4.2.1.3: aconitate hydratase
This is an abbreviated version!
For detailed information about aconitate hydratase, go to the full flat file.
Word Map on EC 4.2.1.3
-
4.2.1.3
-
iron-sulfur
-
transferrin
-
tricarboxylic
-
dismutase
-
fe-s
-
succinate
-
tca
-
malate
-
citric
-
cardiac
-
rna-binding
-
neurodegenerative
-
frataxin
-
krebs
-
fumarase
-
friedreich
-
heme
-
ataxia
-
parkinson
-
overload
-
iron-dependent
-
alpha-ketoglutarate
-
stem-loops
-
fluorocitrate
-
bioenergetics
-
ferroportin
-
county
-
hepcidin
-
peroxynitrite
-
mnsod
-
fluoroacetate
-
georgia
-
cluster-containing
-
iron-deficient
-
iscu
-
iron-replete
-
iron-induced
-
iron-mediated
-
alabama
-
kennedy
-
itaconic
-
ferrochelatase
-
cubane
-
desulfurase
-
nadp-isocitrate
-
rna-protein
-
iron-related
-
soxrs
-
l-ferritin
-
isopropylmalate
-
medicine
-
environmental protection
-
synthesis
-
biotechnology
- 4.2.1.3
-
iron-sulfur
- transferrin
-
tricarboxylic
- dismutase
- fe-s
- succinate
- tca
- malate
-
citric
- cardiac
-
rna-binding
- neurodegenerative
- frataxin
-
krebs
- fumarase
- friedreich
- heme
- ataxia
- parkinson
- overload
-
iron-dependent
- alpha-ketoglutarate
-
stem-loops
- fluorocitrate
-
bioenergetics
-
ferroportin
-
county
- hepcidin
- peroxynitrite
- mnsod
- fluoroacetate
-
georgia
-
cluster-containing
-
iron-deficient
- iscu
-
iron-replete
-
iron-induced
-
iron-mediated
- alabama
-
kennedy
-
itaconic
-
ferrochelatase
- cubane
-
desulfurase
-
nadp-isocitrate
-
rna-protein
-
iron-related
-
soxrs
- l-ferritin
- isopropylmalate
- medicine
- environmental protection
- synthesis
- biotechnology
Reaction
Synonyms
Acn, AcnA, AcnA3, AcnB, ACO, Aco1, Aco2, Aco3, ACO4, acon, aconitase, aconitase 2, aconitase A, aconitase B, aconitase/2-methylaconitate hydratase, Aconitate hydratase, AH, c-acon, c-aconitase, CAA, cis-aconitase, citB, citrate hydro-lyase, cytoplasmic aconitase, cytoplasmic aconitase/iron regulatory protein 1 homolog, EC 4.2.1.4, Ferritin repressor protein, hydratase, aconitate, IP210, IRE-BP, Iron regulatory protein, iron regulatory protein 1, iron regulatory-like protein, iron-regulatory protein 1, iron-responsive element binding protein, IRP, IRP-1, IRP1, mACON, Major iron-containing protein, MICP, More, PfIRPa, SPBP4H10.15
ECTree
4.2.1.3 aconitate hydrataseAdvanced search results
results (
results (Engineering
Engineering on EC 4.2.1.3 - aconitate hydratase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
C450S
-
mutant is enzymatically inactive, glutamate auxotroph and accumulates citrate. Mutant strain exhibits overexpression of the citB promoter and accumulates high levels of aconitase protein. Mutant strain exhibits increased levels of citrate synthase protein. Mutant enzyme does not bind to the citrate synthase 5' leader RNA in vitro
C517A
-
enzymatically inactive mutant enzyme that still binds iron responsive elements
R740E/Q744E/F661L/I809T/V852A
-
sixfold increase in specific activity compared to wild-type. Mutant strain is defective in sporulation, affecting the expression of deltaK-dependent genes. Accumulation of transcriptional activator GerE mRNa and protein is delayed in the mutant
R741E
-
mutant is designed to be defective in RNA binding. Mutant strain is glutamate prototroph and accumulates citrate. Mutant strain exhibits overexpression of the citB promoter and accumulates high levels of aconitase protein. Mutant strain exhibits increased levels of citrate synthase protein. Mutant enzyme does not bind to the citrate synthase 5' leader RNA in vitro
R741E/Q745E
C459S
-
catalytically inactive mutant without its [4Fe-4S]-cluster. Mitochondrial morphological defects as a consequence of acon inactivation depend on its [4Fe-4S] cluster
S711A
-
citrate-to-isocitrate aconitase activity is about 70% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 90% of wild-type activity
S711D
-
no citrate-to-isocitrate aconitase activity, isocitrate-to-cis-aconitate activity is identical to wild-type activity
S711E
S711T
-
citrate-to-isocitrate aconitase activity is about 60% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 60% of wild-type activity
C538A
-
mutation of cysteine residue involved in the coordination of the [4Fe-4S] cluster, mutant shows no catalytic activity. Mutant displays a lower affinity for the IRE sequence than the wild-type aconitase as shown in gel shift assays
DELTA125-129
-
mutant shows lower activity compared to wild-type
R763E/Q767E
-
mutant shows no enzymatic activity, mutant does not bind at all to IRE-like structure as shown in gel shift assays
additional information
R741E/Q745E
-
site-directed mutagenesis, the mutant strain exhibits an increased enzymatic activity of aconitase comparing to that of the wild-type strain, because the aconitase protein expression level is significantly increased in the mutant strain
R741E/Q745E
-
site-directed mutagenesis, the mutant strain exhibits an increased enzymatic activity of aconitase comparing to that of the wild-type strain, because the aconitase protein expression level is significantly increased in the mutant strain
-
S711E
-
45% of the capacity to catalyze conversion of cis-aconitase into isocitrate, completely fails to bind RNA and to generate isocitrate from citrate
S711E
-
citrate-to-isocitrate aconitase activity is about 10% of the wild-type activity, isocitrate-to-cis-aconitate activity is about 20% of wild-type activity
additional information
transposon mutagenesis. In one disulfide 3,3-dithiodipropionic acid (DTDP)-negative (Jhw13b) and one DTDP-leaky (JhwAA14) mutant, Tn5::mob is mapped in a gene encoding a putative bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase (AcnB, EC 4.2.1.3). AcnB dehydrates 2-methylcitric acid to 2-methyl-cis-aconitic acid and subsequently hydrates it to 2-methylisocitric acid
additional information
-
transposon mutagenesis. In one disulfide 3,3-dithiodipropionic acid (DTDP)-negative (Jhw13b) and one DTDP-leaky (JhwAA14) mutant, Tn5::mob is mapped in a gene encoding a putative bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase (AcnB, EC 4.2.1.3). AcnB dehydrates 2-methylcitric acid to 2-methyl-cis-aconitic acid and subsequently hydrates it to 2-methylisocitric acid
additional information
-
transposon mutagenesis. In one disulfide 3,3-dithiodipropionic acid (DTDP)-negative (Jhw13b) and one DTDP-leaky (JhwAA14) mutant, Tn5::mob is mapped in a gene encoding a putative bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase (AcnB, EC 4.2.1.3). AcnB dehydrates 2-methylcitric acid to 2-methyl-cis-aconitic acid and subsequently hydrates it to 2-methylisocitric acid
-
additional information
-
enzyme knock-out plants have significantly less chlorosis after treatment with the superoxide-generating compound paraquat, show delayed induction of the antioxidant gene GST1 and inceased levels of chloroplastic CuZn superoxide dismutase 2 mRNA
additional information
-
loss-of-fucntion mutants of isoforms Aco1, Aco2, Aco3. Plants with mutations aco1 or aco3 show a clear decrease in cytosolic aconitase activity. None of the mutants is affected in respect of the accumulation of the ferritin transcript or protein response to iron excess
additional information
isoform IRP-1A specific overexpression in muscle results in pre-adult lethality
additional information
isoform IRP-1A specific overexpression in muscle results in pre-adult lethality
additional information
-
isoform IRP-1A specific overexpression in muscle results in pre-adult lethality
additional information
construction of a fusion protein of aconitase B and isocitrate dehydrogenase, ICDH and AcnB, i.e. ICDH-AcnB, structure determination of ICDH-AcnB, overview
additional information
-
naturally occuring IRP1 has no [Fe-S] cluster and is devoid of aconitase activity due to the absence of cysteine residues binding the [Fe-S] cluster in the active center
additional information
naturally occuring IRP1 has no [Fe-S] cluster and is devoid of aconitase activity due to the absence of cysteine residues binding the [Fe-S] cluster in the active center
additional information
-
naturally occuring IRP1 has no [Fe-S] cluster and is devoid of aconitase activity due to the absence of cysteine residues binding the [Fe-S] cluster in the active center
additional information
naturally occuring IRP1 has no [Fe-S] cluster and is devoid of aconitase activity due to the absence of cysteine residues binding the [Fe-S] cluster in the active center
additional information
-
virus-induced gene silencing of enzyme causes a 90% reduction in its activity, leading to stunting, spontaneous necrotic lesions, and increased resistance to paraquat. Silenced plants show less cell death after transient co-expression of the AvrPto and Pto proteins or the pro-apoptotoc protein Bax. Silenced plants expressing the Pto transgene display a delayed hypersensitive response and support higher levels of bacterial growth
additional information
-
naturally occuring IRP1 has no [Fe-S] cluster and is devoid of aconitase activity due to the absence of cysteine residues binding the [Fe-S] cluster in the active center
additional information
naturally occuring IRP1 has no [Fe-S] cluster and is devoid of aconitase activity due to the absence of cysteine residues binding the [Fe-S] cluster in the active center
additional information
-
overexpression of enzyme in Escherichia coli. Presence of co-expressed GroEL reduces the aconitase over-expression drastically, however, exogenous GroEL and GroES together compensate this reduction. For over-expressing cells, growth-rate decreases by 30% at 25°C, however, in presence of co-expressed GroEL and GroES the growth rate of aconitase producing cells is enhanced by 30% at 37°C
additional information
-
transgenic expression of yeast enzyme in Bacillus subtilis aconitase null mutant restores aconitase activity and glutamate prototrophy, but only partially restores sporulation. Late sporulation gene expression in the transgenic strain is delayed
additional information
-
the gene aco1 deficient mutant aco1DELTA shares several growth phenotypes as well as patterns of specific protein expression with a Saccharomyces cerevisiae mutants lacking mitochondrial NAD+-specific isocitrate dehydrogenase idhDELTA, these phenotypes are eliminated by co-disruption of the CIT1 gene encoding mitochondrial citrate synthase, effects of citrate, iron, and pH, overview
additional information
-
construction of diverse deletion mutants of aconitase, overview. Deletion of six C-terminal amino acids does not eliminate enzymatic activity but abolishes dual targeting
additional information
-
construction of diverse deletion mutants of aconitase, overview. Deletion of six C-terminal amino acids does not eliminate enzymatic activity but abolishes dual targeting
-
additional information
-
the gene aco1 deficient mutant aco1DELTA shares several growth phenotypes as well as patterns of specific protein expression with a Saccharomyces cerevisiae mutants lacking mitochondrial NAD+-specific isocitrate dehydrogenase idhDELTA, these phenotypes are eliminated by co-disruption of the CIT1 gene encoding mitochondrial citrate synthase, effects of citrate, iron, and pH, overview
-
