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Literature summary for 4.2.1.3 extracted from

  • Gao, W.; Dai, S.; Liu, Q.; Xu, H.; Bai, Y.; Qiao, M.
    Effect of site-directed mutagenesis of citB on the expression and activity of Bacillus subtilis aconitase (2011), Mikrobiologiia, 79, 774-778.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha, and in Bacillus subtilis strains GWH1025 and GWH1026, respectively Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
R741E/Q745E site-directed mutagenesis, the mutant strain exhibits an increased enzymatic activity of aconitase comparing to that of the wild-type strain, because the aconitase protein expression level is significantly increased in the mutant strain Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
gene citB
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Bacillus subtilis NK1010
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gene citB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information amino acid residues Arg741 and Gln745 play great role in the aconitase function Bacillus subtilis ?
-
?
additional information amino acid residues Arg741 and Gln745 play great role in the aconitase function Bacillus subtilis NK1010 ?
-
?

Synonyms

Synonyms Comment Organism
aconitase
-
Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Bacillus subtilis

General Information

General Information Comment Organism
physiological function aconitase is a bifunctional enzyme, which can not only interconvert citrate and isocitrate, but also has the RNA binding function similar to the eukaryotic protein IRP-1, iron regulatory protein 1 Bacillus subtilis