Cloned (Comment) | Organism |
---|---|
expression of native A1-II' and a truncated A1-II' lacking the N-terminal 80 amino acid residues in Escherichia coli | Sphingomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | 1 mM, 30% inhibition | Sphingomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
gel filtration | Sphingomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas sp. | Q75WP3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Sphingomonas sp. |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 25000, SDS-PAGE | Sphingomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
alginate lyase A1-II' | - |
Sphingomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Sphingomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | 30°C: about 55% of maximal activity, 50°C: about 70% of maximal activity | Sphingomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
pH 7.5, 5 min, stable | Sphingomonas sp. |
35 | - |
pH 7.5, 5 min, about 10% loss of initial activity | Sphingomonas sp. |
40 | - |
pH 7.5, 5 min, about 35% loss of initial activity | Sphingomonas sp. |
45 | - |
pH 7.5, 5 min, about 50% loss of initial activity | Sphingomonas sp. |
50 | - |
pH 7.5, 5 min, about 65% loss of initial activity | Sphingomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Sphingomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.8 | pH 6.0: about 45% of maximal activity, pH 8.8: about 50% of maximal activity | Sphingomonas sp. |