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Literature summary for 4.2.2.3 extracted from

  • Singh, R.; Gupta, V.; Kumari, P.; Kumar, M.; Reddy, C.; Prasad, K.; Jha, B.
    Purification and partial characterization of an extracellular alginate lyase from Aspergillus oryzae isolated from brown seaweed (2011), J. Appl. Phycol., 23, 755-762.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic analysis Aspergillus oryzae

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 2 mM, 95% residual activity; 5% inhibition at 2 mM Aspergillus oryzae
Cd2+ 2 mM, 25% residual activity; 75% inhibition at 2 mM Aspergillus oryzae
EDTA 2 mM, 15% residual activity; 85% inhibition at 2 mM Aspergillus oryzae
EGTA 77% inhibition at 2 mM Aspergillus oryzae
ethylene glycol tetraacetic acid 2 mM, 23% residual activity Aspergillus oryzae
Fe2+ 2 mM, 30% residual activity; 70% inhibition at 2 mM Aspergillus oryzae
glutathione 2 mM, 23% residual activity; 77% inhibition at 2 mM Aspergillus oryzae
Hg2+ 2 mM, 10% residual activity; 90% inhibition at 2 mM Aspergillus oryzae
Ni2+ 2 mM, 65% residual activity; 35% inhibition at 2 mM Aspergillus oryzae
Sr2+ 2 mM, 70% residual activity; 30% inhibition at 2 mM Aspergillus oryzae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information KM value for sodium alginate is 21.5 mg/ml Aspergillus oryzae

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus oryzae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates 5% at 2 mM Aspergillus oryzae
Cu2+ activates 4% at 2 mM Aspergillus oryzae
additional information no or poor effects by Mn2+ and Zn2+ Aspergillus oryzae
NaCl 2 mM, 110% of initial activity, 100 mM, 193% of initial activity Aspergillus oryzae
NaCl activates 10% at 2 mM,96% at 150 mM Aspergillus oryzae
Zn2+ activates 5% at 2 mM Aspergillus oryzae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000 + x * 50000 Aspergillus oryzae
45000
-
x * 45000 plus x * 50000, SDS-PAGE Aspergillus oryzae
50000
-
x * 45000 + x * 50000 Aspergillus oryzae
50000
-
x * 45000 plus x * 50000, SDS-PAGE Aspergillus oryzae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aspergillus oryzae enzymatic depolymerization of sodium alginate, the enzyme shows specificity for cleaving at the beta-1,4 glycosidic bond between polyM and polyG blocks of sodium alginate ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus oryzae
-
isolated from brown alga Dictyota dichotoma
-
Aspergillus oryzae
-
isolated from brown seaweed
-

Purification (Commentary)

Purification (Comment) Organism
-
Aspergillus oryzae
native extracellular enzyme, 140fold Aspergillus oryzae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
67
-
pH 6.5, 35°C Aspergillus oryzae
67.24
-
purified enzyme, pH 6.5, 35°C Aspergillus oryzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alginate specific for cleaving at the beta-1,4 glycosidic bond between polyM and polyG blocks of sodium alginate, producing homopolymeric blocks of polyM and polyG. Enzyme is inefficient in the degradation of polyM and polyG Aspergillus oryzae ?
-
?
additional information enzymatic depolymerization of sodium alginate, the enzyme shows specificity for cleaving at the beta-1,4 glycosidic bond between polyM and polyG blocks of sodium alginate Aspergillus oryzae ?
-
?

Subunits

Subunits Comment Organism
? x * 45000 + x * 50000 Aspergillus oryzae
? x * 45000 plus x * 50000, SDS-PAGE Aspergillus oryzae

Synonyms

Synonyms Comment Organism
alginate lyase
-
Aspergillus oryzae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Aspergillus oryzae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 50 assay range, 80% of maximal activity at 20°C and 45°C, activity is completely lost at 50°C Aspergillus oryzae
25 40 more than 80% of maximum activity Aspergillus oryzae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Aspergillus oryzae

pH Range

pH Minimum pH Maximum Comment Organism
2 10.5 assay range Aspergillus oryzae