana tabacum | Q9SEG9 | - |
- |
Olea europaea | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Ricinus communis | - |
- |
- |
Ricinus communis | Q67C39 | - |
- |
Spinacia oleracea | - |
- |
- |
Umbelopsis ramanniana | - |
- |
- |
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme from cotyledons to near homogeneity | Glycine max |
native enzyme from the oil body fraction to homogeneity | Umbelopsis ramanniana |
native intestinal enzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cotyledon | - |
Glycine max | - |
endosperm | - |
Ricinus communis | - |
fruit | - |
Olea europaea | - |
intestine | - |
Rattus norvegicus | - |
leaf | - |
Spinacia oleracea | - |
leaf | - |
Arabidopsis thaliana | - |
leaf | - |
Nicotiana tabacum | - |
liver | - |
Rattus norvegicus | - |
seed | - |
Ricinus communis | - |
seed | - |
Cuphea sp. | - |
seed | developing | Glycine max | - |
seed | maturing | Carthamus tinctorius | - |
seed | developing oilseed | Brassica napus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-diacylglycerol + acyl-CoA | - |
Rattus norvegicus | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Spinacia oleracea | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Zea mays | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Glycine max | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Acinetobacter calcoaceticus | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Arachis hypogaea | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Carthamus tinctorius | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Umbelopsis ramanniana | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Olea europaea | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Ricinus communis | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Mus musculus | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Brassica napus | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Cuphea sp. | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Arabidopsis thaliana | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | - |
Nicotiana tabacum | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | DGAT catalyzes the transfer of an acyl moiety between two DAG molecules to form triacylglycerol and monoacylglycerol | Brassica napus | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | role in leaf metabolism, overview | Arabidopsis thaliana | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | acyl-CoA specificity, no acylation activity with phosphoatidylcholine or oleic acid, overview | Umbelopsis ramanniana | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | acyl-CoA specificity, overview | Spinacia oleracea | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | acyl-CoA specificity, overview | Zea mays | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | acyl-CoA specificity, overview | Glycine max | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | acyl-CoA specificity, overview | Olea europaea | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | acyl-CoA specificity, overview | Mus musculus | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | microsomal DGAT from castor bean exhibited high activity toward diacylglycerols containing unusual fatty acids, e.g. ricinoleic acids, at both sn-1 and sn-2 positions, acyl-CoA specificity, overview | Ricinus communis | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + acyl-CoA | microsomal DGAT from Cuphea exhibits high activity toward diacylglycerols containing unusual fatty acids, e.g. lauric acids, at both sn-1 and sn-2 positions, acyl-CoA specificity, overview | Cuphea sp. | triacylglycerol + CoA | - |
? | |
1,2-diacylglycerol + erucoyl-CoA | - |
Brassica napus | 1,2-diacyl-3-erucoylglycerol + CoA | - |
? | |
1,2-diacylglycerol + oleoyl-CoA | - |
Brassica napus | 1,2-diacyl-3-oleoylglycerol + CoA | - |
? | |
1,2-diacylglycerol + palmitoyl-CoA | - |
Brassica napus | 1,2-diacyl-3-palmitoylglycerol + CoA | - |
? | |
1,2-diacylglycerol + stearoyl-CoA | - |
Brassica napus | 1,2-diacyl-3-stearoylglycerol + CoA | - |
? | |
1-palmitoyl-2-oleoyl glycerol + acyl-CoA | - |
Mus musculus | 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA | - |
? | |
1-palmitoyl-2-oleoyl glycerol + acyl-CoA | - |
Rattus norvegicus | 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA | - |
? | |
1-palmitoyl-2-oleoyl glycerol + acyl-CoA | - |
Spinacia oleracea | 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA | - |
? | |
1-palmitoyl-2-oleoyl glycerol + acyl-CoA | - |
Zea mays | 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA | - |
? | |
1-palmitoyl-2-oleoyl glycerol + acyl-CoA | - |
Glycine max | 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA | - |
? | |
1-palmitoyl-2-oleoyl glycerol + acyl-CoA | - |
Arabidopsis thaliana | 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA | - |
? | |
1-palmitoyl-2-oleoyl glycerol + acyl-CoA | - |
Ricinus communis | 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA | - |
? | |
1-palmitoyl-2-oleoyl glycerol + acyl-CoA | - |
Carthamus tinctorius | 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA | - |
? | |
1-palmitoyl-2-oleoyl glycerol + acyl-CoA | - |
Umbelopsis ramanniana | 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA | - |
? | |
1-palmitoyl-2-oleoyl glycerol + acyl-CoA | - |
Olea europaea | 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA | - |
? | |
1-palmitoyl-2-oleoyl glycerol + acyl-CoA | - |
Cuphea sp. | 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA | - |
? | |
1-palmitoyl-2-oleoylglycerol + acyl-CoA | - |
Brassica napus | 1-palmitoyl-2-oleoyl-3-acylglycerol + CoA | - |
? | |
2,3-diacylglycerol + acyl-CoA | - |
Rattus norvegicus | triacylglycerol + CoA | - |
? | |
2,3-diacylglycerol + acyl-CoA | - |
Carthamus tinctorius | triacylglycerol + CoA | - |
? | |
diolein + acyl-CoA | - |
Mus musculus | ? | - |
? | |
diolein + acyl-CoA | - |
Rattus norvegicus | ? | - |
? | |
diolein + acyl-CoA | - |
Spinacia oleracea | ? | - |
? | |
diolein + acyl-CoA | - |
Zea mays | ? | - |
? | |
diolein + acyl-CoA | - |
Glycine max | ? | - |
? | |
diolein + acyl-CoA | - |
Arabidopsis thaliana | ? | - |
? | |
diolein + acyl-CoA | - |
Brassica napus | ? | - |
? | |
diolein + acyl-CoA | - |
Ricinus communis | ? | - |
? | |
diolein + acyl-CoA | - |
Carthamus tinctorius | ? | - |
? | |
diolein + acyl-CoA | - |
Umbelopsis ramanniana | ? | - |
? | |
diolein + acyl-CoA | - |
Olea europaea | ? | - |
? | |
diolein + acyl-CoA | - |
Cuphea sp. | ? | - |
? | |
dipalmitin + acyl-CoA | - |
Mus musculus | ? | - |
? | |
dipalmitin + acyl-CoA | - |
Rattus norvegicus | ? | - |
? | |
dipalmitin + acyl-CoA | - |
Spinacia oleracea | ? | - |
? | |
dipalmitin + acyl-CoA | - |
Zea mays | ? | - |
? | |
dipalmitin + acyl-CoA | - |
Glycine max | ? | - |
? | |
dipalmitin + acyl-CoA | - |
Arabidopsis thaliana | ? | - |
? | |
dipalmitin + acyl-CoA | - |
Brassica napus | ? | - |
? | |
dipalmitin + acyl-CoA | - |
Ricinus communis | ? | - |
? | |
dipalmitin + acyl-CoA | - |
Carthamus tinctorius | ? | - |
? | |
dipalmitin + acyl-CoA | - |
Umbelopsis ramanniana | ? | - |
? | |
dipalmitin + acyl-CoA | - |
Olea europaea | ? | - |
? | |
dipalmitin + acyl-CoA | - |
Cuphea sp. | ? | - |
? | |
additional information | acyl-CoA specificity, overview | Brassica napus | ? | - |
? | |
additional information | acyl-CoA specificity, overview | Arabidopsis thaliana | ? | - |
? | |
additional information | DGAT is also catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22 | Umbelopsis ramanniana | ? | - |
? | |
additional information | DGAT is catalyzing the reactions of the monoacylglycerol transferase, EC 2.3.1.22, of the diacylglycerol transferase, EC 2.3.1.20, of the wax synthase, EC 2.3.1.75, and of the acyl-CoA:retinol acyltransferase, EC 2.3.1.76, overview | Mus musculus | ? | - |
? | |
additional information | no activity with sn-1,3-diacylglycerols, acyl-CoA specificity, overview | Rattus norvegicus | ? | - |
? | |
additional information | no activity with sn-1,3-diacylglycerols, water-soluble diacylglycerols, including rac-1,2-diacetylglycerol and rac-1,2-dibutyrylglycerol, are poor substrates of DGAT from safflower seeds, acyl-CoA specificity, overview | Carthamus tinctorius | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | - |
Glycine max |
Synonyms | Comment | Organism |
---|---|---|
DGAT | - |
Rattus norvegicus |
DGAT | - |
Spinacia oleracea |
DGAT | - |
Zea mays |
DGAT | - |
Glycine max |
DGAT | - |
Acinetobacter calcoaceticus |
DGAT | - |
Arachis hypogaea |
DGAT | - |
Carthamus tinctorius |
DGAT | - |
Umbelopsis ramanniana |
DGAT | - |
Olea europaea |
DGAT | - |
Ricinus communis |
DGAT | - |
Mus musculus |
DGAT | - |
Brassica napus |
DGAT | - |
Cuphea sp. |
DGAT | - |
Arabidopsis thaliana |
DGAT | - |
Nicotiana tabacum |
diacylglycerol acyltransferase | - |
Rattus norvegicus |
diacylglycerol acyltransferase | - |
Spinacia oleracea |
diacylglycerol acyltransferase | - |
Zea mays |
diacylglycerol acyltransferase | - |
Glycine max |
diacylglycerol acyltransferase | - |
Acinetobacter calcoaceticus |
diacylglycerol acyltransferase | - |
Arachis hypogaea |
diacylglycerol acyltransferase | - |
Carthamus tinctorius |
diacylglycerol acyltransferase | - |
Umbelopsis ramanniana |
diacylglycerol acyltransferase | - |
Olea europaea |
diacylglycerol acyltransferase | - |
Ricinus communis |
diacylglycerol acyltransferase | - |
Mus musculus |
diacylglycerol acyltransferase | - |
Brassica napus |
diacylglycerol acyltransferase | - |
Cuphea sp. |
diacylglycerol acyltransferase | - |
Arabidopsis thaliana |
diacylglycerol acyltransferase | - |
Nicotiana tabacum |