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Literature summary for 2.3.1.20 extracted from

  • Stoveken, T.; Kalscheuer, R.; Malkus, U.; Reichelt, R.; Steinbuchel, A.
    The wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1: characterization of a novel type of acyltransferase (2005), J. Bacteriol., 187, 1369-1376.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ammonium sulfate 45 mM, 10% increase in activity Acinetobacter baylyi ADP1

General Stability

General Stability Organism
bovine serum albumin stabilizes, decrease in activity with buffers such as bis-Tris, HEPES, morpholinoethanesulfonic acid Acinetobacter baylyi ADP1

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 5 mM, 30% inhibition Acinetobacter baylyi ADP1
CoA above 50 mM, about 70% inhibition above 300 mM Acinetobacter baylyi ADP1
MgCl2 5 mM, 17% inhibition Acinetobacter baylyi ADP1
MnCl2 5 mM, 30% inhibition Acinetobacter baylyi ADP1

Localization

Localization Comment Organism GeneOntology No. Textmining
lipid particle
-
Acinetobacter baylyi ADP1
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38100
-
2 * 38100, gel filtration Acinetobacter baylyi ADP1
94100
-
gel filtration Acinetobacter baylyi ADP1

Organism

Organism UniProt Comment Textmining
Acinetobacter baylyi ADP1 Q8GGG1 expression in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Acinetobacter baylyi ADP1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + 1-decanol at 48% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
?
acyl-CoA + 2-cyclohexylethanol at 45% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
?
acyl-CoA + 2-decanol at 39% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
?
acyl-CoA + 4-decanol at 15.6% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
?
acyl-CoA + cyclododecanol at 80% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
?
acyl-CoA + cyclohexandiol at 4.1% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
?
acyl-CoA + cyclohexanol at 32% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
?
acyl-CoA + cyclohexanone oxime at 5.2% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
?
acyl-CoA + hexadecanol
-
Acinetobacter baylyi ADP1 ? + CoA
-
?
acyl-CoA + phenol at 4.1% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
?
acyl-CoA + phenylethanol at 99% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
?
additional information enzyme also has acyl-CoA-monoacylglycerol acyltransferase activity, sn-1 and sn-3 positions are accepted with higher specificity than sn-2 position Acinetobacter baylyi ADP1 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 38100, gel filtration Acinetobacter baylyi ADP1

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 45
-
Acinetobacter baylyi ADP1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 40
-
Acinetobacter baylyi ADP1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
phosphate buffer Acinetobacter baylyi ADP1