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Literature summary for 4.2.2.3 extracted from
- Cloning and expression of the algL gene, encoding the Azotobacter chroococcum alginate lyase: purification and characterization of the enzyme (1999), J. Bacteriol., 181, 1409-1414.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by EDTA | Azotobacter chroococcum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene algL, DNA and amino acid sequence determination and analysis, cloning and overexpression in Escherichia coli | Azotobacter chroococcum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by EDTA | Azotobacter chroococcum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | highly activating, activation is synergistic with Mg2+ | Azotobacter chroococcum |  |
| Mg2+ | slightly activating, activation is synergistic with K+ | Azotobacter chroococcum | |
| additional information | not affected by Ca2+, Co2+, Mn2+, and Zn2+ | Azotobacter chroococcum | |
| Na+ | slightly activating | Azotobacter chroococcum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
1 * 43000, SDS-PAGE | Azotobacter chroococcum |
| 46000 | - |
gel filtration | Azotobacter chroococcum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Azotobacter chroococcum | O50660 | strain ATCC 4412, gene algL | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli, 10.1fold | Azotobacter chroococcum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 93.88 | - |
purified enzyme | Azotobacter chroococcum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alginic acid | - |
Azotobacter chroococcum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 43000, SDS-PAGE | Azotobacter chroococcum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alginate lyase | - |
Azotobacter chroococcum |
| AlgL | - |
Azotobacter chroococcum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Azotobacter chroococcum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Azotobacter chroococcum |
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