Cloned (Comment) | Organism |
---|---|
phylogenetic analysis | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2 mM, 95% residual activity; 5% inhibition at 2 mM | Aspergillus oryzae | |
Cd2+ | 2 mM, 25% residual activity; 75% inhibition at 2 mM | Aspergillus oryzae | |
EDTA | 2 mM, 15% residual activity; 85% inhibition at 2 mM | Aspergillus oryzae | |
EGTA | 77% inhibition at 2 mM | Aspergillus oryzae | |
ethylene glycol tetraacetic acid | 2 mM, 23% residual activity | Aspergillus oryzae | |
Fe2+ | 2 mM, 30% residual activity; 70% inhibition at 2 mM | Aspergillus oryzae | |
glutathione | 2 mM, 23% residual activity; 77% inhibition at 2 mM | Aspergillus oryzae | |
Hg2+ | 2 mM, 10% residual activity; 90% inhibition at 2 mM | Aspergillus oryzae | |
Ni2+ | 2 mM, 65% residual activity; 35% inhibition at 2 mM | Aspergillus oryzae | |
Sr2+ | 2 mM, 70% residual activity; 30% inhibition at 2 mM | Aspergillus oryzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | KM value for sodium alginate is 21.5 mg/ml | Aspergillus oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus oryzae | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates 5% at 2 mM | Aspergillus oryzae | |
Cu2+ | activates 4% at 2 mM | Aspergillus oryzae | |
additional information | no or poor effects by Mn2+ and Zn2+ | Aspergillus oryzae | |
NaCl | 2 mM, 110% of initial activity, 100 mM, 193% of initial activity | Aspergillus oryzae | |
NaCl | activates 10% at 2 mM,96% at 150 mM | Aspergillus oryzae | |
Zn2+ | activates 5% at 2 mM | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000 + x * 50000 | Aspergillus oryzae |
45000 | - |
x * 45000 plus x * 50000, SDS-PAGE | Aspergillus oryzae |
50000 | - |
x * 45000 + x * 50000 | Aspergillus oryzae |
50000 | - |
x * 45000 plus x * 50000, SDS-PAGE | Aspergillus oryzae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aspergillus oryzae | enzymatic depolymerization of sodium alginate, the enzyme shows specificity for cleaving at the beta-1,4 glycosidic bond between polyM and polyG blocks of sodium alginate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
isolated from brown alga Dictyota dichotoma | - |
Aspergillus oryzae | - |
isolated from brown seaweed | - |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus oryzae |
native extracellular enzyme, 140fold | Aspergillus oryzae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
67 | - |
pH 6.5, 35°C | Aspergillus oryzae |
67.24 | - |
purified enzyme, pH 6.5, 35°C | Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | specific for cleaving at the beta-1,4 glycosidic bond between polyM and polyG blocks of sodium alginate, producing homopolymeric blocks of polyM and polyG. Enzyme is inefficient in the degradation of polyM and polyG | Aspergillus oryzae | ? | - |
? | |
additional information | enzymatic depolymerization of sodium alginate, the enzyme shows specificity for cleaving at the beta-1,4 glycosidic bond between polyM and polyG blocks of sodium alginate | Aspergillus oryzae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000 + x * 50000 | Aspergillus oryzae |
? | x * 45000 plus x * 50000, SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
alginate lyase | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 50 | assay range, 80% of maximal activity at 20°C and 45°C, activity is completely lost at 50°C | Aspergillus oryzae |
25 | 40 | more than 80% of maximum activity | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Aspergillus oryzae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 10.5 | assay range | Aspergillus oryzae |