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EC 2.3.1.230 Details
EC number
2.3.1.230
Accepted name
2-heptyl-4(1H)-quinolone synthase
Reaction
octanoyl-CoA + (2-aminobenzoyl)acetate = 2-heptyl-4-quinolone + CoA + CO2 + H2O (overall reaction);;(1a) octanoyl-CoA + L-cysteinyl-[PqsC protein] = S-octanoyl-L-cysteinyl-[PqsC protein] + CoA;;(1b) S-octanoyl-L-cysteinyl-[PqsC protein] + (2-aminobenzoyl)acetate = 1-(2-aminophenyl)decane-1,3-dione + CO2 + L-cysteinyl-[PqsC protein];;(1c) 1-(2-aminophenyl)decane-1,3-dione = 2-heptyl-4-quinolone + H2O
Other name(s)
pqsBC (gene names), malonyl-CoA:anthraniloyl-CoA C-acetyltransferase (decarboxylating)
Systematic name
octanoyl-CoA:(2-aminobenzoyl)acetate octanoyltransferase
Comment
The enzyme, characterized from the bacterium Pseudomonas aeruginosa, is a heterodimeric complex. The PqsC subunit acquires an octanoyl group from octanoyl-CoA and attaches it to an internal cysteine residue. Together with the PqsB subunit, the proteins catalyse the coupling of the octanoyl group with (2-aminobenzoyl)acetate, leading to decarboxylation and dehydration events that result in closure of the quinoline ring.
History
created 2013, modified 2017
EC Tree
2.3.1.55 created 1976, deleted 1999
2.3.1.120 created 1990, deleted 1992
2.3.1.124 created 1990, deleted 1992