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4.2.2.3: mannuronate-specific alginate lyase

This is an abbreviated version!
For detailed information about mannuronate-specific alginate lyase, go to the full flat file.

Word Map on EC 4.2.2.3

Reaction

R2-beta-D-mannuronic acid-R1
=
R2-OH
 +
4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-R1

Synonyms

(poly alpha-l-guluronate) lyase, A1 alginate lyase, A1-II, A1-III, A1-IV', A1m, A1mU, A9mC, A9mL, A9mT, AAlyase, AkAly30, AL2, alg, ALG-5, Alg-A, Alg17C, Alg2A, Alg7D, AlgA, AlgB, AlgE7, AlgI, alginase I, alginate lyase, alginate lyase A, alginate lyase A1-II, alginate lyase A1-II', alginate lyase A1-III, alginate lyase AlyPEEC, alginate lyase Atu3025, alginate lyase B, alginate lyase C, alginate lyase I, alginate lyase VI, alginate lyase1-III, AlgL, ALY, ALY-1, Aly-SJ02, Aly1, Aly2, Aly28, Aly30, Aly32, Aly33, Aly35, Aly7B, AlyA, AlyA1, AlyA2, AlyA3, AlyA5, AlyB, AlyDW11, ALYIII, alyPEEC, AlyPI, AlyPM, AlyQ, AlyV5, AlyVI, Atu3025, AXE80_11190, CL2, EC 4.2.99.4, endo-type alginate lyase, endolytic poly(M) lyase, endolytic polymannuronate lyase, exotype alginate lyase, HdAlex, hdalex-1, HdAly, KJ-2 alginate lyase, LbAly28, lyase AlyA, lyase, alginate, Lysis protein, M block-specific polymannuronate lyase, mannuronate alginate lyase, MJ-3 alginate lyase, More, MY04_2544, NIS_0185, Oal17A, oligoalginate lyase, PL-5 alginate lyase, PM lyase, poly(1,4-beta-D-mannuronide) lyase, poly(beta-D-1,4-mannuronide) lyase, Poly(beta-D-mannuronate) lyase, poly(M) lyase, poly(M)lyase, Poly(mana) alginate lyase, poly(mana)alginate lyase, polymannuronate lyase, polyMG-specific alginate lyase, protein PA1167, SP2, V12B01_24254, V12B01_24259, WP_053404615

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.2 Acting on polysaccharides
                4.2.2.3 mannuronate-specific alginate lyase

Purification

Purification on EC 4.2.2.3 - mannuronate-specific alginate lyase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
addition of EDTA to the purification system improves the enzyme activity and the yield
-
ammonium sulfate fractionation, Toyopearl CM-650 M column chromatography, and hydroxyapatite column chromatography
ammonium sulfate precipitation and Ni-NTA column chromatography
-
combination of ammonium sulfate precipitation and diethylaminoethyl-Sephacel column chromatography
-
DEAE-Toyopearl 650M column chromatography, Q Sepharose column chromatography, and Superdex 200 gel filtration
DEAE-Toyopearl column chromatography
enzyme form SP2
-
from the culture medium, 135fold, to homogeneity
-
HiTrap SP column chromatography (isoform AlyA1), HiTrap Q column chromatography (isoform AlyA2 and AlyA3), and butyl Sepharose column chromatography (isoform AlyA2)
-
native alginate lyases, by acetone precipitation, anion exchange chromatography, and gel filtration
-
native and recombinant protein
-
native enzyme 1.75fold by ammonium sulfate fractionation and anion exchange chromatography
AB489222
native extracellular enzyme 23.6fold from cell culture supernatant
-
native extracellular enzyme, 140fold
-
native isozyme AkAly28 72.6fold and isozyme isozyme AkAly33 26fold by ammonium sulfate fractionation and cation exchange chromatography
-
native, secreted aly-SJ02 73.4fold by ultrafiltration, anion exchange chromatography, and gel filtration
-
Ni-Sepharose column chromatography
-
partial
partially purified by ultrafiltration
-
purification by centrifugation, ammonium sulfate fractionation, anion-exchange chromatography and size exclusion chromatography
-
purification leads to a mixture of three monomeric alginate lyases with molecular masses of 60.25, 36, and 23 kDa
-
purification using Ni-NTA column
purified by dual ammonium sulfate precipitation
-
recombinant
recombinant Alg17C from Escherichia coli strain BL21(DE3)
recombinant AlgL
-
recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli by metal affinity chromatography
recombinant C-terminally His6-tagged A1-III by nickel affinity chromatography and dialysis, native enzyme by hydroxyapatite chromatography, ultrafiltration, and gel filtration
recombinant C-terminally His6-tagged AlgL by nickel affinity chromatography and dialysis, native enzyme by hydroxyapatite chromatography, ultrafiltration, and gel filtration
recombinant enzyme
recombinant enzyme from Escherichia coli
recombinant enzyme from Escherichia coli, 10.1fold
recombinant enzyme purified on Ni-NTA sepharose
recombinant GST-fusion wild-type and mutant enzymes from Escherichia coli strain BL-21 LysS by glutathione affinity chromatography
-
recombinant His-tagged AkAly30 33fold from Escherihcia coli strain BL21(DE3) by nickel affinity chromatography, native AkAly30 97.58fold by ammonium sulfate fractionation and cation exchange chromatography
recombinant His-tagged AlgL28-362 protein from Escherichia coli strain Origami 2 (DE3) by nickel affinity chrmatography, ultrafiltration, and gel filtration
recombinant His-tagged enzyme by nickel affinity chromatography from Escherichia coli strain BL21(DE3)
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity
recombinant His-tagged enzyme from Escherichia coli strain C41(DE3) by nickel or cobalt affinity chromatography
recombinant His-tagged enzyme from Escherichia coli, 4.8fold
recombinant His-tagged enzyme lacking the signal peptide from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant isozyme A1-II from Escherichia coli, 52.9fold
-
recombinant isozyme A1-III from Bacillus subtilis
-
recombinant isozymes A1-I 11.2fold, A1-II 25.5fold, and A1-III 4.9fold, from Escherichia coli
-
recombinant protein
recombinant protein PA1167
-
recombinant protein, purified by Ni-NTA Sepharose affinity chromatography
recombinant protein, purified using Ni-NTA Sepharose
recombinant soluble His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) Rosetta culture supernatant by nickel affinity chromatography and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS
SuperQ Toyopearl column chromatography, butyl Toyopearl column chromatography, and ammonium sulfate fractionation
using gel filtraton and anion-exchange chromatography
-
using Ni-NTA chromatography
using Ni-Sepharose affinity chromatography