Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Azotobacter vinelandii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 1 mM ZnCl2 reduces the activity to 7.6% of the control level without Zn2+ added, 0.1 mM Zn2+ reduces the activity to 61% | Azotobacter vinelandii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.46 | - |
alginate | - |
Azotobacter vinelandii | |
0.46 | - |
alginate | alginate rich in mannuronic acid | Azotobacter vinelandii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
NaCl | activity is optimal at 0.35 M | Azotobacter vinelandii | |
Zn2+ | 1 mM ZnCl2, 92% loss of activity | Azotobacter vinelandii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, SDS-PAGE | Azotobacter vinelandii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azotobacter vinelandii | - |
- |
- |
Azotobacter vinelandii | O52195 | alginate lyase precursor; recombinant | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | signal peptide is cleaved off, leaving a mature protein of 39000 Da | Azotobacter vinelandii |
Purification (Comment) | Organism |
---|---|
partial | Azotobacter vinelandii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | alginate rich in mannuronic acids, the enzyme cleaves beta-D-mannuronic acid-beta-D-mannuronic acid bonds and beta-D-mannuronic acid-alpha-L-guluronic acid bonds but not alpha-L-guluronic acid-beta-D-mannuronic acid bonds and alpha-L-guluronic acid-alpha-L-guluronic acid-bonds | Azotobacter vinelandii | oligouronides | - |
? | |
alginate | enzyme cleaves beta-D-mannuronic acid-beta-D-mannuronic acid and beta-D-mannuronic acid-alpha-L-guluronic acid bonds but not alpha-L-guluronic acid-alpha-L-guluronic acid or alpha-L-guluronic acid-beta-D-mannuronic acid bonds | Azotobacter vinelandii | unsaturated algino-oligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, SDS-PAGE | Azotobacter vinelandii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | 8.4 | - |
Azotobacter vinelandii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 8.4 | pH 6.7: about 50% of maximal activity, pH 8.1-8.4: optimum | Azotobacter vinelandii |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Azotobacter vinelandii | isoelectric focusing | - |
5.1 |