Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Vibrio splendidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 1 mM, about 20% of initial activity | Vibrio splendidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
alginate | pH 7.5, 25°C | Vibrio splendidus | |
0.036 | - |
alginate | pH 8.5, 25°C | Vibrio splendidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 1.2fold increase in enzymatic activity | Vibrio splendidus | |
Ca2+ | 1 mM, 1.5fold increase in enzymatic activity | Vibrio splendidus | |
Na+ | up to 10fold increase in activity, optimum concentration 1 M | Vibrio splendidus | |
Na+ | up to 3fold increase in activity, optimum concentration 400 mM | Vibrio splendidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
gel filtration | Vibrio splendidus |
68200 | - |
gel filtration | Vibrio splendidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio splendidus | A3UR40 | - |
- |
Vibrio splendidus | A3UR41 | - |
- |
Vibrio splendidus 12B01 | A3UR40 | - |
- |
Vibrio splendidus 12B01 | A3UR41 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Vibrio splendidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | - |
Vibrio splendidus | unsaturated algino-oligosaccharides | - |
? | |
alginate | - |
Vibrio splendidus 12B01 | unsaturated algino-oligosaccharides | - |
? | |
additional information | isoforms AlyA and AlyB principally cleave the beta-1,4 bonds between beta-D-mannuronate and alpha-L-guluronate and subunits | Vibrio splendidus | ? | - |
? | |
additional information | isoforms AlyD and AlyE principally cleave the alpha-1,4 bonds involving alpha-L-guluronate subunits | Vibrio splendidus | ? | - |
? | |
additional information | isoforms AlyA and AlyB principally cleave the beta-1,4 bonds between beta-D-mannuronate and alpha-L-guluronate and subunits | Vibrio splendidus 12B01 | ? | - |
? | |
additional information | isoforms AlyD and AlyE principally cleave the alpha-1,4 bonds involving alpha-L-guluronate subunits | Vibrio splendidus 12B01 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 57500, calculated from sequence, 1 * 62000, SDS-PAGE | Vibrio splendidus |
monomer | 1 * 67400, calculated from sequence, 1 * 70000, SDS-PAGE | Vibrio splendidus |
Synonyms | Comment | Organism |
---|---|---|
AlyA | - |
Vibrio splendidus |
AlyB | - |
Vibrio splendidus |
V12B01_24254 | - |
Vibrio splendidus |
V12B01_24259 | - |
Vibrio splendidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 25 | - |
Vibrio splendidus |
25 | - |
- |
Vibrio splendidus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
alginate | pH 8.5, 25°C | Vibrio splendidus | |
3.7 | - |
alginate | pH 7.5, 25°C | Vibrio splendidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Vibrio splendidus |
8.5 | - |
- |
Vibrio splendidus |