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4.2.1.3: aconitate hydratase

This is an abbreviated version!
For detailed information about aconitate hydratase, go to the full flat file.

Word Map on EC 4.2.1.3

Reaction

citrate
=
cis-aconitate
+
H2O

Synonyms

Acn, AcnA, AcnA3, AcnB, ACO, Aco1, Aco2, Aco3, ACO4, acon, aconitase, aconitase 2, aconitase A, aconitase B, aconitase/2-methylaconitate hydratase, Aconitate hydratase, AH, c-acon, c-aconitase, CAA, cis-aconitase, citB, citrate hydro-lyase, cytoplasmic aconitase, cytoplasmic aconitase/iron regulatory protein 1 homolog, EC 4.2.1.4, Ferritin repressor protein, hydratase, aconitate, IP210, IRE-BP, Iron regulatory protein, iron regulatory protein 1, iron regulatory-like protein, iron-regulatory protein 1, iron-responsive element binding protein, IRP, IRP-1, IRP1, mACON, Major iron-containing protein, MICP, More, PfIRPa, SPBP4H10.15

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.3 aconitate hydratase

Storage Stability

Storage Stability on EC 4.2.1.3 - aconitate hydratase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 25% w/v glycerol, stable for several weeks
Cucurbita sp.
-
-20°C, enzyme frozen after dialysis against 0.05 M Tris-HCl, pH 7.5, complete inactivation after 10 days
-
4°C, 24 h, pH 5.5-9.0, stable
-
4°C, activity of the highly purified cytosolic enzyme decreases by 15-20% during the first 24 h of storage and then by 5% every 24 h, the mitochondrial enzyme decreases 35-40% during the first 24 h and then by 15% every 24 h
-