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4.2.2.3: mannuronate-specific alginate lyase

This is an abbreviated version!
For detailed information about mannuronate-specific alginate lyase, go to the full flat file.

Word Map on EC 4.2.2.3

Reaction

R2-beta-D-mannuronic acid-R1
=
R2-OH
 +
4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-R1

Synonyms

(poly alpha-l-guluronate) lyase, A1 alginate lyase, A1-II, A1-III, A1-IV', A1m, A1mU, A9mC, A9mL, A9mT, AAlyase, AkAly30, AL2, alg, ALG-5, Alg-A, Alg17C, Alg2A, Alg7D, AlgA, AlgB, AlgE7, AlgI, alginase I, alginate lyase, alginate lyase A, alginate lyase A1-II, alginate lyase A1-II', alginate lyase A1-III, alginate lyase AlyPEEC, alginate lyase Atu3025, alginate lyase B, alginate lyase C, alginate lyase I, alginate lyase VI, alginate lyase1-III, AlgL, ALY, ALY-1, Aly-SJ02, Aly1, Aly2, Aly28, Aly30, Aly32, Aly33, Aly35, Aly7B, AlyA, AlyA1, AlyA2, AlyA3, AlyA5, AlyB, AlyDW11, ALYIII, alyPEEC, AlyPI, AlyPM, AlyQ, AlyV5, AlyVI, Atu3025, AXE80_11190, CL2, EC 4.2.99.4, endo-type alginate lyase, endolytic poly(M) lyase, endolytic polymannuronate lyase, exotype alginate lyase, HdAlex, hdalex-1, HdAly, KJ-2 alginate lyase, LbAly28, lyase AlyA, lyase, alginate, Lysis protein, M block-specific polymannuronate lyase, mannuronate alginate lyase, MJ-3 alginate lyase, More, MY04_2544, NIS_0185, Oal17A, oligoalginate lyase, PL-5 alginate lyase, PM lyase, poly(1,4-beta-D-mannuronide) lyase, poly(beta-D-1,4-mannuronide) lyase, Poly(beta-D-mannuronate) lyase, poly(M) lyase, poly(M)lyase, Poly(mana) alginate lyase, poly(mana)alginate lyase, polymannuronate lyase, polyMG-specific alginate lyase, protein PA1167, SP2, V12B01_24254, V12B01_24259, WP_053404615

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.3 mannuronate-specific alginate lyase

Cloned

Cloned on EC 4.2.2.3 - mannuronate-specific alginate lyase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AkAly30 DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3)
AlgL is co-expressed in the algD operon
algL is expressed in pET24a (+)/E. coli BL21 (DE3) system
baculoviral expression system
-
DNA and amino acid sequence determination and analysis
-
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) at 37°C and at 15°C. The recombinant alginate lyase expressed with pColdI at 15°C exhibits the highest alginate-degrading activity, method evaluation and optimization
-
DNA and amino acid sequence determination and analysis, lambda phage display, sequence comparisons
DNA and amino acid sequence determination and analysis, phylogenetic analysis
AB489222
DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
DNA sequence determination and analysis of the gene encoding isozyme A1-III, expression of wild-type and mutant H192A in Escherichia coli
-
expressed as a His-tagged fusion protein
-
expressed as a His-tagged fusion protein in Escherichia coli
expressed as secreted proteins with baculoviral expression systems in Sf9 cells
-
expressed in Escherichia coli B834(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli DH5alpha cells
expressed in yeast and Escherichia coli as a His-tagged fusion protein
-
expression in Escherichia coli
expression in Escherichia coli. Various calcium compounds that enhance the expression of the active enzyme. Maximum activity is observed in the clones cultured in broth containing 50-100% artificial seawater, respectively
-
expression in Escheriochia coli
expression in T7 driven pET vector system
expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli
expression of C-terminally His6-tagged A1-III
expression of C-terminally His6-tagged AlgL
expression of native A1-II' and a truncated A1-II' lacking the N-terminal 80 amino acid residues in Escherichia coli
gene alg17C, expression in Escherichia coli strain BL21(DE3)
gene alg7D, overexpression of soluble His-tagged enzyme lacking the signal peptide in Escherichia coli strain BL21(DE3)
-
gene algL, algL is part of the alginate biosynthetic operon, expression of His-tagged enzyme in Escherichia coli strain C41(DE3)
gene algL, construction of a mutant strain SML2, by introductionof a non-polar mutation, with no alginate lyase activity
-
gene algL, DNA and amino acid sequence determination and analysis, cloning and overexpression in Escherichia coli
gene algL, DNA sequence determination and analysis, overexpression in Escherichia coli BL21(DE3), periplasmic enzyme location
gene algL, expression from pNMAlgL28–362 expression vector as His-tagged enzyme in Escherichia coli strain Origami 2 (DE3)
gene alyA, expression in Escherichia coli strain DH5alpha
-
gene alyVI, expression of GST-fusion wild-type and mutant enzymes in Escherichia coli strain BL-21 LysS
-
gene cl2, DNA and amino acid sequence determination and analysis, expression in Escherichia coli as His-tagged protein
gene encoding the oligoalginate lyase, DNA sequence determination and analysis, genetic mapping
-
gene of alginate lyase (AlyVI) from marine bacterium Vibrio is cloned into the multiple cloning site of the surface display vector pINA1317-YlCWP110 and expressed in cells of Yarrowia lipolytica
-
mutant enzymes H202Q and Y256F
-
overexpressed in Escherichia coli
overexpression in Escherichia coli
overexpression of AlgL in Escherichia coli
-
overexpression of isozyme A1-II in Escherichia coli
-
overexpression of isozyme A1-III in Bacillus subtilis
-
phylogenetic analysis
-
phylogenetic analysis, recombinant expression of His-tagged AlyA5 in Escherichia coli strain BL21(DE3)
protein PA1167, overexpression in Escherichia coli
-
recombinant expression of N-terminally GST-tagged enzyme in Escherichia coli strains BL21(DE3), C41(DE3) and C43(DE3)
-
sequence comparison of PL-5 alginate lyase, recombinant wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS
subcloning of His-tagged wild-type and mutant enzymes in Escherichia coli strain DH5-alpha, expression in Escherichia coli strain BL21(DE3) Rosetta, the enzymes are secreted from periplasmic space
the 3 isozymes are natively expressed as 1 precursor molecule and are posttranslationally processed to monomeric forms, overexpression of all isozymes A1-I, A1-II, and A1-III in Escherichia coli
-
when the alginate lysase gene (aly) is expressed in Escherichi coli, most of the gene product is organized as aggregated insoluble particles. At least 37% of the total alginate lyase is produced in the soluble fraction when aly is coexpressed with DnaK/DnaJ/GrpE chaperone
-