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4.2.1.3: aconitate hydratase

This is an abbreviated version!
For detailed information about aconitate hydratase, go to the full flat file.

Word Map on EC 4.2.1.3

Reaction

citrate
=
cis-aconitate
 +
H2O

Synonyms

Acn, AcnA, AcnA3, AcnB, ACO, Aco1, Aco2, Aco3, ACO4, acon, aconitase, aconitase 2, aconitase A, aconitase B, aconitase/2-methylaconitate hydratase, Aconitate hydratase, AH, c-acon, c-aconitase, CAA, cis-aconitase, citB, citrate hydro-lyase, cytoplasmic aconitase, cytoplasmic aconitase/iron regulatory protein 1 homolog, EC 4.2.1.4, Ferritin repressor protein, hydratase, aconitate, IP210, IRE-BP, Iron regulatory protein, iron regulatory protein 1, iron regulatory-like protein, iron-regulatory protein 1, iron-responsive element binding protein, IRP, IRP-1, IRP1, mACON, Major iron-containing protein, MICP, More, PfIRPa, SPBP4H10.15

ECTree

     4 Lyases
         4.2 Carbon-oxygen lyases
             4.2.1 Hydro-lyases
                4.2.1.3 aconitate hydratase

Cloned

Cloned on EC 4.2.1.3 - aconitate hydratase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AcnB domain 5-4 polypeptide is expressed in Escherichia coli as a GST-AcnB5-4 fusion protein
-
cytoplasmic aconitase/iron regulatory protein 1 expression analysis, overview
-
expressed in Escherichia coli
expressed in Escherichia coli as a His-tagged fusion protein
-
expressed in Escherichia coli as functional enzyme
-
expression in Bacillus subtilis
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)
expression in HEK 293 cells
expression of N-terminally His-tagged aconitase in Escherichia coli strain BL21(DE3), alternative expression method uses overexpression of aconitase from plasmid pTYB2-acn, resulting in an aconitase containing only an additional glycine residue at the C-terminus
-
expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha, and in Bacillus subtilis strains GWH1025 and GWH1026, respectively
-
expression of wild-type enzyme and diverse pount and deletion mutants
-
gene AcnA, overexpression of the His-tagged protein in an ASKA library, i.e. the 'a complete set of Escherichia coli K-12 ORF archive library
gene acnB, expression of wild-type enzyme and the fusion protein ICDH-AcnB in Escherichia coli strain BL21 (DE3)
gene ACnB, overexpression of the His-tagged protein in an ASKA library
gene ACO1, overexpression in strain H222, high-level expression of ACO in the ACO1 multicopy integrative transformant results in a shift of the citric acid/isocitric acid product pattern into the direction of isocitric acid
-
gene Ccaco1, DNA and amino acid sequence determination, sequence comparisons and phylogenetic analysis
gene Ccaco2, DNA and amino acid sequence determination, sequence comparisons and phylogenetic analysis
gene Pbaco, quantitative real-time RT-PCR expression analysis, recombinant expression in Saccharomyces cerevisiae, ACO is present in the extracellular fluid, cell wall enriched fraction, mitochondria, cytosol and peroxisomes of yeast cells
-
overexpression in Escherichia coli, His-tag
the recombinant yeast mitochondrial aconitase is expressed in Escherichia coli as soluble, biologically active enzyme
-
yeast mitochondrial aconitase is expressed in Escherichia coli by cultivation in a bioreactor at different temperatures. Chaperones GroEL and GroES are co-expressed
-