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2.3.1.20: diacylglycerol O-acyltransferase

This is an abbreviated version!
For detailed information about diacylglycerol O-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.20

Reaction

acyl-CoA
+
1,2-diacyl-sn-glycerol
=
CoA
+
triacylglycerol

Synonyms

1,2-diacylglycerol acyltransferase, 2 diacylglycerol acyltransferase, ACY99349, acyl CoA:1,2-diacylglycerol acyltransferase, acyl CoA:diacylglycerol acyltransferase, acyl CoA:diacylglycerol acyltransferase 1, acyl CoA:diacylglycerol acyltransferase-2, acyl coenzyme A:diacylglycerol acyltransferase, acyl coenzyme A:diacylglycerol acyltransferase 1, acyl coenzyme A:diacylglycerol acyltransferase 2, acyl-CoA-dependent diacylglycerol acyltransferase, acyl-CoA: diacylglycerol acyltransferase, acyl-CoA:1,2-diacylglycerol O-acyltransferase, acyl-CoA:1,2-dioleoyl-sn-glycerol acyltransferase, acyl-CoA:diacylglycerol acyltransferase, acyl-CoA:diacylglycerol acyltransferase 1, acyl-CoA:diacylglycerol acyltransferase 2, acyl-CoA:diacylglycerol acyltransferase-2, acyl-CoA:diacylglycerol O-acyltransferase1, acyl-coenzyme A:diacylglycerol acyltransferase, acyltransferase, diacylglycerol, AtDGAT1A, AtDGAT3, AtfA, AtfG25, bifunctional DGAT/wax ester synthase, bifunctional wax synthase/DGAT, bifunctional WS/DGAT, BjDGAT1, BjDGAT2, BnaC.DGAT1.a, BnaDGAT1, BnaDGAT1.a, BnaDGAT1.b, BstDGAT1, CaDGAT1, CeDGAT1, CeDGAT2a, CeDGAT2b, CoDGAT2, CpuDGAT1, CrDGTT1, CrDGTT2, CrDGTT3, CsDGAT1B, CzDGAT1B, CzDGAT2, CzDGAT2A, CzDGAT2B, CzDGAT2C, CzDGAT2D, CzDGAT2E, CzDGAT2F, CzDGAT2G, DAG acyltransferase, DAGAT, DC3, DGA1, Dga1p, DGA2, DGAT, DGAT 1, DGAT 2, DGAT type 2, DGAT-1, DGAT-2, DGAT1, DGAT1-2, DGAT1-4, DGAT1-type enzyme, DGAT1.a, DGAT1A, DGAT1B, DGAT2, DGAT2-1, DGAT2.1, DGAT2.2, DGAT2.3, DGAT2A, DGAT2b, DGAT3, DGTT, diacylglycerol acyltransferase, diacylglycerol acyltransferase 1, diacylglycerol acyltransferase 2, diacylglycerol acyltransferase 3, diacylglycerol acyltransferase type 1, diacylglycerol acyltransferase type 2, diacylglycerol acyltransferase-1, diacylglycerol acyltransferase-2, diacylglycerol acyltransferase1, diacylglycerol acyltransferase2, diacylglycerol O-acyltransferase 1A, diacylglycerol O-acyltransferase 2, diacylglycerol O-acyltransferase 2D, diacylglycerol O-acyltransferase type-1, diacylglycerol O-acyltransferase-1, diglyceride acyltransferase, diglyceride O-acyltransferase, dual-function diacylglycerol acyltransferase, EC 2.3.1.124, ElDGAT1A, FBPA, Gat1, GmDGAT1A, GmDGAT1B, GmDGAT2D, GmDGAT3, HpDGAT2A, HpDGAT2B, HpDGAT2D, HpDGAT2E, Ma2, MaDGAT, MaDGAT2, MFAT, MGAT, mMaDGAT2, monoacylglycerol acyltransferase, More, MtDGAT1, multifunctional O-acyltransferase, NeoDGAT2, palmitoyl-CoA-sn-1,2-diacylglycerol acyltransferase, PDAT, PtDGATX, PtWS/DGAT, RtDGATa, RtDGATb, Rv3088 (TGS4), Rv3130c (TGS1), Rv3234c (TGS3), Rv3371, Rv3734 (TGS2), SAV7256, SCO0958, seed-specific DGAT1, SiDGAT1, SiDGAT2, TAG synthase, Tcur_3818, tDGAT, TmDGAT1, triacylglycerol:acyl-CoA:diacylglycerol acyltransferase, TrWSD4, TrWSD5, type 1 acyl-CoA: diacylglycerol acyltransferase, type 1 acyl-CoA:diacylglycerol acyltransferase, type 1 DGAT, type 1 diacylglycerol acyltransferase, type 2 acyl-CoA:diacylglycerol acyltransferase, type 2 DGAT, type 2 diacylglycerol acyltransferase, type I diacylglycerol acyltransferase, type II diacylglycerol acyltransferase, type-2 diacylglycerol acyltransferase, unspecific bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, VgDGAT1A, VgDGAT1B, wax ester synthase/acyl CoA:diacylglycerol acyltransferase, wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, wax ester synthase/acyl-CoA diacylglycerol acyltransferase, wax ester synthase/acyl-CoA:diacylglycerol acyltransferase, wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase WSD1, wax ester synthase/triacylglycerol:acylCoA acyltransferase, Wdh3563-1, Wdh3563-2, Wdh3563-3, Wdh3563-4, Wdh3563-7, WS/DGAT, WSD1, WSD4, WSD5, YALI0D07986g, YALI0E32769g

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.20 diacylglycerol O-acyltransferase

Molecular Weight

Molecular Weight on EC 2.3.1.20 - diacylglycerol O-acyltransferase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
13278
-
x * 13278, recombinant poly-His-tagged N-terminal enzyme fragment comprising residues 1-116, sequence calculation
13280
-
N-terminal fragment, calculated DNA sequence
13900
recombinant His-tagged cytosolic domain BnaDGAT11-113, gel filtration
150000
-
gel filtration
1539000
-
delipidated enzyme, free of phospholipids, gel filtration
1843000
-
native enzyme monomer, gel filtration
2000000
-
gel filtration
24500
-
1 * 40800 + 2 * 28700 + 2 * 24500, complete structure of the native enzyme from soybean contains ten molecules of the pentameric enzyme, SDS-PAGE, amino acid analysis
25275
HpDGAT2A isozyme, sequence calculation
28700
-
1 * 40800 + 2 * 28700 + 2 * 24500, complete structure of the native enzyme from soybean contains ten molecules of the pentameric enzyme, SDS-PAGE, amino acid analysis
34864
HpDGAT2E isozyme, sequence calculation
36000 - 36500
-
SDS-PAGE
37606
HpDGAT2D isozyme, sequence calculation
38100
2 * 38100, gel filtration
38950
-
x * 38950, calculated from amino acid sequence
39500
-
DGAT2, calculated from amino acid sequence
39600
-
DGAT2A, calculated from amino acid sequence
39912
HpDGAT2B isozyme, sequence calculation
40600
-
DGAT1A, calculated from amino acid sequence
40800
-
1 * 40800 + 2 * 28700 + 2 * 24500, complete structure of the native enzyme from soybean contains ten molecules of the pentameric enzyme, SDS-PAGE, amino acid analysis
41000
x * 41000, recombinant His-tagged enzyme, SDS-PAGE
42000
-
2 * 42000, SDS-PAGE
44500
SDS-PAGE
45000
47000
47100
deduced from cDNA
47400
48900
-
x * 48900 (437 amino-acid residues), calculated
49600
-
Wdh3563-2 deduced from cDNA
49900
-
Wdh3563-4 deduced from cDNA
50000
50200
-
Wdh3563-1 deduced from cDNA
51600
-
Wdh3563-7 deduced from cDNA
51800
x * 51800, deduced from gene sequence
51900
-
Wdh3563-3 deduced from cDNA
53000
-
SDS-PAGE
54800
-
x * 54800, calculated
56800
x * 56800, isoform DGAT1b, calculated from amino acid sequence
56900
-
DGAT1, calculated from amino acid sequence
57100
x * 57100, isoform DGAT1a, calculated from amino acid sequence
58000
2 * 58000, SDS-PAGE
59600
x * 59600, calculated
59900
x * 59900, deduced from gene sequence, x * 60000, SDS-PAGE
60000
60200
x * 60200, calculated
61500
x * 61500, calculated from amino acid sequence
63000
x * 63000, calculated from amino acid sequence
64000
2 * 64000, calculated from amino acid sequence
64500
8600
recombinant His-tagged cytosolic domain BnaDGAT11-80, gel filtration
90000
-
gel filtration, the enzyme is part of large 650000 Da protein complex
94100
gel filtration
97000
gel filtration
additional information