Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Vibrio sp. W13 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, 80% of initial activity | Vibrio sp. W13 | |
Cu2+ | 1 mM, 81% of initial activity | Vibrio sp. W13 | |
Mn2+ | 1 mM, 75% of initial activity | Vibrio sp. W13 | |
Zn2+ | 1 mM, 66% of initial activity | Vibrio sp. W13 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate) | Km value 0.50 mg/ml, pH 8.0, 30°C | Vibrio sp. W13 | |
additional information | - |
alginate | Km value 0.67 mg/ml, pH 8.0, 30°C | Vibrio sp. W13 | |
additional information | - |
poly(alpha-(1,4)-L-guluronate) | Km value 1.04 mg/ml, pH 8.0, 30°C | Vibrio sp. W13 | |
additional information | - |
poly(beta-(1,4)-D-mannuronate) | Km value 6.9 mg/ml, pH 8.0, 30°C | Vibrio sp. W13 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 135% of initial activity | Vibrio sp. W13 | |
Co2+ | 1 mM, 156% of initial activity | Vibrio sp. W13 | |
Fe2+ | 1 mM, 148% of initial activity | Vibrio sp. W13 | |
Na+ | optimum concentration 0.3 M, about 2fold increase in activity compared to absence of NaCl | Vibrio sp. W13 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio sp. W13 | A0A0A1DYR7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein, purified by Ni-NTA Sepharose affinity chromatography | Vibrio sp. W13 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
457 | - |
pH 8.0, 30°C | Vibrio sp. W13 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alginate | - |
Vibrio sp. W13 | unsaturated algino-oligosaccharides | - |
? | |
additional information | AlgB mainly releases oligosaccharides with a degree of polymersiation of 2-5 from the different kinds of substrates in an endolytic manner | Vibrio sp. W13 | ? | - |
? | |
poly(alpha-(1,4)-L-guluronate) | reaction of EC 4.2.2.11 | Vibrio sp. W13 | 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? | |
poly(beta-(1,4)-D-mannuronate) | - |
Vibrio sp. W13 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) | - |
? | |
poly(beta-(1,4)-D-mannuronate/alpha-(1,4)-L-guluronate) | - |
Vibrio sp. W13 | 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-(1,4)-beta-oligo(beta-(1,4)-D-mannuronate) + 4-O-(4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl)-(1,4)-alpha-oligo(alpha-(1,4)-L-guluronate) | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54120, calculated from sequence, x * 55050, SDS-PAGE, mature enzyme | Vibrio sp. W13 |
Synonyms | Comment | Organism |
---|---|---|
AlgB | - |
Vibrio sp. W13 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Vibrio sp. W13 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
30 min, about 60% residual activity | Vibrio sp. W13 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Vibrio sp. W13 |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Vibrio sp. W13 | - |
- |
4.9 |