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Literature summary for 4.2.1.3 extracted from
- First biochemical characterization of a methylcitric acid cycle from Bacillus subtilis strain 168 (2017), Biochemistry, 56, 5698-5711 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (Z)-2-methylaconitate | Bacillus subtilis | the enzyme is involved in the methylcitric acid cycle | 2-methylisocitrate | - |
? |  |
| (Z)-2-methylaconitate | Bacillus subtilis 168 | the enzyme is involved in the methylcitric acid cycle | 2-methylisocitrate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | P09339 | - |
- |
| Bacillus subtilis 168 | P09339 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (Z)-2-methylaconitate | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis | 2-methylisocitrate | - |
? | |
| (Z)-2-methylaconitate | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis 168 | 2-methylisocitrate | - |
? | |
| (Z)-2-methylaconitate + H2O | - |
Bacillus subtilis | 2-methylisocitrate | - |
? | |
| (Z)-2-methylaconitate + H2O | - |
Bacillus subtilis 168 | 2-methylisocitrate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| aconitase/2-methylaconitate hydratase | - |
Bacillus subtilis |
| citB | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bacillus subtilis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| iron-sulfur centre | - |
Bacillus subtilis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme is involved in the methylcitric acid cycle | Bacillus subtilis |
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Release 2023.1 (January 2023)
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