Cloned (Comment) | Organism |
---|---|
gene acnB, expression of wild-type enzyme and the fusion protein ICDH-AcnB in Escherichia coli strain BL21 (DE3) | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a fusion protein of aconitase B and isocitrate dehydrogenase, ICDH and AcnB, i.e. ICDH-AcnB, structure determination of ICDH-AcnB, overview | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-aconitate + H2O | Escherichia coli | - |
isocitrate | - |
? | |
citrate | Escherichia coli | - |
cis-aconitate + H2O | - |
? | |
additional information | Escherichia coli | weak interactions, which affects structure and function of the proteins, of aconitase B and isocitrate dehydrogenase, overview. Two monomeric AcnB regions associate with the homodimeric ICDH region. The versatile architecture of AcnB may alter the metabolic process involving the Krebs cycle | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P36683 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzymes from Escherichia coli strain BL21(DE3) by gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-aconitate + H2O | - |
Escherichia coli | isocitrate | - |
? | |
citrate | - |
Escherichia coli | cis-aconitate + H2O | - |
? | |
additional information | weak interactions, which affects structure and function of the proteins, of aconitase B and isocitrate dehydrogenase, overview. Two monomeric AcnB regions associate with the homodimeric ICDH region. The versatile architecture of AcnB may alter the metabolic process involving the Krebs cycle | Escherichia coli | ? | - |
? | |
additional information | the active sites within ICDH-AcnB catalyze the three consecutive reactions, in which citrate is converted to 2-oxoglutarate, via cisaconitate and isocitrate | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | structural analysis by X-ray scattering and modelling of wild-type and mutant enzymes, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
AcnB | - |
Escherichia coli |
aconitase B | - |
Escherichia coli |
More | AcnB is a member of the aconitase family | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |